| 質粒名稱: | pAd-M3cherry |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 61041 |
| 存儲實驗室: | Qiao Zhou |
|
載體骨架基本信息 |
|
| 載體名稱: | pAd CMV/V5-DEST |
| 載體出品公司: | lifetechnologies |
| 空載體大小: | 36686 bp |
| 完整質粒大小: | 40200 bp |
| 載體類型: | Adenoviral |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱1: | Ngn3 / Neurog3 |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 642 bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | salI (not destroyed) |
| 3’克隆位點: | SpeI (not destroyed) |
| 5’測序引物 | ATGGCGCCTCATCCCT |
| 3’測序引物 | GTTC TCAGACTTCTTG |
| 插入基因名稱2: | pdx1 |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 852bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | BamHI (not destroyed) |
| 3’克隆位點: | BamHI (not destroyed) |
| 5’測序引物 | ATGAACAGTGAGGAG |
| 3’測序引物 | CAGGAACCCCGG |
| 插入基因名稱3: | Mafa |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 1077bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | ClaI (not destroyed) |
| 3’克隆位點: | ClaI (not destroyed) |
| 5’測序引物 | ATGGCCGCGGAGC |
| CACCCGACTTCTTTCTG | |
| 插入基因名稱4: | monomeric cherry |
| 物種來源: | Synthetic |
| 插入大小: | 711bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | NotI (not destroyed) |
| 3’克隆位點: | NotI (not destroyed) |
| 5’測序引物 | atggtgagcaagggcgagg |
| 3’測序引物 | ggacgagctgtacaagtaa |
|
文獻引用方法 |
|
| 材料和方法部分: |
pAd-M3cherry was a gift from Qiao Zhou (Addgene plasmid # 61041 ; //n2t.net/addgene:61041 ; RRID:Addgene_61041)
|
| 參考文獻部分: | Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Li W, Cavelti-Weder C, Zhang Y, Clement K, Donovan S, Gonzalez G, Zhu J, Stemann M, Xu K, Hashimoto T, Yamada T, Nakanishi M, Zhang Y, Zeng S, Gifford D, Meissner A, Weir G, Zhou Q. Nat Biotechnol. 2014 Dec;32(12):1223-30. doi: 10.1038/nbt.3082. Epub 2014 Nov 17. 10.1038/nbt.3082 PubMed 25402613 |
買家導航
pAd-M3cherry載體質粒基本信息
pAd-M3cherry質粒圖譜載體圖譜和pAd-M3cherry載體序列質粒序列多克隆位點信息
pAd-M3cherry質粒載體簡介
pAd-M3cherry質粒序列載體序列
LOCUS pAd-M3cherry 860 bp DNA linear UNA 08-JUL-2024
DEFINITION natural linear DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
REFERENCE 1 (bases 1 to 860)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 8, 2024 from SnapGene 6.0.2
//gt12365.cn
FEATURES Location/Qualifiers
source 1..860
/mol_type="genomic DNA"
/organism="unspecified"
ORIGIN
1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
121 aaaaaagcag gctggcgccg gaaccaattc agtcgacgcc gccaccatgg cgcctcatcc
181 cttggatgcg ctcaccatcc aagtgtcccc agagacacaa caaccttttc ccggagcctc
241 ggaccacgaa gtgctcagtt ccaattccac cccacctagc cccactctca tacctaggga
301 ctgctccgaa gcagaagtgg gtgactgccg agggacctcg aggaagctcc gcgcccgacg
361 cggagggcgc aacaggccca agagcgagtt ggcactcagc aaacagcgaa gaagccggcg
421 caagaaggcc aatgatcggg agcgcaatcg catgcacaac ctcaactcgg cgctggatgc
481 gctgcgcggt gtcctgccca ccttcccgga tgacgccaaa cttacaaaga tcgagaccct
541 gcgcttcgcc cacaactaca tctgggcact gactcagacg ctgcgcatag cggaccacag
601 cttctatggc ccggagcccc ctgtgccctg tggagagctg gggagccccg gaggtggctc
661 caacggggac tggggctcta tctactcccc agtctcccaa gcgggtaacc tgagccccac
721 ggcctcattg gaggaattcc ctggcctgca ggtgcccagc tccccatcct atctgctccc
781 gggagcactg gtgttctcag acttcttgac tagtgccacg aacttctctc tgttaaagca
841 agcaggagat gttgaagaaa
//
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