LOCUS       Flag-SENP1                6605 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Flag-SENP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Cheng J, Kang X, Zhang S, Yeh ET
  TITLE     SUMO-specific protease 1 is essential for stabilization of HIF1alpha
            during hypoxia.
  JOURNAL   Cell. 2007 Nov 2. 131(3):584-95.
  PUBMED    17981124
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     235..254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        235..253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(292..621)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      306..441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(360..379)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     606..626
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    650..1272
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     1054..1073
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(3260..3283)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3317..3520)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3346..3366)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3521..3900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(4061..4078)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4061..4077)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4070..4092)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4218..4673)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4355..4376)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4567..4586
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4700..4804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4805..5665
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5023..5042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5836..6424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6325..6344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6578..6595
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttaatac
      241 gactcactat agggaattaa ctttctggtt tttcagttcc tcgaggagct ttttgcaaaa
      301 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      361 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      421 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      481 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      541 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      601 tccacaccct aactgacaca cattccacag ccaagctggc cgcgtaccca attcaacagg
      661 catctactga gtggacccaa cgcatgagag gacagtgcca agcaagcaac tcaaatgtcc
      721 caccggttgg gcatggccag gtagcctatg ctgtgtctgg acgtcctcct gctggtatag
      781 ttattttaaa atcagaagga cagggaaggg agcagtggtt cacgcctgta atcccagcaa
      841 tttgggaggc caaggtgggt agatcacctg agattaggag ttggagacca gcctggccaa
      901 tatggtgaaa ccccgtctct accaaaaaaa caaaaattag ctgagcctgg tcatgcatgc
      961 ctggaatccc aacaactcgg gaggctgagg caggagaatc gcttgaaccc aggaggcgga
     1021 gattgcagtg agccaagatt gtgccactgc actccagctt ggttcccaat agaccccgca
     1081 ggccctacag gttgtcttcc caacttgccc cttgctccat accacccccc tccaccccat
     1141 aatattatag aaggacacct agtcagacaa aatgatgcaa cttaatttta ttaggacaag
     1201 gctggtgggc actggagtgg caacttccag ggccaggaga ggcactgggg aggggtcaca
     1261 gggatgccac ccgggatcct ctagagtcga ctcatcacaa gagttttcgg tggaggatct
     1321 cccagaccat ccgcttccgg aagtatggca tgtgttgctg tgcgaagttg attggtctgt
     1381 ctttggtaat acagtcagca tatttgcagg caaacatccc acagtcactt ccattcatct
     1441 gctgaggaat ctcctggctt ttcttgctga aaagctgcca gccattggtg tcaaactctt
     1501 tccttttctt gtcaatgctt tcttgcttta ggtattgcaa gagtattctg caggcttcat
     1561 tgtttatccc acccatggag tcgtaatagg taatattctt ctttctaaag tccacaacag
     1621 ctagacacca gtgtactccc aggtgaatgg gcaccaaaag aatgtcaaca gaaaatacat
     1681 ctactttctt tgtccaacgt ttcactgcct gataaccagc cgtttttaat ttagtgaaga
     1741 aaaaggtatt aaatgcatgc acacttggca agcccttctc tttacttcgc tccatcagca
     1801 tattcatgta gaaattgatg atctcatcat tgagccaatt cagatggttt agagtttgaa
     1861 tatctttgcg tgtaatggtc aggcgaaatg cttcactgag aacttcatcc tgattcccat
     1921 tacgaaatac attctttatt tctttctcca tttcctctgt aatttcagga aattcatctt
     1981 cactatcagt taatttatga cctttttttt gtgtttcttg gacaacagta acaggaatct
     2041 ccttttcaag aggtacacga agatgtagtt ctactgaatc atgtactgaa tgttcccgct
     2101 cctgcaatct ctggttttga agctgtaagg ccaatgcctt ctgttcttca atctggcgca
     2161 atctttctcg tgctcgagaa tcataaacac tagttaattc tttgatccac agctctgcct
     2221 ggaagaaagt agaactggga gttggagtct gggaatcttt cactttcagt aaaatcacag
     2281 agtctgatcc ttcagattgt gtatttgaag ctgctaagtt atctggctga tgtggaacag
     2341 agtggtgatg atggggatga tgaagagtac cagaatcttt ggatccaaat gcaacatctg
     2401 gggtataaga agatagggaa tatacactgt gggacagctg ttcctggtta gttaaaatgc
     2461 tggcagatcc agatgatgaa gtatcagagc caatgatctg agatgcacaa gagcttccat
     2521 ttttaaacag tgagtctttc aaagtatttt tactggaact aagacatcga gacaggtgta
     2581 aaggaaaatg tgtggtgggt ttggctatag caaactgttt ccctgtgacc atctgtagca
     2641 gctgtctgta aaactctctt tcttcttctt gaactgtctc ttctgctgta ctaacatgtc
     2701 gcctctgagt tttcttgggg ctcaaaagac ttcgacgaca tgaaccactc caagatggac
     2761 ttggaacagg tttaatagga aaagattttt catatgcaga tacatggcag tgatggtttg
     2821 actttcccgc aaaactgttt gataatccac ttgaggtctt tcgggtttcg aggtaaagac
     2881 ttcggctgtt tcttgatttt tgtaaagatg agcttgacga tggggtagaa tttctccatt
     2941 ggccatttgc actctggcca aaggttctta aatcgcctga gccaagaaaa ctgtctgagg
     3001 aaggattatc tgagtaatag cttggattat aagctgcact tcttgtggaa catgtaaaag
     3061 atcggtccaa atgtccttgc ctggaagata aaatctgctg gtcagaaagc gaaagctggt
     3121 cctctggaaa acctgtttgt ggcaggaggt gggttttgaa tacggagttg tggttcacta
     3181 aagtcacttc tccagcatcc atcctcatcc tatcagcaat atcatccata agatctatcg
     3241 atgaattcgc ggccgcaagc ttgtcgtcat cgtctttgta gtccatggta gatcaattct
     3301 gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     3361 tttgcgtcaa cggggcgggg ttattacgac attttggaaa gtcccgttga ttttggtgcc
     3421 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     3481 gctatccacg cccattggtg tactgccaaa accgcatcac catggtaata gcgatgacta
     3541 atacgtagat gtactgccaa gtaggaaagt cccgtaaggt catgtactgg gcataatgcc
     3601 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgg acttggcata tgatacactt
     3661 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     3721 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     3781 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     3841 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     3901 aacatggcgg tcatattgga catgagccaa tataaatgta catattatga tatagataca
     3961 acgtatgcaa tggccaatag ccaatattga tttatgctat ataaccaatg actaatatgg
     4021 ctaattgcca atattgattc aatgtataga tcagcttggc actggccgtc gttttacaac
     4081 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     4141 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     4201 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4261 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4321 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4381 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4441 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4501 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4561 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4621 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4681 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4741 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4801 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4861 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4921 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4981 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5041 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5101 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5161 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5221 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5281 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5341 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5401 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5461 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5521 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5581 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5641 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5701 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5761 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5821 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5881 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5941 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6001 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6061 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6601 ggaag
//