LOCUS       pJYS2_crtYf                4380 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  Constitutive transcription of crRNA of crtYf, Spectinomycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    pJYS2_crtYf
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, 
            Li Y, Wang R, Yang S
  TITLE     CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
  JOURNAL   Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
  PUBMED    28469274
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|gt12365.cn|
COMMENT     VNTNAME|pJYS2_crtYf|
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      58..143
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      3466..4054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3955..3974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4208..4225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      4319..4346
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
       61 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
      121 cgactgagcc tttcgtttta tttaaatttc ctgccctatg gttgcctgat ctacaacagt
      181 agaaattcgg atccattata cctaggactg agctagctgt caatctagac tcgaggagat
      241 ttttgagggg gagggagtcg aggaagagcc agagcagaag gcgggggaac cgttctctgc
      301 cgacagcgtg agccccctta aaaatcaggc cggggaggaa ccggggaggg atcagagcta
      361 ggagcgagac accctaaagg gggggaaccg ttttctgctg acggtgtttc gtttattagt
      421 tttcagcccg tggatagcgg agggtgaggg caagtgagag ccagagcaag gacgggaccc
      481 ctaaaggggg gaaccgtttt ctgctgacgg tgtttcgttt attagttttc agcccgtgga
      541 cggccgcgtt tagcttccat tccaagtgcc tttctgactt gttggatgcg cctttcactg
      601 acacctagtt cgcctgcaag ctcacgagtc gagggatcag caaccgattg agaacgggca
      661 tccaggatcg cagttttgac gcgaagttcg agcaactcgc ctgtcatttc tcggcgtttg
      721 tttgcttccg ctaatcgctg tcgcgtctcc tgcgcatact tactttctgg gtcagcccat
      781 ctgcgtgcat tcgatgtagc tgcgccccgt cgccccatcg tcgctagagc tttccgccct
      841 cggctgctct gcgtttccac ccgacgagca gggacgactg gctggccttt agccacgtag
      901 ccgcgcacac gacgcgccat cgtcaggcga tcacgcatgg cgggaagatc cggctcccgg
      961 ccgtctgcac cgaccgcctg ggcaacgttg tacgccactt catacgcgtc gatgatcttg
     1021 gcatctttta ggcgctcacc agcagctttg agctggtatc ccacggtcaa cgcgtggcga
     1081 aacgcggtct cgtcgcgcgc tgcgcgctct ggatttgtcc agagcactcg cacgccgtcg
     1141 atcaggtcgc cggacgcgtc cagggcgctc ggcaggctcg cgtccaaaat cgctagcgcc
     1201 ttggcttctg cggtggcgcg ttgtgccgct tcaatgcggg cgcgtccgct ggaaaagtcc
     1261 tgctcaatgt actttttcgg cttctgtgat ccggtcatcg ttcgagcaat ctccattagg
     1321 tcggccagcc gatccacacg atcatgctgg cagtgccatt tataggctgt cggatcgtct
     1381 gagacgtgca gcggccaccg gctcagccta tgcgaaaaag cctggtcagc gccgaaaaca
     1441 cgagtcattt cttccgtcgt tgcagccagc aggcgcatat ttgggctggt tttacctgct
     1501 gcggcataca ccgggtcaat gagccagatg agctggcatt tcccgctcag cggattcacg
     1561 ccgatccaag ccggcgcttt ttctaggcgt gcccatttct ctaaaatcgc gtagacctgc
     1621 gggtttacgt gctcaatctt cccgccggcc tggtggctgg gcacatcgat gtcaagcacg
     1681 atcaccgcgg catgttgcgc gtgcgtcagc gcaacgtact ggcaccgcgt cagcgctttt
     1741 gagccagccc ggtagagctt tggttgggtt tcgccggtat ccgggttttt aatccaggcg
     1801 ctcgcgaaat ctcttgtctt gctgccctgg aagctttcgc gtcccaggtg agcgagcagt
     1861 tcgcggcgat cttctgccgt ccagccgcgt gagcgcagcg ctagttcggg gtgggtgtcg
     1921 aacagatcgg cggaaaattt ccacgcgcta gctgtgactg tgtcctgcgg atcggctaga
     1981 gtcatgtctt gagtgctttc tcccagctga tgactggggg ttagccgacg ccctgtgagt
     2041 tcccgctcac ggggcgttca actttttcag gtatttgtgc agcttatcgt gttttcttcg
     2101 taaatgaacg cttaactacc ttgttaaacg tggcaaatag gcaggattga tggctttcat
     2161 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     2221 tttcgaaggg caccaataac tgccttaaaa aaagatctgt acatcgatat catatgcaag
     2281 ggtttattgt tttctaaaat ctgattacca attagaatga atatttccca aatattaaat
     2341 aataaaacaa aaaaattgaa aaaagtgttt ccaccatttt ttcaattttt ttataatttt
     2401 tttaatctgt tatttaaata gtttatagtt aaatttacat tttcattagt ccattcaata
     2461 ttctctccaa gataactacg aactgctaac aaaattctct ccctatgttc taatggagaa
     2521 gattcagcca ctgcatttcc cgcaatatct tttggtatga ttttacccgt gtccatagtt
     2581 aaaatcatac ggcataaagt taatatagag ttggtttcat catcctgata attatctatt
     2641 aattcctctg acgaatccat aatggctctt ctcacatcag aaaatggaat atcaggtagt
     2701 aattcctcta agtcataatt tccgtatatt cttttatttt ttcgttttgc ttggtaaagc
     2761 attatggtta aatctgaatt taattccttc tgaggaatgt atccttgttc ataaagctct
     2821 tgtaaccatt ctccataaat aaattcttgt ttgggaggat gattccacgg taccatttct
     2881 tgctgaataa taattgttaa ttcaatatat cgtaagttgc ttttatctcc tatttttttt
     2941 gaaataggtc taattttttg tataagtatt tctttacttt gatctgtcaa tggttcagat
     3001 acgacgacta aaaagtcaag atcactattt ggttttagtc cactctcaac tcctgatcca
     3061 aacatgtaag taccaataag gttatttttt aaatgtttcc gaagtatttt tttcacttta
     3121 ttaatttgtt cgtatgtatt caaatatatc ctcctcacta ttttgattag tacctatttt
     3181 atatccatag ttgttaatta aataaactta atttagttta tttatagatt tcattggctt
     3241 ctaaattttt tatctagata ataattattt tagttaattt tattctagat tatatatgat
     3301 atgatctttc atttccataa aactaaagta agtgtaaacc tattcattgt tttaaaaata
     3361 tctcttgcca gtcacgttac gttattagtt catgaccaaa atcccttaac gtgagttttc
     3421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3601 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4081 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4141 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4201 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4261 tacgcatctg tgcggtattt cacaccgcat atgaagctta agagtttgta gaaacgcaaa
     4321 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
//