LOCUS       pLV-EF1a-MinD-IRES-Puro                9512 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  Expression of untagged MinD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-EF1a-MinD-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
  TITLE     A programmable reaction-diffusion system for spatiotemporal cell 
            signaling circuit design.
  JOURNAL   Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 
            10.1016/j.cell.2023.12.007.
  PUBMED    38181787
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2024 from SnapGene Server 1.1.58
            //gt12365.cn
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        83..294
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          295..1233
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1190..1210
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1249..1258
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(2312..2329)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2539..2558
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2720..3319
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2720..2739)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3216..3236
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3343..3931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3396..3416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3814..3825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4138..4771
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4899..4915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4899..4915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4912..4934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4923..4939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4947..4977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4992..5013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5130..5147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5301..5889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5381..5400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6060..6920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6683..6702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6921..7025)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7073..7207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7110..7129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7164..7183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7375..8008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8055..8180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8677..8910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9095..9139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9402..5
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 aattttcggg tttattacag ggacagcaga gatccagttt atcgatgagg ccctttcgtc
       61 ttcactcgag gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      121 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      181 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      241 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      301 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      361 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      421 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      481 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      541 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      601 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      661 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      721 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      781 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      841 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      901 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      961 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1021 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1081 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1141 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1201 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatccgc caccatggcc
     1261 cgcataatag ttgtgacttc aggtaagggc ggagttggaa aaaccacatc cagcgcagcc
     1321 atagctaccg gcctcgcaca gaaaggcaag aaaactgtgg tcatagattt tgatattggg
     1381 ctcaggaacc tggacctgat aatgggctgc gaacggcgcg ttgtctacga ttttgtcaat
     1441 gtcatccaag gcgacgcgac tcttaatcag gcacttataa aagataaacg aaccgaaaac
     1501 ttgtatattc ttcctgcctc acaaacgcgg gacaaagacg cgctcaccag ggagggtgtc
     1561 gcaaaagttt tggatgactt gaaagcgatg gactttgagt tcatagtttg tgactctccc
     1621 gccggtatag agacaggggc acttatggca ctgtacttcg cagatgaagc cataatcaca
     1681 actaatccgg aggtgtctag tgtgcgagat tcagaccgaa tactggggat actcgccagc
     1741 aaatcacgga gggcagaaaa cggggaggag ccaattaagg agcatctcct gcttacccga
     1801 tataaccccg gtcgagtatc tcggggagat atgctttcaa tggaagatgt cctcgagatt
     1861 ctgaggataa aactggtagg cgttatcccg gaagaccagt ccgtcctccg cgcatctaat
     1921 cagggggagc ctgtcatact tgacataaat gcggatgctg ggaaagctta tgccgatacg
     1981 gtagaacggc tcctcgggga agaacggccc ttccgcttca tagaggagga aaagaaggga
     2041 ttccttaaac ggctgttcgg gggataagcg gccgctctag agtcgacggg ccgcggtaac
     2101 aattgttaac taacttaagc tagcaacggt ttccctctag cgggatcaat tccgcccccc
     2161 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2221 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2281 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2341 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2401 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2461 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2521 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2581 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     2641 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2701 tgaaaaacac gataatacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     2761 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     2821 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     2881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     2941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3301 ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc tctggattac
     3361 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3721 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3901 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     3961 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     4021 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     4081 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     4141 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     4201 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     4261 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     4321 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     4381 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     4441 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     4501 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     4561 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     4621 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4681 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4741 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     4801 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     4861 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     4921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7021 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
     7081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     7261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     7321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     7381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     7441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     7741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     7801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     9361 cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga
     9421 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9481 aagaattaca aaaacaaatt acaaaaattc aa
//