LOCUS       SFG.wtCNa_opt.IRES.eGFP               9244 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    SFG.wtCNa_opt.IRES.eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Brewin J, Mancao C, Straathof K, Karlsson H, Samarasinghe S, Amrolia
            PJ, Pule M
  TITLE     Generation of EBV-specific cytotoxic T-cells that are resistant to 
            calcineurin inhibitors for the treatment of post-transplant 
            lymphoproliferative disease.
  JOURNAL   Blood. 2009 Sep 21. ():.
  PUBMED    19770360
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    655..1012
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1423..1445
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1461..1477
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1503..1877
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1815..1831
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    3513..4064
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3657..3674)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3884..3903
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4064..4783
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4064..4780
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4109..4130)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4370..4389)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4717..4738
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5049..5518
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6217..6234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6217..6233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6226..6248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6442..6461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6561..6583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6622..6640)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6708..6812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6813..7673
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7031..7050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7844..8432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8333..8352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8586..8603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8720..8741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8756..8786
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8794..8810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8799..8821
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8818..8834
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8818..8834
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             9243..592
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttatgctccc cgagctcaat
      421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      841 tggcccgacc tgagtcctaa aatcccgatc gtttaggact ctttggtgca ccccccttag
      901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggct
     1081 agcctgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1141 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1201 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1261 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtggggtaca
     1321 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1381 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1441 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1501 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1561 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1621 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1681 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1741 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1801 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1861 gaccatcctc tagactgcca ccatgagcga gcccaaggcc atcgacccca agctgtccac
     1921 caccgacaga gtggtgaagg ccgtgccctt tcccccctcc caccgcctga ccgccaagga
     1981 ggtgttcgac aacgatggca agcccagagt ggacatcctg aaagcccacc tgatgaagga
     2041 gggcagactg gaggagagcg tggccctgcg catcatcacc gagggcgcca gcatcctgcg
     2101 ccaggagaag aacctgctgg atatcgacgc ccctgtgacc gtgtgcggcg acatccacgg
     2161 acagtttttc gacctgatga agctgttcga ggtgggcggc tctcccgcca acaccagata
     2221 cctgttcctg ggcgactacg tggacagagg ctacttctct atcgagtgcg tgctgtacct
     2281 gtgggccctg aagatcctgt accccaagac cctgttcctg ctgagaggca accacgagtg
     2341 ccgccacctg accgagtact ttaccttcaa gcaggagtgc aagatcaagt acagcgagcg
     2401 cgtgtacgat gcctgcatgg acgccttcga ctgtctgccc ctggccgctc tgatgaacca
     2461 gcagttcctg tgtgtgcacg gaggcctgag ccccgagatc aacaccctgg acgacatccg
     2521 gaagctggac cgcttcaagg agccccctgc ctatggcccc atgtgcgaca tcctgtggag
     2581 cgaccccctg gaggactttg gcaacgagaa gacccaggag cactttaccc acaacaccgt
     2641 gagaggctgc agctacttct actcctaccc tgctgtgtgc gagttcctgc agcacaacaa
     2701 cctgctgagc atcctgaggg cccacgaggc ccaggatgcc ggctaccgca tgtaccgcaa
     2761 gtcccagacc accggcttcc cctccctgat caccatcttc tctgccccca actacctgga
     2821 cgtgtacaac aacaaagccg ctgtgctgaa gtacgagaac aacgtgatga acatccggca
     2881 gttcaactgc tccccccacc cctactggct gcccaacttc atggacgtgt tcacctggtc
     2941 cctgcccttt gtgggcgaga aggtgaccga gatgctggtg aacgtgctga acatctgctc
     3001 cgacgatgag ctgggcagcg aggaggacgg cttcgatggc gccaccgctg ccgccagaaa
     3061 ggaggtgatc cgcaataaga tcagagccat cggcaagatg gccagagtgt tctccgtgct
     3121 gcgcgaggag agcgagtccg tgctgaccct gaagggcctg acccccaccg gcatgctgcc
     3181 ctccggcgtg ctgagcggag gcaagcagac cctgcagagc gccacagtgg aggccatcga
     3241 ggccgatgag gccatcaagg gcttctcccc ccagcacaag atcaccagct tcgaggaggc
     3301 caagggcctg gaccggatca acgagcggat gccacccaga cgggatgcca tgcccagcga
     3361 tgccaacctg aactccatca acaaggccct gaccagcgag accaacggca ccgacagcaa
     3421 cggcagcaac tcctccaaca tccagtgaac gcgtacgtcg cgaccgcgga catgtacaga
     3481 gctcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc gcttggaata
     3541 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3601 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3661 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3721 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     3781 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3841 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3901 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3961 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     4021 aaccacgggg acgtggtttt cctttgaaaa acacgataat aatatggtga gcaagggcga
     4081 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4141 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4201 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4261 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4321 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4381 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4441 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4501 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4561 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4621 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4681 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4741 cgccgccggg atcactctcg gcatggacga gctgtacaag taatccggat tagtccaatt
     4801 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
     4861 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
     4921 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     4981 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     5041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5101 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     5161 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     5221 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     5281 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     5341 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     5401 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     5461 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     5521 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     5581 atagtactta aagttacatt ggcttccttg aaataaacat ggagtattca gaatgtgtca
     5641 taaatatttc taattttaag atagtatctc cattggcttt ctactttttc ttttattttt
     5701 ttttgtcctc tgtcttccat ttgttgttgt tgttgtttgt ttgtttgttt gttggttggt
     5761 tggttaattt ttttttaaag atcctacact atagttcaag ctagactatt agctactctg
     5821 taacccaggg tgaccttgaa gtcatgggta gcctgctgtt ttagccttcc cacatctaag
     5881 attacaggta tgagctatca tttttggtat attgattgat tgattgattg atgtgtgtgt
     5941 gtgtgattgt gtttgtgtgt gtgactgtga aaatgtgtgt atgggtgtgt gtgaatgtgt
     6001 gtatgtatgt gtgtgtgtga gtgtgtgtgt gtgtgtgtgc atgtgtgtgt gtgtgactgt
     6061 gtctatgtgt atgactgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     6121 gttgtgaaaa aatattctat ggtagtgaga gccaacgctc cggctcaggt gtcaggttgg
     6181 tttttgagac agagtctttc acttagcttg gaattcactg gccgtcgttt tacaacgtcg
     6241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     6301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6361 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6421 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6481 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     6541 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     6601 gaaacgcgcg atgacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6661 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6721 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6781 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6841 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6901 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6961 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7021 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7081 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7141 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7201 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7261 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7321 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7381 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7441 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7501 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7561 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7621 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7681 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7741 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7801 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7861 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7921 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7981 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8041 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8101 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8161 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8221 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8281 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8341 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8401 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8461 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8521 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8581 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8641 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8701 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8761 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8821 gaaacagcta tgaccatgat tacgccaagc tttgctctta ggagtttcct aatacatccc
     8881 aaactcaaat atataaagca tttgacttgt tctatgccct agggggcggg gggaagctaa
     8941 gccagctttt tttaacattt aaaatgttaa ttccatttta aatgcacaga tgtttttatt
     9001 tcataagggt ttcaatgtgc atgaatgctg caatattcct gttaccaaag ctagtataaa
     9061 taaaaataga taaacgtgga aattacttag agtttctgtc attaacgttt ccttcctcag
     9121 ttgacaacat aaatgcgctg ctgagcaagc cagtttgcat ctgtcaggat caatttccca
     9181 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
     9241 tgaa
//