LOCUS       GFP-MAP1B               12115 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    GFP-MAP1B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR
  TITLE     Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B
            regulate microtubule dynamics in growing axons.
  JOURNAL   J Cell Sci. 2009 Jul 15;122(Pt 14):2424-35. doi: 10.1242/jcs.040162.
            Epub 2009 Jun 23.
  PUBMED    19549690
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             620..1336
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             620..1336
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(665..686)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(926..945)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1273..1294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8910..9031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8947..8966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9001..9020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9038..9493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9175..9196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9387..9406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9520..9624
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9626..9983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(9649..9669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9834..9969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9896..9915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10018..10812
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10072..10091)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10682..10701
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11000..11019)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11044..11091
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11420..12008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11909..11928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      781 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1021 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1081 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1141 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1201 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1321 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     1381 catggcgacc gtggtggtgg aagccaccga accggagcca tcgggcagca tcggcaaccc
     1441 ggcggcgtcc acctcgccca gcctgtcgca ccgcttccta gacagcaagt tctacttgct
     1501 ggtggtggtc ggcgagaccg taaccgaaga gcacctgagg cgtgccatcg gcaacatcga
     1561 gctggggatc cgatcgtggg acacaaacct gattgagtgc aacttggacc aggaactcaa
     1621 acttttcgta tctcgacact ctgcaagatt ctctcctgaa gttccaggac aaaagattct
     1681 tcatcaccga agtgacgtct tagaaacagt cgttctgatc aacccttcag atgaagccgt
     1741 cagcactgag gtgcgtttga tgatcactga cgctgcccgc cataaactgc tggtgctcac
     1801 cggacagtgc tttgagaaca ctggcgagct catcctgcag tctggctctt tctccttcca
     1861 gaacttcata gagattttca cagatcaaga gattggtgag ctcctaagca ccacccaccc
     1921 tgccaacaaa gccagcctca ccctcttctg tcccgaggag ggagactgga agaactccaa
     1981 ccttgacaga cacaatctcc aagacttcat caacattaag ctcaactcag cttctatctt
     2041 gccagaaatg gagggacttt ctgagttcac cgagtatctc tcagagtctg tagaagtccc
     2101 ctctcccttt gacatcctgg aacccccaac ctcgggcggc tttctgaagc tctccaagcc
     2161 ttgctgttac attttcccag ggggccgtgg ggactcggcc ctgtttgcag tgaacggatt
     2221 caacatgctc atcaacggag gatcagagag aaagtcttgc ttctggaagc tcatccggca
     2281 cttggaccgt gtggactcca tcctgctcac ccacattggg gatgacaatt tgcctgggat
     2341 caatagcatg ctgcagcgca agattgcaga gctggaagag gagcggtccc aggggtccac
     2401 cagcaacagt gactggatga aaaacctcat ctcccctgac ctgggggttg tgtttctcaa
     2461 cgtacctgaa aacctgaaag acccagaacc caacatcaag atgaagagaa gcatagaaga
     2521 agcatgcttc actctccagt acttaaacaa actgtccatg aaaccagagc ctctatttag
     2581 aagtgtaggc aataccattg agcctgtcat cctgttccag aagatgggag tgggcaagct
     2641 tgagatgtac gtgcttaacc cagtcaaaag cagcaaggaa atgcagtatt ttatgcaaca
     2701 gtggactggc accaacaaag acaaggccga actaatcctg cccaatggtc aagaagtaga
     2761 catcccgatt tcctacttaa cctcagtctc atctttgatt gtgtggcatc cggccaaccc
     2821 cgctgagaaa atcattcggg ttctgtttcc cggaaacagt acccagtaca acatcctaga
     2881 aggactggag aaactcaaac atctagattt cctaaagcag ccactggcca cccaaaaaga
     2941 tctcactggc caggtgccca cccccccagt gaaacaggtc aaactgaaac agcgggctga
     3001 cagccgagag agtctgaagc cagccacgaa accagttgcc agtaaatctg tgaggaagga
     3061 gtccaaggag gaaacccctg aagtcacaaa gactagtcaa gtggagaaaa cacccaaagt
     3121 tgaaagcaaa gagaaagtgt tagtgaaaaa agacaagcct gtaaagacag aaagtaaacc
     3181 ttcagtgaca gaaaaggagg tgtccagcaa ggaagagcag tccccagtga aagccgaggt
     3241 cgctgagaag caagccactg agagcaaacc caaagtcacc aaggacaaag tggtaaaaaa
     3301 ggaaataaag acaaaactcg aggaaaagaa agaggagaag cccaagaaag aagtggttaa
     3361 gaaggaagac aaaacacccc tcaagaaaga cgagaagcca agaaaggaag aggtgaagaa
     3421 ggagatcaag aaagaaatca aaaaagaaga gagaaaggag ctgaagaaag aggtgaagaa
     3481 ggaaacaccc ctgaaggatg ccaagaagga ggtgaagaaa gaggagaaga aagaagttaa
     3541 aaaggaagag aaggaaccca aaaaggaaat caagaagatc tccaaggaca taaagaaatc
     3601 aactcctctg tcagacacaa agaagccatc tgcgttaaaa ccaaaagtag cgaagaaaga
     3661 agagtccact aagaaggagc ctcttgctgc tgggaaactc aaggacaagg ggaaagtcaa
     3721 agtcattaaa aaggaaggca agaccacaga agcagctgcc acagctgttg gcactgctgc
     3781 caccacagct gccgtggtgg ctgcagctgg aatagcggcc agtggtcctg tcaaagaact
     3841 ggaagctgag aggtccctta tgtcctcccc tgaggatcta accaaggact ttgaggagct
     3901 aaaggctgag gagattgatg tggccaagga catcaagcct cagctggagc tcattgaaga
     3961 tgaagagaaa ctgaaggaaa cccagccggg agaagcctac gtcatccaga aagagacaga
     4021 agtcagcaaa ggttccgctg agtcacccga tgagggaatc accaccaccg agggggaggg
     4081 cgagtgtgag caaacccctg aggagctgga gccagttgag aagcagggcg tggatgacat
     4141 tgagaagttt gaagatgaag gagctggttt tgaagaatcc tcagagaccg gagactacga
     4201 agagaaggcg gaaactgagg aggctgagga accggaagag gacggggaag acaatgcgag
     4261 cgggagtgcc tctaagcaca gccccacaga agatgacgaa agcgcaaagg ctgaggcgga
     4321 cgtgcacctc aaggagaaga gggagtctgt ggtcagtggt gacgaccggg cggaagaaga
     4381 catggatgat gtgcttgaga aaggagaagc tgaacagtct gaggaagagg gcgaagagga
     4441 ggacaaagca gaggatgcca gagaggaagg ctatgagccc gacaaaactg aggctgagga
     4501 ttatgtgatg gccgtggctg acaaggcggc tgaggctgga gtcacagagg agcagtacgg
     4561 atacctgggc acgtcggcca agcaacctgg aatccagtct cctagccgag aacctgcatc
     4621 ttcaattcat gatgagaccc tacctggagg ctctgagagc gaggccactg cttcggatga
     4681 ggagaatagg gaagaccagc ctgaggaatt cactgctacc tctggatata ctcagtctac
     4741 cattgagata tctagtgagc ctacgccaat ggatgagatg tctactcctc gtgatgtgat
     4801 gagcgacgag accaacaacg aggagacaga gtccccatct caggagttcg taaacattac
     4861 caaatatgag tcctcgctgt attctcagga gtactccaaa cctgctgttg catcattcaa
     4921 tggattgtca gaagggtcaa aaacagacgc cactgatggt aaggattaca acgcttccgc
     4981 ctccaccata tcaccacctt cgtccatgga agaagacaaa ttcagcaagt ctgctcttcg
     5041 tgatgcttac tgctcagaag agaaagagtt gaaagccagt gccgagttag acatcaaaga
     5101 tgtttcagat gagagactta gcccagccaa gagtccatcc ctgagtcctt ctccgccatc
     5161 ccccatagag aagactcccc tgggtgaacg tagcgtgaat ttctctctga cacccaatga
     5221 gattaaagtg tcagcagagg gagaagcaag gtcagtgtcc cctggagtga cccaggcagt
     5281 agttgaagaa cattgtgcca gtcctgagga gaagaccctg gaagtagtgt cgccatccca
     5341 gtctgtgaca ggcagtgctg gccacacacc ttactaccaa tctcccaccg atgagaagtc
     5401 cagtcaccta cccacagaag tcactgaaaa gccacaggca gttccagtga gctttgaatt
     5461 cagtgaggcc aaagatgaga acgagagagc gtccctaagc cccatggatg agcctgtgcc
     5521 tgactcagag tctcctgtgg agaaggttct gtctccttta cgcagtcctc cccttcttgg
     5581 atctgagtcc ccatatgaag acttcctgag tgctgatagc aaggttcttg gcagacgttc
     5641 cgaaagcccc tttgaaggga agaatggaaa gcagggcttc ccagacagag aaagcccagt
     5701 ttctgacctg acttccactg gtctttacca agacaaacag gaagagaaaa gcacgggctt
     5761 catacccata aaggaagact ttggtccaga aaagaaaacc agtgatgttg aaaccatgag
     5821 ttctcaatca gctctagctt tggatgaaag gaaattggga ggagatgtat ctccaacaca
     5881 aatagacgtc agtcagtttg gctctttcaa agaagacacc aagatgtcca tttcagaagg
     5941 cactgtttca gacaagtcag ccacacctgt ggatgagggt gtggcagaag acacctactc
     6001 ccacatggag ggtgtggcct ccgtctctac tgcctctgtg gctacaagtt cgtttccaga
     6061 gccaaccaca gatgacgtgt ctccttctct gcatgccgag gtgggttctc cacattccac
     6121 agaagttgat gactccctgt ccgtttctgt ggtgcaaaca cctaccacgt tccaggaaac
     6181 agaaatgtct ccatctaaag aagagtgccc aagaccgatg tcaatttctc ctccagactt
     6241 ctcccccaag acagccaaat ccaggacacc agttcaagat caccgatctg aacagtcttc
     6301 tatgtctatt gagttcggcc aggaatcccc tgagcattct tttgctatgg actttagtcg
     6361 gcagtctcca gatcacccta ctctgggtgc cagtgtgctt catatcactg aaaacgggcc
     6421 aactgaagtg gactatagtc cctctgatat ccaggactct agtttgtcgc ataagatccc
     6481 gccaacggaa gagccatcct acacccagga taatgatctg tctgagctca tctctgtgtc
     6541 tcaggtagag gcttccccgt ccacctcttc agctcacacc ccttctcaga tagcctctcc
     6601 tcttcaggaa gacactctct ctgatgttgt tcctcccaga gagatgtcct tatatgcctc
     6661 acttgcgtct gaaaaagtgc agagcctgga aggagagaag ctctctccga aatctgatat
     6721 ttctccactc accccacgag agtcctcacc tttatattca cctggctttt cagattctac
     6781 ctctgcagct aaagagactg cagcagctca ccaagcctcc tcttccccac caatagatgc
     6841 agcaacagca gagccctatg gcttccgctc ctcaatgcta tttgatacaa tgcaacatca
     6901 tctggccttg aatagagatt tgactacatc tagtgtggag aaagacagcg gagggaagac
     6961 acccggagac tttaactatg cctatcaaaa gcctgagaat gccgccggat ctccagatga
     7021 agaagattat gactatgaat ctcaagagaa aaccatccgg acccacgacg tgggtggtta
     7081 ctactatgag aagacagaga gaaccataaa atccccatgt gacagtgggt actcctatga
     7141 gaccattgag aagaccacca agaccccaga agatggtggc tacacctgtg aaattaccga
     7201 gaaaaccact cggaccccag aagagggtgg ctattcatat gagatcagcg agaagacgac
     7261 acggacccct gaagtaagcg gctacaccta tgaaaagact gagaggtcca gaaggctcct
     7321 ggatgacatt agcaatggct atgatgacac tgaggatggc ggccacacac ttggcgactg
     7381 tagctactcc tatgaaacca ctgagaaaat taccagcttt cctgagtctg aaagctattc
     7441 ctacgagaca agtacaaaaa caacacggag tccagacacc tctgcatact gttacgagac
     7501 catggagaag atcaccaaga ccccacaggc atccacatac tcctatgaga cctcagaccg
     7561 atgctacact acggaaaaga agtccccctc agaggcacgc caggatgttg atttatgtct
     7621 ggtgtcctcc tgtgaattca agcatcccaa gacggagctc tcgccttcct tcattaatcc
     7681 aaatcctctt gagtggtttg ctggtgaaga gcccactgaa gaatcagaga agcccctcac
     7741 tcagtctgga ggagcccccc caccttcagg aggaaaacaa cagggcaggc aatgcgatga
     7801 aacgccaccc acctcagtca gtgagtcagc cccatcccag acagactctg atgttccccc
     7861 agagacggaa gagtgcccct ccatcacagc cgacgccaat attgactctg aagatgagtc
     7921 agaaactatc cccacagaca aaaccgtcac atacaaacac atggacccac ctccagcccc
     7981 catgcaagac cgaagccctt ctcctcgcca ccccgacgtg tccatggtgg acccagacgc
     8041 cttggcagtt gatcagaacc taggcaaggc tctgaaaaaa gacctgaagg agaagaccaa
     8101 gaccaagaag ccaggcacaa agaccaagtc ctcttcacct gtcaaaaagg gtgatgggaa
     8161 gtccaagcct ttagcagctt cccccaaacc aggagctttg aaggaatcct ccgacaaggt
     8221 gtccagagtg gcttctccca agaagaaaga atccgtggaa aaagctacaa agaccaccac
     8281 cactcctgag gtcaaagcca cacgcggcga agagaaggac aaggagacca agaacgcagc
     8341 taacgcctct gcatccaaat cagcgaagac cgcaacgaca ggaccaggaa ccactaagac
     8401 agccaagtcg tccaccgtgc ctccaggcct ccctgtgtat ttggacctgt gctatattcc
     8461 caaccacagc aacagtaaga atgtcgatgt tgagttcttc aagagagtga ggtcatctta
     8521 ctacgtggtg agtgggaacg accctgccgc ggaggagccc agccgggctg tcctggatgc
     8581 cttgttggaa gggaaggctc agtggggaag caacatgcag gtgactctga ttccgacaca
     8641 tgactccgag gtgatgaggg agtggtacca ggagacccac gagaagcagc aggacctcaa
     8701 catcatggtc ttagcaagca gcagtacagt ggtcatgcaa gacgagtcct tccccgcatg
     8761 caagatagaa ctgtagcccg ggatccaccg gatctagata actgatcata atcagccata
     8821 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8881 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9001 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     9061 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     9121 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     9181 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     9241 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     9301 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     9361 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     9421 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     9481 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9601 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     9661 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9721 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9781 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9841 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9901 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9961 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
    10021 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
    10081 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
    10141 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
    10201 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
    10261 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    10321 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    10381 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    10441 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    10501 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
    10561 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    10621 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    10681 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    10741 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    10801 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
    10861 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10921 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10981 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
    11041 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
    11101 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    11161 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    11221 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
    11281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11581 cgtagttagg ccaccacttc aagaactctg tatcaccgcc tacatacctc gctctgctaa
    11641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgc
//