| 質粒名稱: | pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 101203 |
| 存儲實驗室: | Jennifer Doudna, Keith Joung |
|
載體骨架基本信息 |
|
| 載體名稱: | pCT10 |
| 載體出品公司: | Doudna lab |
| 空載體大小: | -- |
| 完整質粒大小: | 9437 bp |
| 載體修飾: | -- |
| 載體類型: | 細菌表達 |
| 篩選標記: | -- |
| 載體抗性: | 氨芐青霉素，100μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368 |
| 物種來源: | Streptococcus pyogenes |
| 插入大小: | 951 bp |
| 基因突變: | C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D1368 |
| GenBank編號 | -- |
| 啟動子: | T7 |
| 標簽/融合蛋白: | 10xHis（主干上的N端子） MBP（主干上的N終端）TEV站點（主干上的N終端） |
| 克隆方法: | Gibson Cloning |
| 5’克隆位點: | -- |
| 3’克隆位點: | -- |
| 5’測序引物 | T7 |
| 3’測序引物 | T7-term |
|
文獻引用方法 |
|
| 材料和方法部分: | pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant was a gift from Jennifer Doudna & Keith Joung (Addgene plasmid # 101203 ; //n2t.net/addgene:101203 ; RRID:Addgene_101203) |
| 參考文獻部分: | Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. 10.1038/nature24268 PubMed 28931002 |
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3
買家導航
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3載體質粒基本信息
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3質粒圖譜載體圖譜和pJSC038 - Bacterial expression plasmid for SpCas9∆REC3載體序列質粒序列多克隆位點信息
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3質粒載體簡介
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3質粒序列載體序列
LOCUS pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant 9437 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant.
ACCESSION .
VERSION .
KEYWORDS pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FR...
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9437)
AUTHORS Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington
LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
TITLE Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
JOURNAL Nature. 2017 Sep 20. doi: 10.1038/nature24268.
PUBMED 28931002
REFERENCE 2 (bases 1 to 9437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
//gt12365.cn
FEATURES Location/Qualifiers
source 1..9437
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 462..481
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 565..584
/label=T7
/note="T7 promoter, forward primer"
promoter 565..583
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 672..694
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 714..743
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
CDS 753..1853
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 1827..1850
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 1908..1928
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
primer_bind complement(5531..5549)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 5545..5592
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(5958..5986)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(6013..6031)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6099..6203
/gene="bla"
/label=AmpR promoter
CDS 6204..7064
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6422..6441)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7235..7823
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7724..7743
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7977..7994
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8009..8148
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8074..8093)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8234..8256
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(8250..8441)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
61 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
121 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
181 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
241 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag
301 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
361 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
421 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
481 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
541 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
601 cctctagtgc cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt
661 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc
721 accatcacca tcaccatcac catggttctt ctatgaaaat cgaagaaggt aaactggtaa
781 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga
841 aagataccgg aattaaagtc accgttgagc atccggataa actggaagag aaattcccac
901 aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg
961 gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc
1021 tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg
1081 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct
1141 gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt
1201 tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt
1261 tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcaa
1321 aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg
1381 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc
1441 cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga
1501 ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg
1561 ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag
1621 gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg
1681 aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag aaaggtgaaa
1741 tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca
1801 acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga
1861 gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggaa aacctgtact
1921 tccaatccaa tgcaatggat aagaaatact caataggctt agatatcggc acaaatagcg
1981 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg
2041 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg
2101 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga
2161 agaatcgtat ttcttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata
2221 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc
2281 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct
2341 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt
2401 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc
2461 ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat
2521 ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat
2581 tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg
2641 gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt
2701 ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag
2761 ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt
2821 tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc
2881 ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa
2941 aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatgta
3001 aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta
3061 tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg
3121 aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact
3181 tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca
3241 atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg
3301 cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat
3361 ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga
3421 caaacggtgg aagtgtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag
3481 ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg
3541 tcaaagtaat ggggcggcat aagccagaaa atatagttat tgaaatggca cgtgaaaatc
3601 agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta
3661 tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa
3721 atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat
3781 tagatattaa tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta
3841 aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatgcg
3901 ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa
3961 acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt
4021 tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca
4081 ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata
4141 aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa
4201 aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt
4261 atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt
4321 ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag
4381 aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa
4441 cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg
4501 aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt
4561 ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg
4621 agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc
4681 caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta
4741 aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa
4801 ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata
4861 aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa
4921 acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc
4981 tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta
5041 gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg
5101 agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag
5161 ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa
5221 atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg
5281 atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta
5341 tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg
5401 actaataaca ttggaagtgg ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg
5461 ccggtaccac gcgtgcgctc tgatccggct gctaacaaag cccgaaagga agctgagttg
5521 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
5581 aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc
5641 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
5701 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
5761 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
5821 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
5881 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
5941 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt
6001 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
6061 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
6121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
6181 aataacattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
6241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
6301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
6361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
6421 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
6481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
6541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
6601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
6661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
6721 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
6781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
6841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
6901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
6961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
7021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
7081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
7141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
7201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
7261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
7321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
7381 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
7441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
7501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
7561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
7621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
7681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
7741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
7801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
7861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
7921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
7981 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
8041 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
8101 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
8161 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
8221 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
8281 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
8341 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
8401 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
8461 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
8521 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
8581 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
8641 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
8701 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
8761 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
8821 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
8881 gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc
8941 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
9001 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
9061 aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg
9121 cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc
9181 tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg
9241 taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
9301 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
9361 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca
9421 tgccggcgat aatggcc
//