| 質粒名稱: | pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 19291 |
| 存儲實驗室: | Eric Fearon |
|
載體骨架基本信息 |
|
| 載體名稱: | pGL2Basic |
| 載體出品公司: | Promega |
| 載體抗性: | 氨芐霉素 |
| 空載體大小: | 5568 bp |
| 載體類型: | 哺乳細胞表達,熒光素酶 |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | E-cadherin |
| 物種來源: | H. sapiens (人) |
| 插入大小: | 233 bp |
| 基因突變: | E-cad promoter sequences from -108 to +125 Ebox elements were mutated from 5'-CANNTG-3' to 5'-AANNTA-3' (sense strand) |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | SacI(未破壞) |
| 3' 克隆位點: | HindIII (未破壞) |
| 5' 測序引物 | 未知 |
| 3' 測序引物 | luc-rev |
|
文獻引用方法 |
|
| 材料和方法部分: | pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT was a gift from Eric Fearon (Addgene plasmid # 19291) |
| 參考文獻部分: | The SLUG zinc-finger protein represses E-cadherin in breast cancer. Hajra KM, Chen DY, Fearon ER. Cancer Res. 2002 Mar 15. 62(6):1613-8. PubMed 11912130 |
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