LOCUS       pGM1190                6973 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  OriT containing mobilizable vector for thiostrepton induced gene 
            expression in Streptomyces.
ACCESSION   .
VERSION     .
KEYWORDS    pGM1190
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6973)
  TITLE     pGM1202, a mobilizable vector for gene expression in Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     oriT            467..576
                     /note="incP origin of transfer"
     CDS             892..1692
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2870..2891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2906..2936
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2944..2960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2949..2971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2968..2984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2968..2984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      3163..3197
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     misc_feature    3368..3424
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      3572..3620
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             5335..6777
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGRLPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
      181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
      241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
      301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
      361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
      421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
      481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
      541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
      601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
      661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
      721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
      901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
      961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
     1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
     1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
     1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
     3001 acgtaggcgt cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg
     3061 actggattcc aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg
     3121 acatggctcg attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg
     3181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg
     3241 ggatctgggc tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc
     3301 cgggcttgca cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg
     3361 acatatgaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
     3421 attccccaga tctaaagttt tgtcgtcttt ccagacgtta gtaaatgaat tttctgtatg
     3481 aggttttgct aaacaacttt caacagtttc agcggagtga gaatagaaag gaacaactaa
     3541 aggaattgcg aataataatt ttttcacgtt gaaaatctcc aaaaaaaaag gctccaaaag
     3601 gagcctttaa ttgtatcggt ttatcagctt gctttcgagg tgaatttctt aaacagcttg
     3661 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
     3721 gtcgctgagg cttgcaggga gtcaaaggcc gcttttgcgg gatctcgtcg aaggcactag
     3781 aggcctatat gcggggatcg accgcgcggg tcccggacgg ggaagagcgg ggagctttgc
     3841 cagagagcga cgacttcccc ttgcgttggt gattgccggt cagggcagcc atccgccatc
     3901 gtcgcgtagg gtgtcacacc ccaggaatcg cgtcactgaa cacagcagcc ggtaggacga
     3961 ccatgactga gttggacacc atcgcaaatc cgtccgatcc cgcggtgcag cggatcatcg
     4021 atgtcaccaa gccgtcgcga tccaacataa agacaacgtt gatcgaggac gtcgagcccc
     4081 tcatgcacag catcgcggcc ggggtggagt tcatcgaggt ctacggcagc gacagcagtc
     4141 cttttccatc tgagttgctg gatctgtgcg ggcggcagaa cataccggtc cgcctcatcg
     4201 actcctcgat cgtcaaccag ttgttcaagg gggagcggaa ggccaagaca ttcggcatcg
     4261 cccgcgtccc tcgcccggcc aggttcggcg atatcgcgag ccggcgtggg gacgtcgtcg
     4321 ttctcgacgg ggtgaagatc gtcgggaaca tcggcgcgat agtacgcacg tcgctcgcgc
     4381 tcggagcgtc ggggatcatc ctggtcgaca gtgacatcac cagcatcgcg gaccggcgtc
     4441 tccaaagggc cagccgaggt tacgtcttct cccttcccgt cgttctctcc ggtcgcgagg
     4501 aggccatcgc cttcattcgg gacagcggta tgcagctgat gacgctcaag gcggatggcg
     4561 acatttccgt gaaggaactc ggggacaatc cggatcggct ggccttgctg ttcggcagcg
     4621 aaaagggtgg gccttccgac ctgttcgagg aggcgtcttc cgcctcggtt tccatcccca
     4681 tgatgagcca gaccgagtct ctcaacgttt ccgtttccct cggaatcgcg ctgcacgaga
     4741 ggatcgacag gaatctcgcg gccaaccgat aagcgcctct gttcctcgga cgctcggttg
     4801 ctcgacctcg attcgtcagt gatgatcaac aggacccaga aatggcacga gcccgggatt
     4861 ggcctcccgg gctcgccgcc cccgaaccgg cgcagcatgc cgagtgagga cgtttgctct
     4921 gtgacgagcg tacgagagaa cagcgcgtcg acaccaccgc gccccgccgg accgaagtgc
     4981 cactgcggca ccccgatcgt ccacatcccc gggaagcgcc ccaaaatcta ctgctcggac
     5041 gcctgccgaa agcgcgcaaa gcgtctcatt gcggaccagt cacgcagcgt agcaagcacg
     5101 actacagcgg gtaatccgga cgcgggcttt agtagtaagg gaaaatctcc gtccactgca
     5161 ggtcagagcg ctgtaactgg aacgggttct accccaaacg agggtgccgc gacagccggg
     5221 cgggaggacc cgcgcgacga gcggttcgcc cggcgggacc ggcaccagac ggtgtcgctg
     5281 aacgaggcgt tcaagggctg cgggacccgg ctcactgccg ggacggccga gctgatgtgg
     5341 aagccgggcg aggcgacgtg gggcaacacg tgccgctgca acaacgtcca cacctgcccg
     5401 tggtgcatga gccgcatcct ggccgtacgg ggctcgaacg tgcagctcgc cgccgacggt
     5461 ctcgcggacg ccgggtacgg gctgcacctt ggcacgaaca cgttgcgcca cttcgagcgc
     5521 atggcgttcg gcaccgtccg caagggaatg cggcacggcc tggtcgcggt cttgcacgac
     5581 ggctggaagg gcgcgtacgg ctcgtccggc cgccgctggc gcacgatgcg cgacgacttc
     5641 gggatcatcg gctacgagcg ggccttcgag gacaccttcg gctggggctc cggctggcac
     5701 ctgcactggc acacgctctg ggtcacccgc gaggtgctcg gcccggacgc ccaggccgcg
     5761 ttccgcgacg cactcgccgg agcctgggcc gccggggtcg aatccgcggg cggctacacc
     5821 gtcagcgaga cgtgcgaccg gcccggttgc tcctgtgagg gcaagggcca cggcaccgac
     5881 gtgcgcccac tcaacggcgc ggacgccgct gacggcgatg cgggcaagca ggcccgctac
     5941 ctctacaagg acggcgacaa gaccaagggc ggcgtcgcca agatcggcct ggagctggcc
     6001 ggacagaact tcaaggccgg tcgcggcgac gaccgcatgg gaccgctcga cctgggcgac
     6061 gcggcggccg ccgagctgca gcggcttcgt cggcccggcc cgttcgtcga gaagtaccgc
     6121 gagcgcgagt tcggcgtctt ccaggtccgc aagcactacc ggtcgcagaa cctgaaccgg
     6181 ctgatcaagg aactcggcat ccagcaggac gtccgcaccg aggaggagat caccgacgac
     6241 accgagggcc tggtcgccat cgcggtcatc ccggcctaca tctggtaccg atacatcgcc
     6301 cgcgtcgccg gacgtcgcct cgacctcatc aaggtcgccg agacgtacgg cctaccgggc
     6361 gtccggcggc tcgtcgagtc ctggggcctg gtgtggggca aggacgtcct ggacccgccc
     6421 gccccggaag cgccggccgc gccgggcgac cttgacgctg accagatgag gttcgaggtc
     6481 atgtccgagg aggaagctgc tttccgcgag gcgcgccgga aggccaacga ggcccgcacg
     6541 gaggagctgg ccgcttcgct cgaccgagtg cgtcagccga agaaggaggc gatccgcccg
     6601 acgatcagcc ttcggaagcg cctgaagccg aagccggtca ccgtcgacgt caagactccc
     6661 ccgcccggcg ccgcgtcgcc cgtgtgccgc cgctgcaagg gcaagctcgc cccggtactc
     6721 cagccctggg gccggctccc cggagactgc ctccgcgtcg acacagcggt cgcgtagggc
     6781 cgctgaggga ccgccatgag accgccaccc cggacatccg ggtggcggtc tcatggcggt
     6841 cccggcgcga aagccgaggg tctgcctgcc gtgaggtggc cggcggacct gggttactgc
     6901 tggaatcccg cgcccagtcc gagcgtccgc cggcgctttt cacctggctc cgggtgctcg
     6961 tcgatgagca cca
//