LOCUS pCMV-HsPeg10rc3 6424 bp ds-DNA circular SYN 14-SEP-2021
DEFINITION Expresses recoded human Peg10 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-HsPeg10rc3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6424)
AUTHORS Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
RK, Koonin EV, Zhang F
TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and
can be pseudotyped for mRNA delivery.
JOURNAL Science. 2021 Aug 20;373(6557):882-889. doi:
10.1126/science.abg6155.
PUBMED 34413232
REFERENCE 2 (bases 1 to 6424)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 14, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..6424
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 201..225
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 338..813
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
CDS 3115..3141
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
polyA_signal 3226..3620
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
primer_bind complement(3959..3976)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4130..4718)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4210..4229)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4889..5749)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5512..5531
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5750..5854)
/gene="bla"
/label=AmpR promoter
primer_bind complement(5924..5943)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 6045..6424
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgc tgggtcccga
901 ctgcccacct cctcctcctc cccctccccc caacaacaac aacaacaaca actccaagca
961 caccggccat aagagtgcgt gtgtccccaa catgaccgaa cgaagaaggg acgagctctc
1021 tgaagagatc aacaacttaa gagagaaggt catgaagcag tcggaggaga acaacaacct
1081 gcagagccag gtgcagaagc tcacagagga gaacaccacc cttcgagagc aagtggaacc
1141 cacccctgag gatgaggatg atgacatcga gctccgcggt gctgcagcag ctgctgcccc
1201 accccctcca atagaggaag agtgcccaga agacctccca gagaagttcg atggcaaccc
1261 agacatgctg gctcctttca tggcccagtg ccagatcttc atggaaaaga gcaccaggga
1321 tttctcagtt gatcgtgtcc gtgtctgctt cgtgacaagc atgatgaccg gccgtgctgc
1381 ccgttgggcc tcagcaaagc tggagcgctc ccactacctg atgcacaact acccagcttt
1441 catgatggaa atgaagcatg tctttgaaga ccctcagagg cgagaggttg ccaaacgcaa
1501 gatcagacgc ctgcgccaag gcatggggtc tgtcatcgac tactccaatg ctttccagat
1561 gattgcccag gacctggatt ggaacgagcc tgcgctgatt gaccagtacc acgagggcct
1621 cagcgaccac attcaggagg agctctccca cctcgaggtc gccaagtcgc tgtctgctct
1681 gattgggcag tgcattcaca ttgagagaag gctggccagg gctgctgcag ctcgcaagcc
1741 acgctcgcca ccccgggcgc tggtgttgcc tcacattgca agccaccacc aggtagatcc
1801 aaccgagccg gtgggaggtg cccgcatgcg cctgacgcag gaagaaaaag aaagacgcag
1861 aaagctgaac ctgtgcctct actgtggaac aggaggtcac tacgctgaca attgtcctgc
1921 caaggcctca aagtcttcgc cggcgggaaa ctccccggcc ccgctgtaga gggaccttca
1981 gcgaccgggc cagaaataat aaggtcccca caagatgatg cctcatctcc acacttgcaa
2041 gtgatgcttc agatacatct gccaggcagg cacacactct tcgtacgagc tatgattgat
2101 tcaggggcat ctggtaactt catagatcac gagtatgtag ctcagaacgg aattcctttg
2161 agaataaagg actggcccat cttggttgaa gctatcgatg ggaggcctat agcttccggt
2221 cccgtggtgc atgaaacaca tgatcttatt gtcgatcttg gggatcaccg cgaagtcctc
2281 tcatttgacg taacccagtc ccctttcttc cccgtggtcc tcggagtgcg gtggctgagt
2341 actcacgatc ctaatattac atggagcacc cggtcaatcg tatttgatag tgagtactgt
2401 aggtaccact gtcgaatgta tagtcctatt ccccctagtt tgcctccccc agctcctcaa
2461 ccacccctgt actatcccgt ggacggatat agggtgtacc agcccgttcg atattactac
2521 gtacaaaatg tttatacacc tgttgatgag cacgtctacc cagaccatag actcgtcgac
2581 cctcacatcg aaatgattcc aggagcccat agtatacctt cagggcacgt atactctctg
2641 tccgaacctg aaatggcagc tcttcgagat tttgtggcaa gaaatgtaaa agatgggcta
2701 attactccaa cgattgcacc taatggagcc caagttctcc aggtgaagag ggggtggaaa
2761 ctgcaagttt cttatgattg ccgagctcca aacaatttta ctatccagaa tcagtatcct
2821 cgcctatcta ttccaaattt agaagaccaa gcacacctgg caacgtacac tgaattcgta
2881 cctcaaatac ctggatacca aacatacccc acatatgccg cgtacccgac ctacccagta
2941 ggattcgcct ggtacccagt gggacgagac ggacaaggaa gatcactata tgtacctgtg
3001 atgatcactt ggaatccaca ctggtaccgc cagcctccgg taccacagta cccgccgcca
3061 cagccgccgc ctccaccacc accaccgccg ccgcctccat cttacagtac cctgtatccg
3121 tatgatgttc cggattatgc atgagaattc accccaccag tgcaggctgc ctatcagaaa
3181 gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg ctttcttgct
3241 gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat
3301 tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt tcattgcaat
3361 gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag gtcagtgcat
3421 ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact atatcttaaa
3481 ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag cccctgatgc
3541 ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg gaggttaaag
3601 ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga atgtcttttc
3661 actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc tcttgcttag
3721 agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg gtttctcctc
3781 gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt gaagaaggaa
3841 aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc cccactcaca
3901 gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga tctgcttcct
3961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
4861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
5521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5821 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
5881 acgtggatcc cctgaggggg cccccatggg ctagaggatc cggcctcggc ctctgcataa
5941 ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc catatcataa
6001 tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt gattattgac
6061 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
6121 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
6181 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
6241 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
6301 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
6361 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
6421 catg
//
