| 質粒名稱: | pCalN-ddGFP-A |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 40290 |
| 存儲實驗室: | Robert Campbell |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3.1 |
| 載體出品公司: | Invitrogen |
| 載體抗性: | 氨芐青霉素 |
| 載體類型: | 哺乳細胞表達 |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | Calnexin,CANX |
| 物種來源: | H. sapiens (human) |
| 啟動子 | CMV |
| 標簽/融合蛋白: | ddGFP-A（插入件上的C端子） |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | XhoI(未破壞) |
| 3' 克隆位點: | HindIII(未破壞) |
| 5' 測序引物 | TAATACGACTCACTATAGGG |
| 3' 測序引物 | TAGAAGGCACAGTCGAGG |
|
文獻引用方法 |
|
| 材料和方法部分: | pCalN-ddGFP-A was a gift from Robert Campbell (Addgene plasmid # 40290 ; //n2t.net/addgene:40290 ; RRID:Addgene_40290) |
| 參考文獻部分: | Dimerization-dependent green and yellow fluorescent proteins. Alford SC, Ding Y, Simmen T, Campbell RE. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. 10.1021/sb300050j PubMed 23656278 |
買家導航
pCalN-ddGFP-A載體質粒基本信息
pCalN-ddGFP-A質粒圖譜載體圖譜和pCalN-ddGFP-A載體序列質粒序列多克隆位點信息
pCalN-ddGFP-A質粒載體簡介
pCalN-ddGFP-A質粒序列載體序列
LOCUS pCalN-ddGFP-A 7885 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCalN-ddGFP-A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7885)
AUTHORS Alford SC, Ding Y, Simmen T, Campbell RE
TITLE Dimerization-dependent green and yellow fluorescent proteins.
JOURNAL ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j.
Epub 2012 Aug 14.
PUBMED 23656278
REFERENCE 2 (bases 1 to 7885)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..7885
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 906..915
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2875..2893)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3143..3162
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 3337..3360
/label=DsRed1-C
/note="DsRed1, forward primer"
primer_bind complement(3476..3493)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3482..3706
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3752..4180
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3839..3858)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4049..4070
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4189..4209)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4194..4523
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4374..4509
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4436..4455
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4590..5384
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4644..4663)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5254..5273
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5558..5679
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5595..5614)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5649..5668
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5728..5744)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5728..5744)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5741..5763)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5752..5768
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5776..5806)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5821..5842
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5959..5976)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6130..6718)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6210..6229)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6889..7749)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7512..7531
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7750..7854)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtgccgccac catggcctcg agaatggaag ggaagtggtt gctgtgtatg ttactggtgc
961 ttggaactgc tattgttgag gctcatgatg gacatgatga tgatgtgatt gatattgagg
1021 atgaccttga cgatgtcatt gaagaggtag aagactcaaa accagatacc actgctcctc
1081 cttcatctcc caaggttact tacaaagctc cagttccaac aggggaagta tattttgctg
1141 attcttttga cagaggaact ctgtcagggt ggattttatc caaagccaag aaagacgata
1201 ccgatgatga aattgccaaa tatgatggaa agtgggaggt agaggaaatg aaggagtcaa
1261 agcttccagg tgataaagga cttgtgttga tgtctcgggc caagcatcat gccatctctg
1321 ctaaactgaa caagcccttc ctgtttgaca ccaagcctct cattgttcag tatgaggtta
1381 atttccaaaa tggaatagaa tgtggtggtg cctatgtgaa actgctttct aaaacaccag
1441 aactcaacct ggatcagttc catgacaaga ccccttatac gattatgttt ggtccagata
1501 aatgtggaga ggactataaa ctgcacttca tcttccgaca caaaaacccc aaaacgggta
1561 tctatgaaga aaaacatgct aagaggccag atgcagatct gaagacctat tttactgata
1621 agaaaacaca tctttacaca ctaatcttga atccagataa tagttttgaa atactggttg
1681 accaatctgt ggtgaatagt ggaaatctgc tcaatgacat gactcctcct gtaaatcctt
1741 cacgtgaaat tgaggaccca gaagaccgga agcccgagga ttgggatgaa agaccaaaaa
1801 tcccagatcc agaagctgtc aagccagatg actgggatga agatgcccct gctaagattc
1861 cagatgaaga ggccacaaaa cccgaaggct ggttagatga tgagcctgag tacgtacctg
1921 atccagacgc agagaaacct gaggattggg atgaagacat ggatggagaa tgggaggctc
1981 ctcagattgc caaccctaga tgtgagtcag ctcctggatg tggtgtctgg cagcgacctg
2041 tgattgacaa ccccaattat aaaggcaaat ggaagcctcc tatgattgac aatcccagtt
2101 accagggaat ctggaaaccc aggaaaatac caaatccaga tttctttgaa gatctggaac
2161 ctttcagaat gactcctttt agtgctattg gtttggagct gtggtccatg acctctgaca
2221 ttttttttga caactttatc atttgtgctg atcgaagaat agttgatgat tgggccaatg
2281 atggatgggg cctgaagaaa gctgctgatg gggctgctga gccaggcgtt gtggggcaga
2341 tgaacgaggc agctgaagag cgcccgtggc tgtgggtagt ctatattcta actgtagccc
2401 ttcctgtgtt cctggttatc ctcttctgct gttctggaaa gaaacagacc agtggtatgg
2461 agtataagaa aactgatgca cctcaaccgg atgtgaagga agaggaagaa gagaaggaag
2521 aggaaaagga caagggagat gaggaggagg aaggagaaga gaaacttgaa gagaaacaga
2581 aaagtgatgc tgaagaagat ggtggcactg tcagtcaaga ggaggaagac agaaaaccta
2641 aagcagagga ggatgaaatt ttgaacagat caccaagaaa cagaaagcca cgaagagagg
2701 ggggtgcgtc aggcggcagc ggatctggac cggtcggtac catggtgagc aagagcgagg
2761 aggtcatcaa agagttcatg cgcttcaagg tgcgcttgga gggctccatg aacggccacg
2821 agttcgagat cgagggcgag ggcgagggcc gcccttacga gggcacccag accgccaagc
2881 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc cccctgatca
2941 tgtacggctc caagatgtac gtgaagcacc ccgccgacgt ccccgattac atgaagctgt
3001 ccttccccga gggcttcaag tgggagcgcg tgatgcactt cgaggacggc ggtctggtga
3061 ccgcgacaca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
3121 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccttgggc tgggattatg
3181 ccaccgagcg cctgtacccc gaagaaggcg tgctgaaggg cgagctcctg gggcgcctga
3241 agctgaagga cggcggcctc aacctggtgg agtccaagac catctacatg gccaagaagc
3301 ccgtgcaact gcccggctac tacttcgtgg acaccaagct ggacatcacc tcccacaacg
3361 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctggggcatg
3421 gtacaggtag cacaggcagc ggtagctcct aaaagcttgg taaaccgctg atcagcctcg
3481 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
3541 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
3601 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
3661 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
3721 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
3781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
3841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
3901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
3961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
4141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
4201 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
4261 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4321 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
4381 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
4441 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
4501 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
4561 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
4621 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
4681 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
4741 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
4801 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
4861 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
4921 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
4981 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
5041 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
5101 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
5161 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
5221 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
5281 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
5341 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
5401 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
5461 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
5521 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
5701 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
5761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
5821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
5881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
5941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
6001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
6061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
6121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
6181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
6241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
6301 ttcgggaagc gtggcgcttt ctcatagctc acgctatagg tatctcagtt cggtgtaggt
6361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
6421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
6481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
6541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
6601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
6661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
6721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
6781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
6841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
6901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
6961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
7021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
7081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
7141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
7201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
7261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
7321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
7381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
7441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
7501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
7561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
7621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
7681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
7741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
7801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
7861 tccccgaaaa gtgccacctg acgtc
//
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