| 質粒名稱: | pCMV5-FLAG-MKK6 |
|---|---|
| 出品公司: | addgene |
| 目錄編號: | 21582 |
| 存儲實驗室: | John Kyriakis |
|
載體骨架基本信息 |
|
| 載體名稱: | pCMV5 |
| 載體出品公司: | Dr. David Russell, UTSW |
| 空載體大小: | 4700 bp |
| 完整質粒大小: | 4700 bp |
| 載體類型: | 哺乳細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | MKK6 / MEK6 |
| 物種來源: | H. sapiens (human) |
| 標簽/融合蛋白: | FLAG |
| 克隆方法: | 限制性內切酶 |
| 5’測序引物 | CMV-F |
|
文獻引用方法 |
|
| 材料和方法部分: | pCMV5-FLAG-MKK6 was a gift from John Kyriakis (Addgene plasmid # 21582 ; //n2t.net/addgene:21582 ; RRID:Addgene_21582) |
| 參考文獻部分: | Tumor necrosis factor signaling to stress-activated protein kinase (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 1 and SAPK while receptor interacting protein associates with a mitogen-activated protein kinase kinase kinase upstream of MKK6 and p38. Yuasa T, Ohno S, Kehrl JH, Kyriakis JM. J Biol Chem. 1998 Aug 28. 273(35):22681-92. 10.1074/jbc.273.35.22681 PubMed 9712898 |
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pCMV5-FLAG-MKK6載體質粒基本信息
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