| 質粒名稱: | pLXRN |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 99206 |
| 存儲實驗室: | Peter Pedersen |
|
載體骨架基本信息 |
|
| 載體名稱: | pLXSN |
| 載體出品公司: | Dr. Dusty Miller, Fred Hutchinson Cancer Center, Seattle, WA |
| 載體抗性: | 氨芐青霉素 |
| 載體修飾: | 將SV40啟動子驅動的新霉素選擇標記物替換為腦心肌炎病毒的內部核糖體進入位點/序列（IRES），生成雙順反子逆轉錄病毒載體 |
| 載體類型: | 哺乳動物表達，逆轉錄病毒 |
| 篩選標記: | 新霉素（G418） |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 啟動子 | LTR |
| 克隆方法: | 限制性內切酶 |
| 5' 測序引物 | CCCTTGAACCTCCTCGTTCGACC |
| 3' 測序引物 | GTAAAGCATGTGCACCGAGGCC |
|
文獻引用方法 |
|
| 材料和方法部分: | pLXRN was a gift from Peter Pedersen (Addgene plasmid # 99206 ; //n2t.net/addgene:99206 ; RRID:Addgene_99206) |
買家導航
pLXRN載體質粒基本信息
pLXRN質粒圖譜載體圖譜和pLXRN載體序列質粒序列多克隆位點信息
pLXRN質粒載體簡介
pLXRN質粒序列載體序列
LOCUS pLXRN 6091 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bicistronic retroviral vector.
ACCESSION .
VERSION .
KEYWORDS pLXRN
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6091)
TITLE pLXRN bicistronic retroviral vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6091)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..6091
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 11..29
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
LTR 631..806
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 869..1068
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1269..1685
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1615..1637
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1653..1669
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1710..2281
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1875..1892)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2102..2121
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2317..3120
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2380..2399)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 2990..3009
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 3180..3773
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(3876..3898)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3984..4124
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4039..4058
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4139..4156)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4310..4898)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4390..4409)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5069..5929)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5692..5711
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5930..6034)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgc tagcaattgc
61 tagcaattgc tagcaattca taccagatca ccgaaaactg tcctccaaat gtgtccccct
121 cacactccca aattcgcggg cttctgcctc ttagaccact ctaccctatt ccccacactc
181 accggagcca aagccgcggc ccttccgttt ctttgctttt gaaagacccc acccgtaggt
241 ggcaagctag cttaagtaac gccactttgc aaggcatgga aaaatacata actgagaata
301 gaaaagttca gatcaaggtc aggaacaaag aaacagctga ataccaaaca ggatatctgt
361 ggtaagcggt tcctgccccg gctcagggcc aagaacagat gagacagctg agtgatgggc
421 caaacaggat atctgtggta agcagttcct gccccggctc ggggccaaga acagatggtc
481 cccagatgcg gtccagccct cagcagtttc tagtgaatca tcagatgttt ccagggtgcc
541 ccaaggacct gaaaatgacc ctgtacctta tttgaactaa ccaatcagtt cgcttctcgc
601 ttctgttcgc gcgcttccgc tctccgagct caataaaaga gcccacaacc cctcactcgg
661 cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt
721 gctgtttgca tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
781 actacccacg acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc
841 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
901 attgtctagt gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc
961 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
1021 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
1081 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
1141 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
1201 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
1261 aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
1321 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
1381 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
1441 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
1501 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
1561 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
1621 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
1681 gcgccggaat tcgttaactc gaggatccgc ccctctccct ccccccccta acgttactgg
1741 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
1801 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
1861 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
1921 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
1981 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
2041 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
2101 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
2161 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
2221 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
2281 agcttgccac aacccgggat aattcctgca gccaatatgg gatcggccat tgaacaagat
2341 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
2401 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
2461 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
2521 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
2581 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
2641 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
2701 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
2761 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
2821 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc
2881 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
2941 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
3001 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
3061 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
3121 gcgggactct ggggttcgat aaaataaaag attttattta gtctccagaa aaagggggga
3181 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
3241 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
3301 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
3361 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
3421 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
3481 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
3541 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
3601 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
3661 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
3721 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
3781 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct
3841 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
3901 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
3961 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
4021 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
4081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
4141 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
4201 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4741 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
5401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
5701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6061 acgtctaaga aaccattatt atcatgacat t
//
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