| 質粒名稱: | PSD95-TS:YFP |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 42225 |
| 存儲實驗室: | Roger Tsien |
|
載體骨架基本信息 |
|
| 載體名稱: | pCAGGS |
| 載體出品公司: | -- |
| 空載體大小: | -- |
| 完整質粒大小: | 9947 bp |
| 載體修飾: | -- |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | -- |
| 載體抗性: | 氨芐霉素,100 μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | PSD95 |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子: | -- |
| 標簽/融合蛋白: | TS:YFP（插入件上的C端子） |
| 克隆方法: | -- |
| 5’克隆位點: | -- |
| 3’克隆位點: | -- |
| 5’測序引物 | -- |
| 3’測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | PSD95-TS:YFP was a gift from Roger Tsien (Addgene plasmid # 42225 ; //n2t.net/addgene:42225 ; RRID:Addgene_42225) |
| 參考文獻部分: | Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy. Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, Ellisman MH, Tsien RY, Lin MZ. Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 2012 Oct 28. 10.1038/nn.3246 PubMed 23103964 |
買家導航
PSD95-TS:YFP載體質粒基本信息
PSD95-TS:YFP質粒圖譜載體圖譜和PSD95-TS:YFP載體序列質粒序列多克隆位點信息
PSD95-TS:YFP質粒載體簡介
PSD95-TS:YFP質粒序列載體序列
LOCUS PSD95-TS:YFP 9947 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS PSD95-TS:YFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9947)
AUTHORS Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR,
Ellisman MH, Tsien RY, Lin MZ
TITLE Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates
in light and electron microscopy.
JOURNAL Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub
2012 Oct 28.
PUBMED 23103964
REFERENCE 2 (bases 1 to 9947)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..9947
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
CDS 3936..4400
/codon_start=1
/product="improved N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN155(I152L)
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
primer_bind complement(3981..4002)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4242..4261)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
CDS 5259..5285
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind 5483..5504
/label=EGFP-C
/note="EGFP, forward primer"
CDS 5547..5573
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(6971..6990)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 7036..7091
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(7090..7109)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(7450..7466)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7450..7466)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7463..7485)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7474..7490
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7498..7528)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7543..7564
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7623..7818
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 7669..7804
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7731..7750
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 7824..7958
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7861..7880)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7915..7934
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8026..8043)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8197..8785)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8277..8296)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8956..9816)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9579..9598
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9817..9921)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccggact ctagcctagg
1741 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct
1801 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca
1861 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gtgaacggaa
1921 cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct
1981 tcagcatcgc aggtggcacc gacaacccac acatcggtga cgacccatcc atctttatca
2041 ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc aacgacagca
2101 tcctgtttgt caatgaagtg gatgtccggg aggtgaccca ttcagctgca gtggaggccc
2161 tcaaagaggc gggttccatc gttcgcctct acgtcatgcg ccggaaaccc ccagctgaga
2221 agatcataga gatcaagctt atcaaagggc ctaaaggact tggcttcagc atcgcagggg
2281 gcgttgggaa ccagcacatc cctggagata atagcatcta cgtaaccaag atcatcgaag
2341 gaggcgctgc ccacaaggat ggcaggttgc agatcggaga caagatcctg gcggtcaaca
2401 gtgtggggct agaggatgtc atgcatgagg acgccgtggc agccctgaag aacacatatg
2461 acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc
2521 ccccagacat cacaacctca tattctcagc acctggacaa tgagatcagt catagcagct
2581 acttgggcac cgactacccc acagccatga cccccacttc ccctcggcgc tactcccccg
2641 tggccaagga cctgctaggg gaggaagata ttccccggga accaaggcgg atcgtgatcc
2701 atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggacggt gaaggcatct
2761 tcatctcctt catccttgct gggggtccag ctgacctcag tggggagcta cggaaggggg
2821 accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcatgaa caggctgcca
2881 ttgccctgaa gaacgcgggt cagacggtca cgatcatcgc tcagtataaa ccagaagagt
2941 atagccgatt cgaggccaag atccatgatc ttcgggaaca gcttatgaat agtagcctgg
3001 gctcagggac tgcatctctg cgaagcaacc ccaagcgggg cttctatatc agggccctgt
3061 ttgactacga caagaccaag gactgcggtt tcttgagcca ggccctgagc ttccactttg
3121 gggatgtgct tcatgtaatt gacgccagcg acgaagagtg gtggcaagcg cggcgggtcc
3181 actctgacag tgagaccgat gacattggct tcattcccag caaacggcgg gtcgagcgac
3241 gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca cagggtcgag
3301 aagactcggt tctgagctat gagacggtga cgcagatgga agtgcactac gctcgcccca
3361 tcatcatcct tgggcctacc aaagaccgtg ccaacgatga tcttctctcc gagttccccg
3421 acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat gagatagacg
3481 gccgcgatta ccactttgtc tcctcccggg agaaaatgga gaaggacatt caggcgcaca
3541 agttcattga ggctggccag tacaacagcc acctctacgg gaccagcgtc cagtctgtgc
3601 gagaggtagc agagcagggg aagcactgca tccttgatgt ctcagccaat gccgtgcggc
3661 ggctgcaggc ggcccacctg caccctatcg ccatcttcat ccgtccccgc tccctggaga
3721 atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc ttcgacaggg
3781 ccacgaagct ggagcaggag ttcacggagt gcttctcagc catcgtagag ggcgacagct
3841 ttgaagagat ctatcacaaa gtgaaacgtg tcatcgaaga cctctcaggc ccctacatct
3901 gggtcccagc ccgagagaga ctggagcccg ggcagatggt gagcaagggc gaggagctgt
3961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
4021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
4081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
4141 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
4201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
4261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
4321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
4381 acaacgtcta tatcaccgcc gacaagcagt tcgaaaccgg gcccatggcc agcatgactg
4441 gtggacagca aatggggtcg acggaggacg tggtgtgctg ccactcaatc tacggcaaga
4501 agaagggtga tatcgacacc taccgataca taggctcttc cgggacaggc tgcgtggtca
4561 tagtgggcag gatcgtcttg tccggatccg gcactagtgc gcccatcacg gcgtacgccc
4621 agcagacgag aggcctccta gggtgtataa tcaccagcct gactggccgg gacaaaaacc
4681 aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca
4741 tcaatggggt atgctgggca gtctaccacg gggccggaac gaggaccatc gcatcaccca
4801 agggtcctgt catccagatg tataccaatg tggaccaaga ccttgtgggc tggcccgctc
4861 ctcaaggttc ccgctcattg acaccctgta cctgcggctc ctcggacctt tacctggtca
4921 cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt
4981 cgccccggcc catttcctac ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg
5041 gacacgccgt gggcctattc agggccgcgg tgtgcacccg tggagtggct aaagcggtgg
5101 actttatccc tgtggagaac ctagagacaa ccatgagatc cccggtgttc acggacaact
5161 cctctccacc agcagtcacc ctgacgcacc caatcaccaa aatcgatagg gaggttctct
5221 accaggagtt cgatgagatg gaagagtgct ctcagcacta tccctacgat gtgcccgatt
5281 acgctggcgc gtctgcatgc cagaagaacg gcatcaaggc caacttcaag atccgccaca
5341 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
5401 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
5461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
5521 ctctcggcat ggacgagctg tacaagtatc cctacgatgt gcccgattac gctggcgcgc
5581 cttgattcct gccctggctt ggcctggact caccctgcct ccaccacctg ggcccttggt
5641 ctggactgaa tagcccaagc ccttcgtacc ctagcctttc ttcccaccct tcttatttat
5701 ttcctttctg actggatcga gcccattgga gggaggacac tcctctctgc atgtatccct
5761 gcaccccaga tcagggcccc cagaccccag gaacctgggg tctggggggg agctgggctc
5821 ctggttccga gcccttgctc cttaagactc ctacccctgc ccgcccccat gcacacacag
5881 acccactggg ggcctctgcc ctccccattt tctcccacac acattccaga agtcagggcc
5941 cctctcaagg agtacccgct gtagggatgc agggccacag gcttccgcgc tctcccgagg
6001 caggggctgg ggtcacccct gccccatcat aactccccac gccgtttgag ttctccttta
6061 ttttctccat tcttttcttc tcaaaggtgg tttttggggg gagaagcagg ggggctctcc
6121 tgagggtccc cccgtctttc acacacctcc cacctttttt ctttgccggt ttgcatgagt
6181 ggaaggtcta actgtggctt tttttttttt ctgggatttt atttcatttt atttgatttt
6241 atttttttgg gaaaagggga gggatgggtc tggggagtgg ggaatgtggg aggggggtgg
6301 gggggcaaag ggtcaggggt tgggtgtccg ggagccaggg gaggacagga aatgctgccg
6361 ccttctgcaa tttatttatt tttttctttt gagagagtga aaggaagaga cagacacttg
6421 aaaccccgtg tgtggcctgg ttctttgggg tgagggaggg aagtggcctg ggatgggtgg
6481 ctctgagaac ttcagatcct tccttacctc acagagtata gggtgcctaa aggggtcatg
6541 ggaaggactt accagaccga ggcagacaga gctgccaccg ccgggcctgg gaagagccgg
6601 agctgggtta gcccctggca gactcgccat tgatccctgc aagctcagtc gagccctgac
6661 cactccccct agctggcctc atccctggtg acagcacatt catccctaaa gagaaataca
6721 gaggctcctc ggcttacagt ggagtgaaga ggacctatgt ataaaactac ccaggatgta
6781 caaattcaat acacctaacc ggccaaacat cagggctcgg ctccacccat ccttaaaggc
6841 tcagcacaca ggcccgcggt tgggcaaagc ctgttgtgtc acaaagcact gaacagctcg
6901 tgtgacggcc tgggagctga cgctactgct gctcagcatc aagtgccacc caatgcggcc
6961 gctcgacgga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
7021 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
7081 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
7141 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta
7201 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
7261 gccttgactt gaggttagat ttttttttat attttgtttg tgttattttt ttctttaaca
7321 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
7381 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc
7441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
7501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
7561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
7621 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
7741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
7801 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
7861 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
7921 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
7981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
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