| 質粒名稱: | pLOVE-Oct4 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 15952 |
| 存儲實驗室: | Miguel Ramalho-Santos |
|
載體骨架基本信息 |
|
| 載體名稱: | pLOVE |
| 載體出品公司: | Clontech |
| 空載體大小: | 8263 bp |
| 完整質粒大小: | 8263 bp |
| 載體類型: | 哺乳細胞表達, Lentiviral |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | POU domain, class 5, transcription factor 1 (Oct4) / POU5F1, OTF3 |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 1398 bp |
| 基因突變: | The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Oct4 is ~8205 bp. |
| 標簽/融合蛋白: | V5 (C terminal on insert) |
| 克隆方法: | 限制性內切酶 |
| 5’測序引物 | n/a |
| 3’測序引物 | V5 |
|
文獻引用方法 |
|
| 材料和方法部分: | pLOVE-Oct4 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15952) |
| 參考文獻部分: | Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358 |
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pLOVE-Oct4載體質粒基本信息
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pLOVE-Oct4質粒序列載體序列
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