LOCUS       pCAGIG	6135 bp 	DNA	SYN
DEFINITION  pCAGIG
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="pCAGIG"
                     /mol_type="other DNA"
     misc_feature    84..371
                     /label="CAG_enhancer"
     CDS             255..734
                     /label="ORF frame 3"
     CDS             complement(802..1533)
                     /label="ORF frame 1"
     CDS             complement(843..1346)
                     /label="ORF frame 2"
     misc_feature    1778..2350
                     /label="IRES"
     CDS             2342..3073
                     /label="ORF frame 2"
     gene            2357..3070
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(2399..2420)
                     /label="EGFP_N_primer"
     misc_feature    2537..2566
                     /label="Y66 (EGFP)"
     misc_feature    3007..3028
                     /label="EGFP_C_primer"
     terminator      3127..3592
                     /label="rb_glob_PA_terminator"
     promoter        complement(3634..3652)
                     /label="M13_reverse_primer"
     misc_feature    complement(3651..3673)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3687..3716)
                     /label="lac_promoter"
     promoter        3822..4001
                     /label="SV40_promoter"
     rep_origin      3857..3934
                     /label="SV40_origin"
     misc_feature    3919..3938
                     /label="SV40pro_F_primer"
     terminator      4015..4146
                     /label="SV40_PA_terminator"
     misc_feature    4103..4122
                     /label="EBV_rev_primer"
     rep_origin      complement(4370..4989)
                     /label="pBR322_origin"
     gene            complement(5144..6004)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(5144..6004)
                     /label="ORF frame 3"
     promoter        complement(6046..6074)
                     /label="AmpR_promoter"
ORIGIN
    1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
   61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
  121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
  181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
  241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
  301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
  361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
  421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
  481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
  541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
  601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
  661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
  721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
  781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
  841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
  901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
  961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
 1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
 1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
 1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC
 1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
 1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT
 1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG
 1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC
 1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
 1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG
 1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
 1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
 1681 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG GATATCgcgg
 1741 ccgctacgta aattccgccc cccccccccc tctccctccc ccccccctaa cgttactggc
 1801 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
 1861 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
 1921 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
 1981 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
 2041 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
 2101 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
 2161 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
 2221 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
 2281 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
 2341 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
 2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
 2461 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
 2521 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
 2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
 2641 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
 2701 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
 2761 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
 2821 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
 2881 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
 2941 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
 3001 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
 3061 gctgtacaag taaagcggcc ggccgccagc acagtggtcg atcgacctgc aggcatgcaa
 3121 gcttcagCCA ATGCCCTGGC TCACAAATAC CACTGAGATC TTTTTCCCTC TGCCAAAAAT
 3181 TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG GAAATTTATT
 3241 TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC ATATGGGAGG
 3301 GCAAATCATT TAAAACATCA GAATGAGTAT TTGGTTTAGA GTTTGGCAAC ATATGCCATA
 3361 TGCTGGCTGC CATGAACAAA GGTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC
 3421 TGCTGTCCAT TCCTTATTCC ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT
 3481 TTGTTTTGTG TTATTTTTTT CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC
 3541 CAGATTTTTC CTCCTCTCCT GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGAAG
 3601 ATCCCTCGAC CTGCAGCCCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
 3661 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
 3721 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
 3781 AGTCGGGAAA CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC
 3841 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
 3901 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT
 3961 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT
 4021 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
 4081 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
 4141 TGGATCCGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
 4201 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
 4261 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
 4321 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
 4381 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
 4441 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
 4501 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
 4561 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
 4621 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
 4681 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
 4741 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
 4801 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
 4861 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
 4921 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
 4981 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
 5041 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
 5101 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
 5161 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
 5221 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
 5281 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
 5341 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
 5401 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
 5461 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
 5521 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
 5581 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
 5641 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
 5701 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
 5761 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
 5821 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
 5881 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
 5941 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
 6001 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
 6061 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC
 6121 GAAAAGTGCC ACCTG
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