| 質粒名稱: | pCAP03-acc(3)IV |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 69862 |
| 存儲實驗室: | Bradley Moore |
|
載體骨架基本信息 |
|
| 載體名稱: | pCAP01 |
| 載體出品公司: | -- |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | -- |
| 完整質粒大小: | -- |
| 載體修飾: | 在粟酒裂殖酵母 ADH1 基因 (pADH1) 的強啟動子下，將 URA3 基因導入 pCAP01，將其導入 pCAP01 的 SpeI 和 KpnI 限制性位點，作為計數器選擇標記，以產生 pCAP03。pADH1 可以容忍在 TATA 框和轉錄起始位點之間插入多達 130 bp 的片段。這種方法允許使用較短的捕獲臂，并在 5-氟乳清酸 (5-FOA) 存在下方便地選擇非同源末端連接。為了易于線性化 pCAP03，從 pIJ773 擴增 1369 bp 安普霉素抗性盒 (acc(3)IV) 并將其克隆到 pCAP03 中以獲得 pCAP03-acc(3)IV。 |
| 載體類型: | 細菌表達、酵母表達 |
| 篩選標記: | TRP1、URA3；安普霉素 |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | - |
| 標簽/融合蛋白: | -- |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | -- |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pCAP03-acc(3)IV was a gift from Bradley Moore (Addgene plasmid # 69862 ; //n2t.net/addgene:69862 ; RRID:Addgene_69862) |
| 參考文獻部分: | Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining. Tang X, Li J, Millan-Aguinaga N, Zhang JJ, O'Neill EC, Ugalde JA, Jensen PR, Mantovani SM, Moore BS. ACS Chem Biol. 2015 Oct 21. 10.1021/acschembio.5b00658 PubMed 26458099 |
買家導航
pCAP03-acc(3)IV載體質粒基本信息
pCAP03-acc(3)IV質粒圖譜載體圖譜和pCAP03-acc(3)IV載體序列質粒序列多克隆位點信息
IV質粒圖譜.png)
pCAP03-acc(3)IV質粒載體簡介
pCAP03-acc(3)IV質粒序列載體序列
LOCUS pCAP03-acc(3)IV 11875 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981).
ACCESSION .
VERSION .
KEYWORDS pCAP03-acc(3)IV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11875)
AUTHORS Tang X, Li J, Millan-Aguinaga N, Zhang JJ, O'Neill EC, Ugalde JA,
Jensen PR, Mantovani SM, Moore BS
TITLE Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways
by Target-directed Genome Mining.
JOURNAL ACS Chem Biol. 2015 Oct 21.
PUBMED 26458099
REFERENCE 2 (bases 1 to 11875)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..11875
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 733..766
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
oriT 773..882
/note="incP origin of transfer"
CDS 1198..1998
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/label=ApmR
/note="confers resistance to apramycin (Paget and Davies,
1996)"
/translation="MSLAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
protein_bind 2008..2054
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 2077..2880
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
misc_feature 2958..3461
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
primer_bind 3502..3520
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(3558..3580)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 3680..3699
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 3720..4000
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
CDS 4001..4675
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
primer_bind complement(4915..4936)
/label=F1ori-F
/note="F1 origin, forward primer"
rep_origin 5183..5771
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5672..5691
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5925..5942
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 6156..6290
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6180..6199)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 6234..6253
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(6715..6735)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 6865..6930
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS complement(7350..8144)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7461..7480)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 8071..8090
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
CDS complement(8928..9299)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 9332..9441
/note="incP origin of transfer"
CDS complement(9679..9690)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 9761..9860
/label=phage phi-C31 attP
/bound_moiety="phage phi-C31 integrase"
/note="attachment site of phage phi-C31"
CDS 9870..11687
/codon_start=1
/product="integrase from phage phi-C31"
/label=phage phi-C31 integrase
/translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
1 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
61 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
121 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
181 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
241 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
301 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
361 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
421 cgcttatcgc tgaccccctg ccgccctcct attggcttcc ggattatctt cttgtccata
481 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
541 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
601 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
661 aaaactcggt ttgacgcctc ccatggtata aatagtggct cgagattccg gggatccgtc
721 gacctgcagt tcgaagttcc tattctctag aaagtatagg aacttcgaag ttcccgccag
781 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
841 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
901 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
961 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaatgca
1021 gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac
1081 gctgaaaatg ccggcctttg aatgggttca tgtgcagctc cataagcaaa aggggatgat
1141 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg
1201 tcattagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt
1261 cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt
1321 agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctgcgctg
1381 ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat
1441 cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg
1501 ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag
1561 cagatcatct ctgatccatt gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt
1621 gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg
1681 ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt
1741 cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc
1801 tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt
1861 catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg
1921 ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc
1981 gctcgccagt cgattggctg agctcataag ttcctattcc gaagttccta ttctctagaa
2041 agtataggaa cttcgaagca gctccagcct acacatatgt cgaaagctac atataaggaa
2101 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
2161 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
2221 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
2281 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
2341 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
2401 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
2461 attgttagcg gtttgaagca ggcggcggaa gaagtaacaa aggaacctag aggccttttg
2521 atgttagcag aattgtcatg caagggctcc ctagctactg gagaatatac taagggtact
2581 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
2641 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
2701 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
2761 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
2821 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
2881 ggtacctgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
2941 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat
3001 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
3061 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
3121 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
3181 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
3241 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
3301 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa
3361 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca
3421 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtctaaga aaccattatt
3481 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
3541 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
3601 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
3661 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagatca
3721 cgacattact atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg
3781 tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc
3841 ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa
3901 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
3961 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
4021 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
4081 tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
4141 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
4201 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
4261 tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga
4321 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
4381 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
4441 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
4501 agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg
4561 tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa
4621 tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat
4681 tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat
4741 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg
4801 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
4861 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
4921 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
4981 tatcagggcg atggcccact acgtgaacct aactgtcaga ccaagtttac tcatatatac
5041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
5101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
5161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
5221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
5281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
5341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
5401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
5461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
5521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
5581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
5641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
5701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
5761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
5821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
5881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
5941 aggaagcgga agagcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
6001 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
6061 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
6121 ctcaacgaca ggagcacgat catgcgcacc cgtcagatcc agacatgata agatacattg
6181 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
6241 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
6301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
6361 aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa
6421 atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata
6481 gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt
6541 ataactgtta tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag
6601 cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa
6661 cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt
6721 tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga
6781 tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc actgctccca
6841 ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca gtagtttaac
6901 acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg taggtagttt
6961 gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatccgga ccaaagcggc
7021 catcgtgcct ccccactcct gcagttcggg ggcatggatg cgcggatagc cgctgctggt
7081 ttcctggatg ccgacggatt tgcactgccg gtagaactcc gcgaggtcgt ccagcctcag
7141 gcagcagctg aaccaactcg cgaggggatc gagcccgggg tgggcgaaga actccagcat
7201 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
7261 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
7321 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
7381 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
7441 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
7501 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
7561 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catccgcgcc
7621 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
7681 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
7741 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
7801 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
7861 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
7921 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca
7981 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
8041 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
8101 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
8161 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
8221 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
8281 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
8341 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
8401 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
8461 cagcccttgc gccctgagtg cttgcggcag cgtgagagct catgagcgga gaacgagatg
8521 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgtggagcgg atcggggatt
8581 gtctttcttc agctcgctga tgatatgctg acgctcaatg ccgtttggcc tccgactaac
8641 gaaaatcccg catttggacg gctgatccga ttggcacggc ggacggcgaa tggcggagca
8701 gacgctcgtc cgggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtatcgggc
8761 cctggccagc tagctagagt cgacctgcag gtccccgggg atcggtcttg ccttgctcgt
8821 cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
8881 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
8941 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
9001 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
9061 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
9121 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
9181 gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
9241 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
9301 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
9361 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
9421 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
9481 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
9541 ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgacctgc aggcatgcaa
9601 gctctagcga ttccagacgt cccgaaggcg tggcgcggct tccccgtgcc ggagcaatcg
9661 ccctgggtgg gttacacgac gcccctctat ggcccgtact gacggacaca ccgaagcccc
9721 ggcggcaacc ctcagcggat gccccggggc ttcacgtttt cccaggtcag aagcggtttt
9781 cgggagtagt gccccaactg gggtaacctt tgagttctct cagttggggg cgtagggtcg
9841 ccgacatgac acaaggggtt gtgaccgggg tggacacgta cgcgggtgct tacgaccgtc
9901 agtcgcgcga gcgcgaaaat tcgagcgcag caagcccagc gacacagcgt agcgccaacg
9961 aagacaaggc ggccgacctt cagcgcgaag tcgagcgcga cgggggccgg ttcaggttcg
10021 tcgggcattt cagcgaagcg ccgggcacgt cggcgttcgg gacggcggag cgcccggagt
10081 tcgaacgcat cctgaacgaa tgccgcgccg ggcggctcaa catgatcatt gtctatgacg
10141 tgtcgcgctt ctcgcgcctg aaggtcatgg acgcgattcc gattgtctcg gaattgctcg
10201 ccctgggcgt gacgattgtt tccactcagg aaggcgtctt ccggcaggga aacgtcatgg
10261 acctgattca cctgattatg cggctcgacg cgtcgcacaa agaatcttcg ctgaagtcgg
10321 cgaagattct cgacacgaag aaccttcagc gcgaattggg cgggtacgtc ggcgggaagg
10381 cgccttacgg cttcgagctt gtttcggaga cgaaggagat cacgcgcaac ggccgaatgg
10441 tcaatgtcgt catcaacaag cttgcgcact cgaccactcc ccttaccgga cccttcgagt
10501 tcgagcccga cgtaatccgg tggtggtggc gtgagatcaa gacgcacaaa caccttccct
10561 tcaagccggg cagtcaagcc gccattcacc cgggcagcat cacggggctt tgtaagcgca
10621 tggacgctga cgccgtgccg acccggggcg agacgattgg gaagaagacc gcttcaagcg
10681 cctgggaccc ggcaaccgtt atgcgaatcc ttcgggcccc gcgtattgcg ggcttcgccg
10741 ctgaggtgat ctacaagaag aagccggacg gcacgccgac cacgaagatt gagggttacc
10801 gcattcagcg cgacccgatc acgctccggc cggtcgagct tgattgcgga ccgatcatcg
10861 agcccgctga gtggtatgag cttcaggcgt ggttggacgg cagggggcgc ggcaaggggc
10921 tttcccgggg gcaagccatt ctgtccgcca tggacaagct gtactgcgag tgtggcgccg
10981 tcatgacttc gaagcgcggg gaagaatcga tcaaggactc ttaccgctgc cgtcgccgga
11041 aggtggtcga cccgtccgca cctgggcagc acgaaggcac gtgcaacgtc agcatggcgg
11101 cactcgacaa gttcgttgcg gaacgcatct tcaacaagat caggcacgcc gaaggcgacg
11161 aagagacgtt ggcgcttctg tgggaagccg cccgacgctt cggcaagctc actgaggcgc
11221 ctgagaagag cggcgaacgg gcgaaccttg ttgcggagcg cgccgacgcc ctgaacgccc
11281 ttgaagagct gtacgaagac cgcgcggcag gcgcgtacga cggacccgtt ggcaggaagc
11341 acttccggaa gcaacaggca gcgctgacgc tccggcagca aggggcggaa gagcggcttg
11401 ccgaacttga agccgccgaa gccccgaagc ttccccttga ccaatggttc cccgaagacg
11461 ccgacgctga cccgaccggc cctaagtcgt ggtgggggcg cgcgtcagta gacgacaagc
11521 gcgtgttcgt cgggctcttc gtagacaaga tcgttgtcac gaagtcgact acgggcaggg
11581 ggcagggaac gcccatcgag aagcgcgctt cgatcacgtg ggcgaagccg ccgaccgacg
11641 acgacgaaga cgacgcccag gacggcacgg aagacgtagc ggcgtagcga gacacccggg
11701 aagcctgatc tacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
11761 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
11821 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcga ctagt
//