| 質粒名稱: | BB3nK_ext_AD |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 108687 |
| 存儲實驗室: | Brigitte Gasser |
|
載體骨架基本信息 |
|
| 載體名稱: | BB3nK_ext_AD |
| 載體出品公司: | -- |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | 2642 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 細菌表達，酵母表達 |
| 篩選標記: | G418 |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | -- |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | BB3nK_ext_AD was a gift from Brigitte Gasser (Addgene plasmid # 108687 ; //n2t.net/addgene:108687 ; RRID:Addgene_108687) |
買家導航
BB3nK_ext_AD載體質粒基本信息
BB3nK_ext_AD質粒圖譜載體圖譜和BB3nK_ext_AD載體序列質粒序列多克隆位點信息
BB3nK_ext_AD質粒載體簡介
BB3nK_ext_AD質粒序列載體序列
LOCUS BB3nK_ext_AD 2642 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid for assembly of donor DNA templates consisting of three
expression units.
ACCESSION .
VERSION .
KEYWORDS BB3nK_ext_AD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2642)
AUTHORS Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R.
TITLE CRISPR/Cas9-mediated homology directed genome editing in Pichia
pastoris
JOURNAL (2018) In Press
REFERENCE 2 (bases 1 to 2642)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..2642
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(62..650)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(142..161)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
gene 785..2141
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 785..1128
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 1129..1938
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1196..1215)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 1944..2141
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
ORIGIN
1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
721 gcgatcgaag acccagctgg agaccctcga gggatccggt ctccgatcgg gtcttcagct
781 catggacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
841 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
901 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacgtg
961 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
1021 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
1081 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa
1141 aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
1201 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
1261 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
1321 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
1381 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca
1441 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
1501 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
1561 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
1621 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
1681 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
1741 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
1801 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
1861 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
1921 ctcgatgagt ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt
1981 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt
2041 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa
2101 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact ggcttgtaag caatctggac
2161 aattatgtaa gcgggttacg taaacagtta tgtaagcaga aaaatttcaa acgacaaaac
2221 ttggggtcta cagacacagt agccagaaga ttgcactacc attcgactcc tcatgaccca
2281 ctctttcgat ccatgtagtt aggttaccgt ttttcctaat atttaaggat gttgaaaatt
2341 cattttcatt tttttcgttt ttaagatttt ctcacaactc ttccaaagat tactagttga
2401 cttttcaaat atttagggta tttttctcac tttttcctag caaactccaa ttggtgggtt
2461 cagtgcaatg gagtatcacc ttgcaaccac aacgtaatag ctaacttgtg gccaccatgt
2521 ctggttgtag agataattgg attctaatgt ggatcacatg actactcacg tgtcaaaaac
2581 ccaacctgac ttggcccagc ttagcaagaa tatttcgaat ccactcttgt ggcctagtgg
2641 ac
//
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