| 質粒名稱: | pHDM-Tat1b |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 164442 |
| 存儲實驗室: | Alejandro Balazs |
|
載體骨架基本信息 |
|
| 載體名稱: | HDM |
| 載體出品公司: | -- |
| 空載體大小: | -- |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | -- |
| 載體抗性: | 氨芐霉素100 μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | HIV-1 Tat |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子: | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位點: | -- |
| 3’克隆位點: | -- |
| 5’測序引物 | -- |
| 3’測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pHDM-Tat1b was a gift from Alejandro Balazs (Addgene plasmid # 164442 ; //n2t.net/addgene:164442 ; RRID:Addgene_164442) |
| 參考文獻部分: | COVID-19-neutralizing antibodies predict disease severity and survival. Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB. Cell. 2021 Jan 21;184(2):476-488.e11. doi: 10.1016/j.cell.2020.12.015. Epub 2020 Dec 15. 10.1016/j.cell.2020.12.015 PubMed 33412089 |
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pHDM-Tat1b載體 pHDMTat1b質粒基本信息
pHDM-Tat1b質粒圖譜 pHDMTat1b載體圖譜和pHDM-Tat1b載體序列 pHDMTat1b質粒序列多克隆位點信息
pHDM-Tat1b質粒 pHDMTat1b載體簡介
pHDM-Tat1b質粒序列 pHDMTat1b載體序列
LOCUS pHDM-Tat1b 4829 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Encodes HIV-1 Tat accessory protein for pseudovirus production.
ACCESSION .
VERSION .
KEYWORDS pHDM-Tat1b
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4829)
TITLE COVID-19 neutralizing antibodies predict disease severity and
survival
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4829)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..4829
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(523..539)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(524..540)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 633..1027
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
primer_bind complement(1361..1379)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1447..1551
/gene="bla"
/label=AmpR promoter
CDS 1552..2412
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1770..1789)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2583..3171
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3072..3091
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(3714..3733)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 3835..4214
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4215..4418
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4369..4389
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4415..4439
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 4532..178
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
ORIGIN
1 tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca
61 gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct
121 gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagct
181 cctgggcaac gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat tccgcgggcg
241 gccgcgaaat ggagccagta gatcctagac tagagccctg gaagcatcca ggaagtcagc
301 ctaaaactgc ttgtaccact tgctattgta aaaagtgttg ctttcattgc caagtttgtt
361 tcacaacaaa agccttaggc atctcctatg gcaggaagaa gcggagacag cgacgaagac
421 ctcctcaagg cagtcagact catcaagttt ctctatcaaa gcaacccacc tcccaacccc
481 gaggggaccc gacaggcccg aaggaatagg atccaagctt atcgataccg tcgacctcga
541 gggcccagat ctaattcacc ccaccagtgc aggctgccta tcagaaagtg gtggctggtg
601 tggctaatgc cctggcccac aagtatcact aagctcgctt tcttgctgtc caatttctat
661 taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt
721 gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat
781 tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag
841 aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa
901 ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc
961 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg
1021 tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc
1081 ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt
1141 cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca
1201 gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat
1261 ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc
1321 cctcgacatg gcagtctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
1381 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
1441 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
1561 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
1621 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
1681 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
1741 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
1801 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
1861 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
1921 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
1981 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
2041 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
2101 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
2461 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
2521 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
2701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
3181 aacggagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
3241 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
3301 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
3361 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc
3421 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg
3481 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca
3541 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
3601 agaatcataa tggggaaggc catccagcct cgcgtcgggg agctttttgc aaaagcctag
3661 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
3721 ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc
3781 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt
3841 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
3901 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
3961 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
4021 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
4081 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
4141 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
4201 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
4261 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
4321 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
4381 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
4441 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
4501 tcccctcgaa gctgatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct
4561 ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca
4621 catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt
4681 aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca
4741 ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc
4801 tgggttaagg caatagcaat atttctgca
//
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