LOCUS       pAC-85b               9424 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea 
            agglomerans) Eho10, and will produce beta-carotene in E. coli when 
            complemented with a gene encoding phytoene synthase.
ACCESSION   .
VERSION     .
KEYWORDS    pAC-85b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A portfolio of plasmids for identification and analysis of 
            carotenoid pathway enzymes: Adonis aestivalis as a case study.
  JOURNAL   Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
  PUBMED    17634749
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     133..152
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(219..321)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(713..730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(847..1392)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(927..946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1504..1532
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7111..7130)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(8983..218)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
       61 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
      121 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
      181 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
      241 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      301 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
      361 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
      421 tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg
      481 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
      541 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg
      601 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
      661 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
      721 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
      781 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
      841 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
      901 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa
     1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
     1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
     1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
     1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
     1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
     1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
     1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
     1861 cgtcctgtgg atctaaaggc acagcgtctc atgcttcgca caatgtaaaa ctgcttcaga
     1921 acctggcgag agctatccgc gcggtctacg gttaactgat actaaaagac aattcagcgg
     1981 gtaaccttgc aatggtgagt ggcagtaaag cgggcgtttc gcctcatcgc gaaatagaag
     2041 taatgagaca atccattgac gatcacctgg ctggcctgtt acctgaaacc gacagccagg
     2101 atatcgtcag ccttgcgatg cgtgaaggcg tcatggcacc cggtaaacgg atccgtccgc
     2161 tgctgatgct gctggccgcc cgcgacctcc gctaccaggg cagtatgcct acgctgctcg
     2221 atctcgcctg cgccgttgaa ctgacccata ccgcgtcgct gatgctcgac gacatgccct
     2281 gcatggacaa cgccgagctg cgccgcggtc agcccactac ccacaaaaaa tttggtgaga
     2341 gcgtggcgat ccttgcctcc gttgggctgc tctctaaagc ctttggtctg atcgccgcca
     2401 ccggcgatct gccgggggag aggcgtgccc aggcggtcaa cgagctctct accgccgtgg
     2461 gcgtgcaggg cctggtactg gggcagtttc gcgatcttaa cgatgccgcc ctcgaccgta
     2521 cccctgacgc tatcctcagc accaaccacc tcaagaccgg cattctgttc agcgcgatgc
     2581 tgcagatcgt cgccattgct tccgcctcgt cgccgagcac gcgagagacg ctgcacgcct
     2641 tcgccctcga cttcggccag gcgtttcaac tgctggacga tctgcgtgac gatcacccgg
     2701 aaaccggtaa agatcgcaat aaggacgcgg gaaaatcgac gctggtcaac cggctgggcg
     2761 cagacgcggc ccggcaaaag ctgcgcgagc atattgattc cgccgacaaa cacctcactt
     2821 ttgcctgtcc gcagggcggc gccatccgac agtttatgca tctgtggttt ggccatcacc
     2881 ttgccgactg gtcaccggtc atgaaaatcg cctgataccg cccttttggg ttcaagcagt
     2941 acataacgat ggaaccacat tacaggagta gtgatgaatg aaggacgagc gccttgttca
     3001 gcgtaagaac gatcatctgg atatcgttct cgacccccgt cgcgccgtaa ctcaggctag
     3061 cgcaggtttt gagcgctggc gctttaccca ctgcgccctg ccagagctga attttagcga
     3121 catcacgctg gaaaccacct tcctgaatcg gcagctacag gctccgctgc tgatcagctc
     3181 catgaccggc ggcgttgagc gctcgcgcca tatcaaccgc cacctcgccg aggcggcgca
     3241 ggtgctaaaa attgcgatgg gggtgggctc ccagcgcgtc gccattgaga gcgacgcggg
     3301 cttagggctg gataaaaccc tgcggcagct ggctccggac gtgccgctgc tggcgaacct
     3361 cggcgcggcg cagctgaccg gcagaaaagg tattgattac gcccgacggg ccgtggagat
     3421 gatcgaggcg gatgcgctga ttgtgcacct aaacccgctg caggaggcgc tacagcccgg
     3481 cggcgatcgc gactggcgcg gacggctggc ggctattgaa actctggtcc gcgagctgcc
     3541 cgttccgctg gtggtgaaag aggtgggagc cggtatctcc cgaaccgtgg ccgggcagct
     3601 gatcgatgcc ggcgttaccg tgattgacgt cgcgggcgcg ggcggcacca gctgggccgc
     3661 cgttgaaggc gagcgggcgg ccaccgagca gcagcgcagc gtggccaacg tctttgccga
     3721 ctgggggatc cccaccgctg aggcgctggt tgacattgcc gaggcctggc cgcagatgcc
     3781 ccttattgcc tcgggcggga ttaaaaacgg cgtcgaccag ccgcgctacg ttgccgaggc
     3841 taatctggtg atcacccacg gcggtctcaa taccgtactg gatgcgctgg ctgccgcgac
     3901 gccggtgctg gcggtgccac tctctttcga ccagcccgcc gtggctgccc ggctggtcta
     3961 taacgggctg ggtcgccggg tatcgcgctt tgccagacag cagacgctgg cggatgagat
     4021 tgcccaactg ctgggggatg agacgctgca tcagcgtctg gcgacggccc gccagcagct
     4081 taacgacgcc gggggcacgc cccgtgcggc gaccctgatt gaacaggcca tagcagggag
     4141 tgagagcgta tcgtgaggga tctgatttta gtcggcggcg gcctggccaa cgggctgatc
     4201 gcctggcgtc tgcgccagcg ctacccgcag cttaacctgc tgctgatcga ggccggggag
     4261 cagcccggcg ggaaccatac ctggtcattc catgaagacg atctgactcc cgggcagcac
     4321 gcctggctgg ccccgctggt ggcccacgcc tggccgggct atgaggtgca gtttcccgat
     4381 cttcgccgtc gcctcgcgcg cggctactac tccattacct cagagcgctt tgccgaggcc
     4441 ctgcatcagg cgctggggga gaacatctgg ctaaactgtt cggtgagcga ggtgttaccc
     4501 aatagcgtgc gccttgccaa cggtgaggcg ctgcttgccg gagcggtgat tgacggacgc
     4561 ggcgtgaccg ccagttcggc gatgcaaacc ggctatcagc tctttcttgg tcagcagtgg
     4621 cggctgacac agccccacgg cctgaccgta ccgatcctga tggatgccac ggtggcgcag
     4681 cagcagggct atcgctttgt ctacacgctg ccgctctccg ccgacacgct gctgatcgag
     4741 gatacgcgct acgccaatgt cccgcagcgt gatgataatg ccctacgcca gacggttacc
     4801 gactatgctc acagcaaagg gtggcagctg gcccagcttg aacgcgagga gaccggctgt
     4861 ctgccgatta ccctggcggg tgacatccag gctctgtggg ccgatgcgcc gggcgtgccg
     4921 cgctcgggaa tgcgggctgg gctatttcac cctaccactg gctattcgct gccgctggcg
     4981 gtggcccttg ccgacgcgat tgccgacagc ccgcggctgg gcagcgttcc gctctatcag
     5041 ctcacccggc agtttgccga acgccactgg cgcaggcagg gattcttccg cctgctgaac
     5101 cggatgcttt tcctggccgg gcgcgaggag aaccgctggc gggtgatgca gcgcttttat
     5161 gggctgccgg agcccaccgt agagcgcttt tacgccggtc ggctctctct ctttgataag
     5221 gcccgcattt tgacgggcaa gccaccggtt ccgctgggcg aagcctggcg ggcggcgctg
     5281 aaccattttc ctgacagacg agataaagga tgaaaaaaac cgttgtgatt ggcgcaggct
     5341 ttggtggcct ggcgctggcg attcgcctgc aggcggcagg gatcccaacc gtactgctgg
     5401 agcagcggga caagcccggc ggtcgggcct acgtctggca tgaccagggc tttacctttg
     5461 acgccgggcc gacggtgatc accgatccta ccgcgcttga ggcgctgttc accctggccg
     5521 gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa acccttctac cgactctgct
     5581 gggagtccgg gaagaccctc gactatgcta acgacagcgc cgagcttgag gcgcagatta
     5641 cccagttcaa cccccgcgac gtcgagggct accggcgctt tctggcttac tcccaggcgg
     5701 tattccagga gggatatttg cgcctcggca gcgtgccgtt cctctctttt cgcgacatgc
     5761 tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca gagcgtctac cagtcggttt
     5821 cgcgctttat tgaggatgag catctgcggc aggccttctc gttccactcc ctgctggtag
     5881 gcggcaaccc cttcaccacc tcgtccatct acaccctgat ccacgccctt gagcgggagt
     5941 ggggggtctg gttccctgag ggcggcaccg gggcgctggt gaacggcatg gtgaagctgt
     6001 ttaccgatct gggcggggag atcgaactca acgcccgggt cgaagagctg gtggtggccg
     6061 ataaccgcgt aagccaggtc cggctggcgg atggtcggat ctttgacacc gacgccgtag
     6121 cctcgaacgc tgacgtggtg aacacctata aaaagctgct cggccaccat ccggtggggc
     6181 agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa ctcgctgttt gtgctctact
     6241 tcggcctgaa ccagcctcat tcccagctgg cgcaccatac catctgtttt ggtccccgct
     6301 accgggagct gatcgacgag atctttaccg gcagcgcgct ggcggatgac ttctcgctct
     6361 acctgcactc gccctgcgtg accgatccct cgctcgcgcc tcccggctgc gccagcttct
     6421 acgtgctggc cccggtgccg catcttggca acgcgccgct ggactgggcg caggaggggc
     6481 cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg ctatatgccc ggcctgcgta
     6541 gccagctggt gacccagcgg atctttaccc cggcagactt ccacgacacg ctggatgcgc
     6601 atctgggatc ggccttctcc atcgagccgc tgctgaccca aagcgcctgg ttccgcccgc
     6661 acaaccgcga cagcgacatt gccaacctct acctggtggg cgcaggtact caccctgggg
     6721 cgggcattcc tggcgtagtg gcctcggcga aagccaccgc cagcctgatg attgaggatc
     6781 tgcaatgagc caaccgccgc tgcttgacca cgccacgcag accatggcca acggctcgaa
     6841 aagttttgcc accgctgcga agctgttcga cccggccacc cgccgtagcg tgctgatgct
     6901 ctacacctgg tgccgccact gcgatgacgt cattgacgac cagacccacg gcttcgccag
     6961 cgaggccgcg gcggaggagg aggccaccca gcgcctggcc cggctgcgca cgctgaccct
     7021 ggcggcgttt gaaggggccg agatgcagga tcctctacgc cggacgcatc gtggccggca
     7081 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     7141 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     7201 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     7261 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     7321 agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     7381 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     7441 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     7501 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     7561 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     7621 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     7681 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     7741 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     7801 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     7861 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     7921 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     7981 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     8041 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     8101 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     8161 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     8221 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     8281 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcccct acgtgctgct gaagttgccc
     8341 gcaacagaga gtggaaccaa ccggtgatac cacgatacta tgactgagag tcaacgccat
     8401 gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgtccg gtagctcctt
     8461 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     8521 actcgtagga caggtgccgg cagcgcccaa cagtcccccg gccacggggc ctgccaccat
     8581 acccacgccg aaacaagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     8641 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctaaccgt ttttatcagg
     8701 ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga
     8761 gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
     8821 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
     8881 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     8941 agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     9001 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     9061 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     9121 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     9181 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     9241 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     9301 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     9361 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     9421 acgg
//