LOCUS        pCMV-PE7       10473 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of SpCas9 PE7 prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y,
            Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, 
            Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B
  TITLE     Improving prime editing with an endogenous small RNA-binding 
            protein.
  JOURNAL   Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6.
  PUBMED    38570691
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Apr 3. doi: 10.1038/s41586-024-07259-6."
COMMENT     Sequence Label: pCMV-PE7
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1215..1234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1940..1960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2031..2050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2099..2119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2120..6220
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6278..6298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9077..9097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9107..9133
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             9142..9159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9182..9199)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9188..9412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9483..9499)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9483..9499)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9496..9518)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9507..9523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9531..9537,9538..9555,9556..9561))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9576..9597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9714..9731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9885..10473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9965..9984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     2101 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2161 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2221 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2281 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2341 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2401 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2461 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2521 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2581 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2641 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2701 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2761 actgagcaag agcagaaagc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2821 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     2881 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     2941 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3001 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3061 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3121 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3181 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3241 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaagag
     3301 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3361 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3421 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3481 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     3541 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3601 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3661 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3721 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3781 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3841 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     3901 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     3961 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4021 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4081 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4141 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4201 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4261 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4321 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4381 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4441 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4501 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4561 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4621 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4681 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4741 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4801 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4861 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     4921 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     4981 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5041 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5101 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5161 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5221 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5281 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5341 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5401 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5461 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     5521 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5581 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5641 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5701 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5761 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5821 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     5881 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     5941 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6001 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6061 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6121 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6181 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg
     6241 cagcaagcgg accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag
     6301 cggaggctct agcggcggaa gcaccctgaa cattgaagac gagtatagac tgcatgaaac
     6361 aagcaaggaa cccgacgtgt ccctgggctc cacctggctg tccgactttc cccaggcctg
     6421 ggccgagaca ggaggaatgg gcctggccgt gcggcaggca cccctgatca tccctctgaa
     6481 ggccacctct acacccgtga gcatcaagca gtaccctatg tctcaggagg ccagactggg
     6541 catcaagcct cacatccaga ggctgctgga ccagggcatc ctggtgccat gccagagccc
     6601 ctggaacaca ccactgctgc ccgtgaagaa gccaggcacc aatgactata gacccgtgca
     6661 ggatctgaga gaggtgaaca agagggtgga ggatatccac cccaccgtgc ccaaccctta
     6721 caatctgctg tccggcctgc ccccttctca ccagtggtat acagtgctgg acctgaagga
     6781 tgccttcttt tgtctgagac tgcaccctac cagccagcca ctgttcgcct ttgagtggag
     6841 ggaccctgag atgggcatct ctggccagct gacctggaca cgcctgcctc agggcttcaa
     6901 gaatagccca acactgttta acgaggccct gcaccgcgac ctggcagatt tccggatcca
     6961 gcacccagat ctgatcctgc tgcagtacgt ggacgatctg ctgctggccg ccaccagcga
     7021 gctggattgc cagcagggaa cacgcgccct gctgcagacc ctgggaaacc tgggatatag
     7081 ggcatccgcc aagaaggccc agatctgtca gaagcaggtg aagtacctgg gctatctgct
     7141 gaaggagggc cagagatggc tgacagaggc caggaaggag acagtgatgg gccagccaac
     7201 acccaagacc ccaagacagc tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt
     7261 catcccagga ttcgcagaga tggcagcacc tctgtaccca ctgaccaagc cgggcaccct
     7321 gtttaattgg ggccctgacc agcagaaggc ctatcaggag atcaagcagg ccctgctgac
     7381 agcaccagcc ctgggcctgc cagacctgac caagcctttc gagctgtttg tggatgagaa
     7441 gcagggctac gccaagggcg tgctgaccca gaagctggga ccatggagac ggcccgtggc
     7501 ctatctgtcc aagaagctgg acccagtggc agcaggatgg ccaccatgcc tgaggatggt
     7561 ggcagcaatc gccgtgctga caaaggatgc cggcaagctg accatgggac agccactggt
     7621 catcctggca ccacacgcag tggaggccct ggtgaagcag cctccagatc gctggctgtc
     7681 taacgcccgg atgacacact accaggccct gctgctggac accgatcgcg tgcagtttgg
     7741 ccctgtggtg gccctgaatc cagccaccct gctgcctctg ccagaggagg gcctgcagca
     7801 caactgtctg gacatcctgg cagaggcaca cggaacaagg ccagacctga ccgatcagcc
     7861 cctgcctgac gccgatcaca catggtatac cgatggaagc tccctgctgc aggagggcca
     7921 gaggaaggca ggagcagcag tgaccacaga gacagaagtg atctgggcca aggccctgcc
     7981 agcaggcaca tccgcccagc gggccgagct gatcgccctg acccaggccc tgaagatggc
     8041 cgagggcaag aagctgaacg tgtacacaga ctccagatat gccttcgcca ccgcacacat
     8101 ccacggagag atctacaggc gccggggctg gctgacctct gagggcaagg agatcaagaa
     8161 caaggatgag atcctggccc tgctgaaggc cctgtttctg cccaagcggc tgagcatcat
     8221 ccactgtcct ggacaccaga agggacactc cgccgaggca aggggcaatc ggatggccga
     8281 ccaggccgcc agaaaggctg ctattactga aactcccgac acttccactc tgctgattga
     8341 aaactcctcc ccttccggag gatctagcgg aggctcctct ggctctgaga cacctggcac
     8401 aagcgagagc gcaacacctg aaagcagcgg gggcagcagc ggggggtcaa tggctgaaaa
     8461 tggtgataat gaaaagatgg ctgccctgga ggccaaaatc tgtcatcaaa ttgagtatta
     8521 ttttggcgac ttcaatttgc cacgggacaa gtttctaaag gaacagataa aactggatga
     8581 aggctgggta cctttggaga taatgataaa attcaacagg ttgaaccgtc taacaacaga
     8641 ctttaatgta attgtggaag cattgagcaa atccaaggca gaactcatgg aaatcagtga
     8701 agataaaact aaaatcagaa ggtctccaag caaaccccta cctgaagtga ctgatgagta
     8761 taaaaatgat gtaaaaaaca gatctgttta tattaaaggc ttcccaactg atgcaactct
     8821 tgatgacata aaagaatggt tagaagataa aggtcaagta ctaaatattc agatgagaag
     8881 aacattgcat aaagcattta agggatcaat ttttgttgtg tttgatagca ttgaatctgc
     8941 taagaaattt gtagagaccc ctggccagaa gtacaaagaa acagacctgc taatactttt
     9001 caaggacgat tactttgcca aaaaaaatga atctggcggc tcaaaaagaa ccgccgacgg
     9061 cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg ccgctaagag
     9121 agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     9181 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa aattgcatcg
     9301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9421 gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca
     9481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9541 gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt
     9601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10441 cagcagatta cgcgcagaaa aaaaggatct caa
//