| 質粒名稱: | pADH118 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 90983 |
| 存儲實驗室: | Aaron Hernday |
|
載體骨架基本信息 |
|
| 載體名稱: | pUC19 |
| 載體出品公司: | Clontech |
| 空載體大小: | 2242bp |
| 完整質粒大小: | 4150bp |
| 載體類型: | CRISPR |
| 篩選標記: | Nourseothricin (NAT) |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: |
NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
gRNA/shRNA sequencenone
|
|
文獻引用方法 |
|
| 材料和方法部分: | pADH118 was a gift from Aaron Hernday (Addgene plasmid # 90983 ; //n2t.net/addgene:90983 ; RRID:Addgene_90983) |
| 參考文獻部分: | An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD. mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr. 10.1128/mSphereDirect.00149-17 PubMed 28497115 |
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pADH118載體質粒基本信息
pADH118質粒圖譜載體圖譜和pADH118載體序列質粒序列多克隆位點信息
pADH118質粒載體簡介
pADH118質粒序列載體序列
LOCUS pADH118 4150 bp DNA circular SYN 14-APR-2025
DEFINITION NAT-marked ""empty"" gRNA expression construct; part 2 of 2 of C.alb
LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4150)
AUTHORS Nguyen N, Quail MMF, Hernday AD
TITLE An Efficient, Rapid, and Recyclable System for CRISPR-Mediated
Genome Editing in Candida albicans.
JOURNAL mSphere. 2017 Apr 26;2(2):e00149-17. doi:
10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
PUBMED 28497115
REFERENCE 2 (bases 1 to 4150)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date:
"2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT Sequence Label: pADH118
FEATURES Location/Qualifiers
source 1..4150
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 38..56
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 627..824
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
misc_RNA 1146..1221
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter complement(join(2042..2048,2049..2066,2067..2072))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2087..2108
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2225..2242)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2396..2984)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2476..2495)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3155..3946,3947..4015))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3778..3797
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4016..4120)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
61 tgacggcacg gccacgcgtt taaaccgcca ggtgatgctg aagctattga agctttggat
121 ggttctttca ctaccgatac tgtttttaga gttactgcta ctggtgatgg tttcactttg
181 agagaagttc cagttgatcc accattgact aaggttttcc cagatgatga atccgatgat
241 gaatccgatg ctggtgaaga tggtgatcca gattctagaa ctttcgttgc ttatggtgat
301 gatggtgatt tggctggttt cgttgttgtt tcttattctg gttggaacag aagattgact
361 gttgaagata ttgaagttgc tccagaacat agaggtcatg gtgttggtag agctttgatg
421 ggtttggcta ctgaattcgc cagagaaaga ggtgctggtc atttgtggtt ggaagttacc
481 aatgttaatg ctccagctat tcatgcttat agaagaatgg gtttcacttt gtgtggtttg
541 gatactgctt tatacgatgg tactgcttcc gatggtgaac aagctttgta tatgtctatg
601 ccatgtccat aataactcga gtcagtactg acaataaaaa gattcttgtt ttcaagaact
661 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
721 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
781 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
841 aacatctaat caactcccag atcaccacta tacatttaaa agtcattggt caataactat
901 actcgagtat tgcctcatca aagaaacaat caaatattat agatactcac tccatcacgt
961 gataatttca ctggtatgga aaagtggaaa attttataaa aaaaaatttg atgcctttgg
1021 catagctgaa acttcggccc aataggattg gagaatatgt tttcgcagcg ttcttacaat
1081 taaattgtgg tggaagttcg agacttgcgt aaactatttt taatttggag acggaattcc
1141 gtctcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
1201 aaagtggcac cgagtcggtg ctttttttct cgagtttttt tatcgagtta aaagctgaag
1261 tggtcaaagg aaccaattta attattgttc gtgaattagt tggtggtata tattttggtg
1321 aacgtcaaga acaagaagaa agtgaagaca aaaaaacagc ttgggataca gaaaagtata
1381 ctgttgacga agttactcgt atcactcgta tggcagcatt tatggctttg cagcacaatc
1441 caccattgcc aatatggtct ttggacaaag ctaatgtttt ggcatcgtcg agattgtgga
1501 gaaagaccgt tgataaagtg atttctgaag aatttccagc tttgtctgtg cagcatcaat
1561 tgattgattc ggctgcaatg attttgatcc aaaacccaac caaattgaac ggtatcatca
1621 ttacatctaa catgtttggt gatatcatct cagatgaggc atccgttatc ccaggatctt
1681 tgggtttatt gccatctgct tcattggctt cattgccaga taccaacact gcttttggtc
1741 tttatgagcc ttgccatggg tctgctccag atttaccagc caacaaagtg aatccaattg
1801 ctacaatctt gtctgccgct tccatgttga gattatcctt ggattgtgtt aaggaagctg
1861 aagcattgga agaagctgtc aaacaggttt tggatagcgg tatcagaaca gcagatttaa
1921 gaggtactag ctctactaca gaagtcggtg acgcaatagc cgaaacagtc actaaaattt
1981 taaaacaaaa tacataacgg tgtttaaacc ccagcgcctg gcgggcgctc acaattccac
2041 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
2101 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
2161 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
2221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
2281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
2341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
2401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
2461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
2521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
2581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
2641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
2701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
2761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
2821 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
2881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
2941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3001 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
3061 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
3121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttgatc agtgaggcac
3181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
3241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
3301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
3361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
3421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
3481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
3541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
3601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
3661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
3721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
3781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
3841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
3901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
3961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
4141 cacctgacgt
//
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