| 質粒名稱: | AAV-shRNA_Tet3 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 85740 |
| 存儲實驗室: | Hongjun Song |
|
載體骨架基本信息 |
|
| 載體名稱: | AAV |
| 載體出品公司: | Clontech |
| 完整質粒大小: | 5739bp |
| 載體類型: | 哺乳細胞表達、AAV |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | Tet3 |
| 物種來源: | M. musculus (mouse) |
| 標簽/融合蛋白: |
Tag / Fusion Protein
EYFP (C terminal on insert)
|
|
文獻引用方法 |
|
| 材料和方法部分: | AAV-shRNA_Tet3 was a gift from Hongjun Song (Addgene plasmid # 85740 ; //n2t.net/addgene:85740 ; RRID:Addgene_85740) |
| 參考文獻部分: |
Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair. Yu H, Su Y, Shin J, Zhong C, Guo JU, Weng YL, Gao F, Geschwind DH, Coppola G, Ming GL, Song H. Nat Neurosci. 2015 Jun;18(6):836-43. doi: 10.1038/nn.4008. Epub 2015 Apr 27. 10.1038/nn.4008 PubMed 25915473
|
買家導航
AAV-shRNA_Tet3載體質粒基本信息
AAV-shRNA_Tet3質粒圖譜載體圖譜和AAV-shRNA_Tet3載體序列質粒序列多克隆位點信息
AAV-shRNA_Tet3質粒載體簡介
AAV-shRNA_Tet3質粒序列載體序列
LOCUS AAV-shRNA_Tet3 5736 bp DNA circular SYN 25-AUG-2024
DEFINITION shRNA against mouse Tet3.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5736)
AUTHORS Yu H, Su Y, Shin J, Zhong C, Guo JU, Weng YL, Gao F, Geschwind DH,
Coppola G, Ming GL, Song H
TITLE Tet3 regulates synaptic transmission and homeostatic plasticity via
DNA oxidation and repair.
JOURNAL Nat Neurosci. 2015 Jun;18(6):836-43. doi: 10.1038/nn.4008. Epub 2015
Apr 27.
PUBMED 25915473
REFERENCE 2 (bases 1 to 5736)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4008";
journalName: "Nat Neurosci"; date: "2015-06"; volume: "18"; issue:
"6"; pages: "836-43"
FEATURES Location/Qualifiers
source 1..5736
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..249
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 793..1512
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(838..859)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1099..1118)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1446..1467
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1534..2122
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1587..1607)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2005..2016)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2154..2630
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(2353..2372)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 2670..2810
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 2670..2799
/label=AAV2 ITR
rep_origin 2885..3340
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2972..2991)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3182..3203
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3357..3376)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3476..3498
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3536..3554)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3622..3726
/gene="bla"
/label=AmpR promoter
CDS 3727..4587
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3945..3964)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4758..5346
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5247..5266
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
repeat_region 5408..5537
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaggatccg gctccaacga gaagctattt ggaagcttgc aaatagcttc tcgttggagc
301 ctttttttct agagggatca tctaatcaag cacaaataag gggcgtgtaa cacaaaagcc
361 agcgaccctt tccaatgcaa atcaaacttg caattctttg ccgtttttat catttaagtg
421 tcggcttaag gtccactatc agatgtaaac agccttatct aacaaaggta tcattacatt
481 ctgaaattct caggcatgca agctagctta tgacgcacta gggagtgcca cccttccttt
541 cgccctaact tcgtgataac tcgcgcgttt cactcaacag ctgcatccgc cctagtgcta
601 ctgggagttg tagtatacaa gacgcttaca ggctgaatgt tctgtcaaga ccccgcctct
661 agcactttgg gaattctgga cttgatgatg tcatggttaa tccccgccca gtagaggcgg
721 ctatataaag ggtggttaag gcccggttcg ctctcttcct caccgggtct gcggcgagtt
781 ctagctgtcg acatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
841 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
901 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
961 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
1021 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
1081 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
1141 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1201 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1261 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1321 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1381 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
1441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1501 ctgtacaagt aagatatcat caagcttatc gataatcaac ctctggatta caaaatttgt
1561 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
1621 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
1681 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
1741 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
1801 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
1861 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
1921 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
1981 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
2041 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
2101 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
2161 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
2221 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
2281 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
2341 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
2401 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
2461 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
2521 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
2581 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
2641 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
2701 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
2761 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
2821 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
2881 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2941 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
3001 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
3061 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
3121 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
3181 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
3241 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
3301 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
3361 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
3421 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
3481 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
3541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
3601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
3661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
3721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
3781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
3841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
3901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
3961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
4021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
4081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
4141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
4201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
4261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
4321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
4381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
4441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
4501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
4561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
4621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
4681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
4741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
4921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
5041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
5101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
5161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
5221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
5281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
5401 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
5461 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
5521 catcactagg ggttcctgcg gccgcacgcg taagctttgc aaagatggat aaagttttaa
5581 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
5641 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
5701 ggggtcggca attgaaccgg tagatctggg caggaa
//
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