| 質粒名稱: | pExchange_tdk_BT1311p-tetR-1 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 90325 |
| 存儲實驗室: | Vladislav Verkhusha |
|
載體骨架基本信息 |
|
| 載體名稱: | pExchange-tdk |
| 載體出品公司: | Clontech |
| 空載體大小: | 4038 bp |
| 完整質粒大小: | 4989 bp |
| 載體類型: | 細菌表達 |
| 篩選標記: | Erythromycin |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | Pir1 |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | TetR |
| 克隆方法: | Gibson Cloning |
| 5’測序引物 | GGAGAGACAGCCGAATACGA |
| 3’測序引物 |
AACACTTAACGGCTGACATGG
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | pExchange_tdk_BT1311p-tetR-1 was a gift from Andrew Goodman (Addgene plasmid # 90325 ; //n2t.net/addgene:90325 ; RRID:Addgene_90325) |
| 參考文獻部分: | Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut. Lim B, Zimmermann M, Barry NA, Goodman AL. Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. 10.1016/j.cell.2017.03.045 PubMed 28431252 |
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pExchange_tdk_BT1311p-tetR-1載體質粒基本信息
pExchange_tdk_BT1311p-tetR-1質粒圖譜載體圖譜和pExchange_tdk_BT1311p-tetR-1載體序列質粒序列多克隆位點信息
pExchange_tdk_BT1311p-tetR-1質粒載體簡介
pExchange_tdk_BT1311p-tetR-1質粒序列載體序列
LOCUS pExchange_tdk_BT1311p-tetR-1 7210 bp DNA circular SYN 25-AUG-2024
DEFINITION Suicide vector for integration of a constitutively expressed TetR by
promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and
4861702 .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7210)
AUTHORS Lim B, Zimmermann M, Barry NA, Goodman AL
TITLE Engineered Regulatory Systems Modulate Gene Expression of Human
Commensals in the Gut.
JOURNAL Cell. 2017 Apr 20;169(3):547-558.e15. doi:
10.1016/j.cell.2017.03.045.
PUBMED 28431252
REFERENCE 2 (bases 1 to 7210)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cell.2017.03"; journalName: "Cell"; date: "2017-04-20-
20"; volume: "169"; issue: "3"; pages: "547-558.e15"
FEATURES Location/Qualifiers
source 1..7210
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(30..890)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 653..672
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS 4713..5336
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(4786..4805)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
rep_origin 6441..6829
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
oriT 7000..7109
/note="incP origin of transfer"
ORIGIN
1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
2641 aatcaagaaa gcacaaaaat cacatggtat tattttcaga agaccacata caagagacca
2701 gaagaagagg tagaatagag gtaatctgcg gttcaatgtt ctccggaaag acggaggaat
2761 tgattcgtag gatgaagaga gccaagttcg cacgccagcg tgtagagata ttcaaaccgg
2821 ccatcgacac ccgttattcg gaagaagacg tggtatcaca cgacagtcat tccatcgcgt
2881 ccacccctat cgattcatcg gctagtatcc tgttgttcac ttctgaaata gatgtagtcg
2941 gcatcgacga agcacagttc tttgatgacg gactgattga cgtatgcagg cagcttgcca
3001 ataacggcat ccgtgtcatc atcgccggac tggatatgga ctttaaaggg aatcccttcg
3061 gaccgatgcc tcaattatgt gccattgccg atgaagtttc caaagttcat gcgatctgcg
3121 tgaagtgcgg tgatctggca tctttctctc atcgtaccgt caagaatgac aagcaggtac
3181 ttctaggaga gacagccgaa tacgagccgc tttgcaggga gtgctacctc cgtgcacggg
3241 gagaggacgg acagaagata taaactctat ttaaaacata tatggaaaga aagaagataa
3301 cattcgagtc gacggtatcg ataagcttga tatcgaattc ctgcaatgcg aatgtagagg
3361 ggaaaataga tgggaataat attagtttta ttattccaga tggattggag aacttgacgt
3421 ttgcgcctac tattgttatt tctgataaag caacaataac tcctgcttct ggagtagaac
3481 aagatttttc atctcctgta atatacacag tgacttccga agatggaatt gtaactacta
3541 aatatacagt ttctgtatca ggtcaaataa gagattttgg attcgatgaa tgggagacta
3601 tcaaatcatc tacatcgggt tctacagaac aatatgatgt tcctaaagga ctttatggta
3661 ctagtaatcc gggaattatg acaattaacg agatgtttgg ttcaaatctt ccagagctgt
3721 tctcatatac agtttctcct gtagaaggac aaagtggtaa agcagctaaa ttgcaaacag
3781 tttatactta catgttttta aatggtatgg attttaatgc attattagga gggttggtac
3841 cttatgtaac agcaggctca ttatttacag gtatctttaa aactgatatg aataatactt
3901 tgaatagtac aaagtttggt gttccttttg ttggggaacc tgctactttt actggctatt
3961 ataaatatac tccagggaca acatattata ataataaaaa tgaagtcgta gctgataaaa
4021 cagatgaatg ttcaatatat gcagttttgt atgaagaact tttggatgaa gagaataaca
4081 acattccgtt gaatggagac tacaatgatc ctgatctata tattggaagc tctaaacgta
4141 ttgtaatgaa ggccgttttg gaagatggaa cagcgaagag tgattggact aagttctcta
4201 tcccatttaa attattggaa gggaaaagtt atgatgcaac taaaaaatat tatttagcta
4261 ttgtgtgttc ctcaagtgct gaaggagact actttatggg cgctcctggt agtaccttat
4321 ttattgatag ttttacggta acacctcaat agtgtgttga gtataagaaa tatataacag
4381 aaaaagccgt taattccaat tgtatacatt aatgatctgg aagaagcaat gaaagctgct
4441 gttaagtctc cgaatcaggt attgttcctg acaggtgtat tcccatccgg taaacgcgga
4501 tactttgcag ttgatctgac tcaggaataa attataaatt aaggtaagaa gattgtagga
4561 taagctaatg aaatagaaaa aggatgccgt cacacaactt gtcggcattc ttttttgttt
4621 tattagttga aaatatagtg aaaaagttgc ctaaatatgt atgttaacaa attatttgtc
4681 gtaactttgc actccaaatc tgtttttaaa gaatgtctag attagataaa agtaaagtga
4741 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
4801 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
4861 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
4921 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
4981 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
5041 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
5101 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
5161 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
5221 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
5281 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaatgca
5341 attaaaatta acggcttttt tagttgtttt tatttaaata aattgtagtg ttttctctag
5401 aacgcatatc cgaatcctat attcaaataa ataggataca tagcgaatga gatggtatca
5461 aaatcctttt tgaagatatc gttcaagccc catgttaaat cagcatacac atttaaatgt
5521 ttgaatgctc tccactcacc gcctagttgc aatccccatt ggaatttacg cagattgtca
5581 gagaaatcat aagtagcaat gctttcacca gtaaaatcta ctcgcgaacc tgtctgatcc
5641 ggagtacgca agtgaccttc atatacatgt ccggagaagt ttctttcaat aagataagat
5701 acatacggac ctgctgagac tttccaacgt ttgcttactt tatagttggc aactactggg
5761 atagtcagat aagagttttt gactttagtc tttacaccac cggtccataa gccttgtaac
5821 tctccaccgt tggtgttgat aatcttcatc ccatagttct ttactgtagc ttctgtggtc
5881 atattcttgt tttcaagtcg tacccctaca gtcattcccc atttcgtgtc aaaccattta
5941 gttgcgtttc cttcaattga aatggcgagg ccaccagggg catagctatc tattttgcga
6001 atttcttcag gtagaggcaa tggagaagtt cctccaatac tgaaacctgc cttgattcca
6061 tattcccatc cgtgtaagta cgaccagatg ataccttgat ttctttcttc ttgcgcctgc
6121 gctgaaagtg tacttactaa tagtagtaca gctataatat tgagtaattt atatcttttc
6181 attttcttgt ttttttagtc ttcgcaaata agttctactt catcgacata cagtgtactg
6241 cctactgctc ccttaaaata agcaccgtct acactggacg aaagcactat acctaatttg
6301 tattttccat tctttaattt ggtatcatca attactcgtc cttttaccgg ttcgaaagga
6361 atttcaaaat cagtccattg atctgattca acaacatctt ccggtttaat tcgcgccacc
6421 gcggtggagg ggaattccca tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt
6481 tgagaggctc taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta
6541 aaccttaaaa gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt
6601 agaggctatt taagttgctg atttatatta attttattgt tcaaacatga gagcttagta
6661 cgtgaaacat gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt
6721 ttagttcgtt aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat
6781 gagagcttag tacgtactat caacaggttg aactgctgat cttcagatcc tctacgccgg
6841 acgcatcgtg gccggatcaa ttccgttttc cgctgcataa ccctgcttcg gggtcattat
6901 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
6961 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
7021 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
7081 cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg agggcaagcg
7141 gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcacgg aattgatccc
7201 cctcgaattg
//
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