LOCUS       gRNA_DNMT3a-T1 Sequencing Result	3974 bp 	DNA	SYN
DEFINITION  gRNA_DNMT3a-T1 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="gRNA_DNMT3a-T1 Sequencing Result"
                     /mol_type="other DNA"
     promoter        143..172
                     /label="lac_promoter"
     misc_feature    186..208
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /label="M13_reverse_primer"
     misc_feature    239..256
                     /label="Sp6_primer"
     promoter        complement(481..737)
                     /label="hU6_promoter"
     misc_feature    complement(532..551)
                     /label="LKO1_5_primer"
     promoter        complement(862..880)
                     /label="T7_promoter"
     misc_feature    884..1027
                     /label="lacZ_a"
     promoter        complement(887..903)
                     /label="M13_forward20_primer"
     misc_feature    complement(896..918)
                     /label="M13_pUC_fwd_primer"
     misc_feature    1050..1343
                     /label="ccdB"
     promoter        1554..1603
                     /label="NEOKAN_promoter"
     CDS             1692..2486
                     /label="ORF frame 3"
     gene            1695..2483
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(2001..2534)
                     /label="ORF frame 1"
     promoter        2620..2648
                     /label="AmpR_promoter"
     gene            2693..3064
                     /label="bleo"
                     /gene="bleo"
     misc_feature    2693..3067
                     /label="sh_ble"
     rep_origin      3189..3808
                     /label="pBR322_origin"
ORIGIN
    1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
   61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
  121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
  181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
  241 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
  301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTTAAT GCCAACTTTG TACAAGAAAG
  361 CTGGGTCTAG AAAAAAAGCA CCGACTCGGT GCCACTTTTT CAAGTTGATA ACGGACTAGC
  421 CTTATTTTAA CTTGCTATTT CTAGCTCTAA AACCCTTGGG CCGCGCATCA TGCGGTGTTT
  481 CGTCCTTTCC ACAAGATATA TAAAGCCAAG AAATCGAAAT ACTTTCAAGT TACGGTAAGC
  541 ATATGATAGT CCATTTTAAA ACATAATTTT AAAACTGCAA ACTACCCAAG AAATTATTAC
  601 TTTCTACGTC ACGTATTTTG TACTAATATC TTTGTGTTTA CAGTCAAATT AATTCTAATT
  661 ATCTCTCTAA CAGCCTTGTA TCGTATATGC AAATATGAAG GAATCATGGG AAATAGGCCC
  721 TCTTCCTGCC CGACCTTGGT ACCGGATCCA GTCGACTGAA TTGGTTCCTT TAAAGCCTGC
  781 TTTTTTGTAC AAAGGGCGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG
  841 CATCTAGAGG GCCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT
  901 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC
  961 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT
 1021 TGCGCAGCCT ATACGTACGG CAGTTTAAGG TTTACACCTA TAAAAGAGAG AGCCGTTATC
 1081 GTCTGTTTGT GGATGTACAG AGTGATATTA TTGACACGCC GGGGCGACGG ATGGTGATCC
 1141 CCCTGGCCAG TGCACGTCTG CTGTCAGATA AAGTCTCCCG TGAACTTTAC CCGGTGGTGC
 1201 ATATCGGGGA TGAAAGCTGG CGCATGATGA CCACCGATAT GGCCAGTGTG CCGGTCTCCG
 1261 TTATCGGGGA AGAAGTGGCT GATCTCAGCC ACCGCGAAAA TGACATCAAA AACGCCATTA
 1321 ACCTGATGTT CTGGGGAATA TAAATGTCAG GCATGAGATT ATCAAAAAGG ATCTTCACCT
 1381 AGATCCTTTT CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA
 1441 GCTACTGGGC TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA
 1501 GTGGGCTTAC ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT
 1561 TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT
 1621 TCTCGCCGCC AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG
 1681 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
 1741 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
 1801 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
 1861 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
 1921 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
 1981 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
 2041 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG
 2101 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG
 2161 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA
 2221 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA
 2281 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
 2341 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG
 2401 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
 2461 ATCGCCTTCT TGACGAGTTC TTCTGAATTA TTAACGCTTA CAATTTCCTG ATGCGGTATT
 2521 TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACA GGTGGCACTT TTCGGGGAAA
 2581 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
 2641 GAGACAATAA CCCTGATAAA TGCTTCAATA ATAGCACGTG AGGAGGGCCA CCATGGCCAA
 2701 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
 2761 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
 2821 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
 2881 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
 2941 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
 3001 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
 3061 GGACTGACAC GTGCTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
 3121 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
 3181 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
 3241 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
 3301 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
 3361 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
 3421 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
 3481 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
 3541 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
 3601 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
 3661 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
 3721 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
 3781 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGGCT TTTGCTGGCC
 3841 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
 3901 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
 3961 CGAGGAAGCG GAAG
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