LOCUS       EGFP-Rab7A Sequencing Result	6780 bp 	DNA	SYN
DEFINITION  EGFP-Rab7A Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="EGFP-Rab7A Sequencing Result"
                     /mol_type="other DNA"
     promoter        143..172
                     /label="lac_promoter"
     misc_feature    186..208
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /label="M13_reverse_primer"
     misc_feature    complement(281..300)
                     /label="EBV_rev_primer"
     CDS             514..1050
                     /label="ORF frame 1"
     CDS             complement(565..1359)
                     /label="ORF frame 1"
     gene            complement(568..1347)
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     promoter        complement(1474..1742)
                     /label="SV40_promoter"
     misc_feature    complement(1494..1513)
                     /label="SV40pro_F_primer"
     rep_origin      complement(1498..1575)
                     /label="SV40_origin"
     misc_feature    1545..1760
                     /label="SV40_enhancer"
     misc_feature    1740..1760
                     /label="pBABE_3_primer"
     rep_origin      complement(1874..2180)
                     /label="f1_origin"
     terminator      complement(2243..2470)
                     /label="bGH_PA_terminator"
     misc_feature    2456..2473
                     /label="BGH_rev_primer"
     promoter        2492..2509
                     /label="Sp6_promoter"
     misc_feature    2493..2510
                     /label="Sp6_primer"
     CDS             complement(2546..3949)
                     /label="ORF frame 3"
     CDS             complement(3159..4070)
                     /label="ORF frame 2"
     misc_feature    complement(3197..3223)
                     /label="HA"
     gene            complement(3233..3946)
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3275..3296)
                     /label="EGFP_C_primer"
     misc_feature    complement(3737..3766)
                     /label="Y66 (EGFP)"
     misc_feature    3883..3904
                     /label="EGFP_N_primer"
     promoter        complement(3963..3981)
                     /label="T7_promoter"
     misc_feature    complement(4055..4075)
                     /label="CMV_fwd_primer"
     misc_feature    complement(4241..4528)
                     /label="CAG_enhancer"
     promoter        4910..4938
                     /label="AmpR_promoter"
     gene            4980..5840
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             4980..5840
                     /label="ORF frame 3"
     rep_origin      5995..6614
                     /label="pBR322_origin"
ORIGIN
    1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC
   61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
  121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
  181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT
  241 AGCTAGAGGT CGACGGTATA CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC
  301 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT
  361 TGTAACCATT ATAAGCTGCA ATAAACAAGT TGGGGTGGGC GAAGAACTCC AGCATGAGAT
  421 CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT
  481 CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG
  541 TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT
  601 GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC
  661 GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GGTCCGCCAC
  721 ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG
  781 CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC GCGCCTTGAG
  841 CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC
  901 GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC
  961 GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA
 1021 TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA
 1081 TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC
 1141 CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCCTGC AGTTCATTCA GGGCACCGGA
 1201 CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC
 1261 ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC
 1321 GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT
 1381 CTCTTGATCA GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG
 1441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCAGAGG CGGCCTCGGC
 1501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG
 1561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG
 1621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA
 1681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
 1741 CCCTAACTGA CACACATTCC ACAGAATTAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA
 1801 TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG
 1861 ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC
 1921 AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC
 1981 TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC
 2041 CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA
 2101 GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC
 2161 ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTGGG GATACCCCCT AGAGCCCCAG
 2221 CTGGTTCTTT CCGCCTCAGA AGCCATAGAG CCCACCGCAT CCCCAGCATG CCTGCTATTG
 2281 TCTTCCCAAT CCTCCCCCTT GCTGTCCTGC CCCACCCCAC CCCCCAGAAT AGAATGACAC
 2341 CTACTCAGAC AATGCGATGC AATTTCCTCA TTTTATTAGG AAAGGACAGT GGGAGTGGCA
 2401 CCTTCCAGGG TCAAGGAAGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
 2461 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC
 2521 TCTAGATGCA TGCTCGAGCG GCCGCTTAGC AACTGCAGCT TTCTGCCGAG GCCTTGGCCC
 2581 GGTCATTCTT GTCCAGTTTG ATAGGTTCAG GAAATTCGTT GTACAGCTCC ACCTCCGTTT
 2641 CCTGCTTAAG TGCATTCCGT GCAATCGTCT GGAACGCCTG CTCCACGTTG ATGGCCTCCT
 2701 TGGCACTGGT CTCAAAGTAG GGAATGTTGT TTTTGCTGTA GCACCAGGCC TGTGCCCGCT
 2761 TTGTGGCCAC TTGTCTGTTT TCGAGGTCAA TCTTGTTTCC CAACACAACA AATGGGAAGT
 2821 TTTCAGGATC TCGGGGACTG GCCTGGATGA GAAACTCATC TCTCCAGCTA TCTAGGGTTT
 2881 TGAATGTGTT GGGGGCAGTC ACATCAAATA CCAGAACGCA GCAGTCTGCA CCTCTGTAGA
 2941 AGGCCACACC GAGAGACTGG AACCGTTCCT GTCCTGCTGT GTCCCATATC TGCATTGTGA
 3001 CTAGCCTGTC ATCCACCATC ACCTCCTTGG TCAGAAAGTC AGCTCCTATT GTGGCTTTGT
 3061 ACTGATTGCT GAATTTCTTA TTCACATACT GGTTCATGAG TGATGTCTTC CCGACTCCAG
 3121 AATCTCCCAG GATGATAACC TTCAGCAACA CTTTCTTCCT AGAGGTACCA GCGTAATCCG
 3181 GAACATCGTA TGGGTAAGCG TAATCCGGAA CATCGTATGG GTACATGGAT CCCTTGTACA
 3241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
 3301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG
 3361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
 3421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT
 3481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
 3541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
 3601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
 3661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
 3721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
 3781 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
 3841 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
 3901 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATA AGCTTGGGTC
 3961 TCCCTATAGT GAGTCGTATT AATTTCGATA AGCCAGTAAG CAGTGGGTTC TCTAGTTAGC
 4021 CAGAGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT
 4081 GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC
 4141 CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC
 4201 CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG
 4261 TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT
 4321 TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC
 4381 AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC
 4441 GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG
 4501 CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT
 4561 AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA ATCAATGTCA ACGCGTATAT
 4621 CTGGCCCGTA CATCGCGAAG CAGCGCAAAA CGCCTAACCC TAAGCAGATT CTTCATGCAA
 4681 TTGTCGGTCA AGCCTTGCCT TGTTGTAGCT TAAATTTTGC TCGCGCACTA CTCAGCGACC
 4741 TCCAACACAC AAGCAGGGAG CAGATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA
 4801 CTGAGAGTGC ACCATAGGGG ATCGGGAGAT CTCCCGATCC GTCGACGTCA GGTGGCACTT
 4861 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
 4921 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
 4981 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
 5041 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
 5101 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
 5161 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
 5221 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
 5281 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
 5341 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
 5401 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
 5461 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
 5521 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
 5581 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
 5641 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
 5701 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
 5761 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
 5821 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
 5881 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
 5941 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
 6001 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
 6061 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
 6121 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
 6181 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
 6241 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
 6301 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
 6361 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
 6421 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
 6481 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
 6541 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
 6601 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
 6661 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
 6721 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG

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