LOCUS pLMB509 6879 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLMB509
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6879)
AUTHORS Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS
TITLE Regulatable vectors for environmental gene expression in
alphaproteobacteria.
JOURNAL Appl Environ Microbiol. 2012 Oct;78(19):7137-40. Epub 2012 Jul 20.
PUBMED 22820336
REFERENCE 2 (bases 1 to 6879)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//gt12365.cn
FEATURES Location/Qualifiers
source 1..6879
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 171..884
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(201..229)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 824..844
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
CDS 891..908
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 942..1028
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1717..2379)
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pBBR1 from Bordetella bronchiseptica"
/label=pBBR1 Rep
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
rep_origin 2380..3149
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS complement(4432..4965)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(5154..5182)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
ORIGIN
1 tgttcacctc gcccaactgg actcatctaa cttcctatct ggatataagg caatccgaag
61 cgtgggccta tttctattca gcaagcaaag accgtcattc aatgtacggc gcgaccccgc
121 aaaaagcggg gcgacataac caaagagggt aacgacagga ggaagaacat atgcgtaaag
181 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
241 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
301 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
361 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacgg catgactttt
421 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
481 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
541 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
601 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
661 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
721 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
781 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
841 taacagctgc tgggattaca catggcatgg atgaactata caaacatatg catcatcacc
901 atcatcacta agagctctac aaataatgaa ttcccaggca tcaaataaaa cgaaaggctc
961 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
1021 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
1081 caggacgccc gccataaact gccagggaat tccaactgag cgccggtcgc taccattacc
1141 aacttgtctg gtgtcaaaaa taataggatt ttgttaaaat tcgcgttaaa tttttgttaa
1201 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
1261 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
1321 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
1381 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
1441 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
1501 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
1561 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
1621 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
1681 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
1741 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
1801 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
1861 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
1921 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
1981 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
2041 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
2101 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
2161 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
2221 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
2281 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
2341 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
2401 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
2461 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
2521 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
2581 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
2641 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
2701 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
2761 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
2821 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
2881 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
2941 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
3001 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
3061 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
3121 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
3181 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
3241 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
3301 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
3361 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
3421 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
3481 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
3541 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
3601 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
3661 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
3721 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
3781 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
3841 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
3901 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
3961 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
4021 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
4081 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
4141 aaggccgatc aagcccaaga gacggcccga gaccttcggg agcgcctgaa gcccgttctg
4201 gacgccctgg ggccgttgaa tcgggatatg caggccaagg ccgccgcgat catcaaggcc
4261 gtgggcgaaa agctgctgac ggaacagcgg gaagtccagc gccagaaaca ggcccagcgc
4321 cagcaggaac gcgggcgcgc acatttcccc gaaaagtgcc acctggcggc gttgtgacaa
4381 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
4441 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
4501 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
4561 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
4621 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag
4681 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
4741 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
4801 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
4861 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
4921 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
4981 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
5041 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
5101 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
5161 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
5221 catgggcaaa tgggattggc tttactaagc tgatccggtg gatgaccttt tgaatgacct
5281 ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa
5341 attttttcac aaaacggttt acaagcataa agcttgctca atcaatcacc ggatctagat
5401 atcgataccc cgctccgcga tccgcgccgc agtcagggcc acgccgtcgg ctatgcggtt
5461 gcgcgcgatc gacgagtagc ccatgcggaa gaagcggcat gggccgtcgt ccttcgggaa
5521 gaagggcgac ccggattcga tcagcacgcc gtcctttcgc aaagcgttca tcagcaagtc
5581 cgcatcgaga ccttccggcc cctcgatcca gaaggacgtg ccgccgaaag ccgcagatcc
5641 ggcgatctcc aatccggcat cccggagggc agcgtccatg atgatgtggc ggcgatgata
5701 ctcctcgcgc atgcggtgca gcacggcatc atagtgtccg agcgccagga aataggcggc
5761 cgttctctgg aggtgccccg gcggatgccg caacatcaag gcccgtagtg cccgcgcctc
5821 ccggatcacg gcagccggcg ccaccagata gccgagacgc aggccgggaa acaacgactt
5881 ggagaaactg ccgatgtaga agacccggcc gcagcggtcg aaggccttga gggccgggga
5941 ggggggcgcc agaaagctca tctcgaactc gtaatcgtcc tcgacgatta tgaagtcctt
6001 ggaggcggcc gcctcgagca aggccgcccg ccgttcgatc ggcatcgtcg cgccggtcgg
6061 cgaatgatgg ctgggcgtga cgaacacggc atcgacctct tctggaagcg cggcgggcgg
6121 caggccgtcc ttgtccacgt cgacgaaggt gatcccggcg ccgctcaagc gaagcgatgc
6181 actcatgtcg ggatggcagg ggttctcgca gaccgcatgg gcgccctgac gcaacaggag
6241 ctggatgacg atccagaggg cgttctgcgc gcccaccgtc accaggatct cgtcggggtt
6301 cgcgcggatg ccgcggcccg gcaatgtgcg cgagcagata tagttgacaa gctggatgtc
6361 gtccgccgcc gcgaagtcgc ccgccatcag ctcgaaatcc tcgcgcgcca gcgcccgtcg
6421 cgcgcagtcg cgccaggccg tgaggtcgaa cagcgacggg tccatctgac cgtataggaa
6481 cggataaggg taggttcgcc agtcgagggg cttacgcatc tgcttggcca cgatgaaggt
6541 cgagcgcaac ttggccgacc agtcgatggc atcggccgca gcatgggcgg cctgcatcgc
6601 gatggctgtc gttgggggct tgccggccac acggtagccg ctccggttcg ccgcctccac
6661 atagccctgg gaagcgagtt cctgataggc gagcgttacg gtgatccgcg aaatattcag
6721 ataggccgca agcttgcggc tggagggcaa ttgggcgccc ggcgccaggc ggcccgacaa
6781 tacggccgaa acgatcgttt cgcggatctg cgcctgcagg cccgtttggc tggcccggtc
6841 aaggaaaaag atcgtttcgg agacggtcga gcccgccat
//
