| 質粒名稱: | pLenti-V5-KDM3A |
|---|---|
| 出品公司： | addgene |
| 目錄編號: | 82331 |
| 存儲實驗室: | Luke Gaughan |
|
載體骨架基本信息 |
|
| 載體名稱: | pLenti6 |
| 空載體大小: | 4038 bp |
| 完整質粒大小: | 4989 bp |
| 載體類型: | 哺乳細胞表達、Lentiviral |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | KDM3A |
| 物種來源: | H. sapiens (human) |
| 基因突變: |
wo silent mutations to make it siRNA resistant
Entrez GeneKDM3A (a.k.a. JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA)
|
| 標簽/融合蛋白: | V5 (C terminal on backbone) |
| 克隆方法: | Gateway Cloning |
| 5’測序引物 | CMV-F |
|
文獻引用方法 |
|
| 材料和方法部分: | pLenti-V5-KDM3A was a gift from Luke Gaughan (Addgene plasmid # 82331 ; //n2t.net/addgene:82331 ; RRID:Addgene_82331) |
| 參考文獻部分: | The histone demethylase enzyme KDM3A is a key estrogen receptor regulator in breast cancer. Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan L. Nucleic Acids Res. 2015 Jan;43(1):196-207. doi: 10.1093/nar/gku1298. Epub 2014 Dec 8. 10.1093/nar/gku1298 PubMed 25488809 |
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pLenti-V5-KDM3A載體質粒基本信息
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pLenti-V5-KDM3A質粒載體簡介
pLenti-V5-KDM3A質粒序列載體序列
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pLenti-V5-KDM3A質粒載體應用舉例
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