| 質粒名稱: | mEos3.2-N1 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 54525 |
| 存儲實驗室: | Michael Davidson, Tao Xu |
|
載體骨架基本信息 |
|
| 載體名稱: | mEos3.2-N1 |
| 載體出品公司: | -- |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | 4750 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | 新霉素（G418） |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | CMV |
| 標簽/融合蛋白: | mEos3.2（主干網上的C終端） |
| 克隆方法: | -- |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | CMV-F |
| 3' 測序引物 | SV40pA-R |
|
文獻引用方法 |
|
| 材料和方法部分: | mEos3.2-N1 was a gift from Michael Davidson & Tao Xu (Addgene plasmid # 54525 ; //n2t.net/addgene:54525 ; RRID:Addgene_54525) |
| 參考文獻部分: | Rational design of true monomeric and bright photoactivatable fluorescent proteins. Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, Xu T. Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021. 10.1038/nmeth.2021 PubMed 22581370 |
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mEos3.2-N1載體 mEos3.2N1質粒基本信息
mEos3.2-N1質粒圖譜 mEos3.2N1載體圖譜和mEos3.2-N1載體序列 mEos3.2N1質粒序列多克隆位點信息
mEos3.2-N1質粒 mEos3.2N1載體簡介
mEos3.2-N1質粒序列 mEos3.2N1載體序列
LOCUS mEos3.2-N1 4693 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission:
516 / 580.
ACCESSION .
VERSION .
KEYWORDS mEos3.2-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4693)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 4693)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..4693
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 598..678
/label=MCS
/note="multiple cloning site"
CDS 686..1366
/codon_start=1
/product="improved monomeric variant of green-to-red
photoswitchable fluorescent protein EosFP (Zhang et al.,
2012)"
/label=mEos3.2
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
polyA_signal 1488..1609
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1525..1544)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1579..1598
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1616..2071)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1753..1774)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1965..1984
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2098..2202
/gene="bla"
/label=AmpR promoter
promoter 2204..2561
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2227..2247)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2412..2547
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2474..2493
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2596..3390
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2650..2669)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3260..3279
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3578..3597)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3622..3669
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3998..4586
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4487..4506
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
661 ggcccgggat ccaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaaact
721 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
781 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt
841 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccaaatatcc
901 agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
961 cttgactttc gaagacgggg gcatttgcaa cgccagaaac gacataacaa tggaagggga
1021 cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat
1081 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
1141 gctgacgggt gatattgaga tggctttgtt gcttgaagga aatgcccatt accgatgtga
1201 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggcg cccactttgt
1261 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
1321 gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct
1381 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
1441 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
1501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
1561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
1621 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
1681 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
1741 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
1801 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
1861 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
1921 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
1981 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
2041 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
2101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
2161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
2221 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
2281 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
2341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
2401 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
2461 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
2521 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
2581 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
2641 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
2701 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
2761 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
2821 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
2881 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
2941 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
3001 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
3061 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
3121 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
3181 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
3241 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
3301 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
3361 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
3421 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
3481 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
3541 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
3601 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
3661 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
3721 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
3781 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
3841 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
3901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
3961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
4021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
4081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
4141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
4201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
4261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
4321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
4381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
4441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
4501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
4561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
4621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
4681 accgtattac cgc
//
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