LOCUS       pMSCV-FlagMLL-pl-ENL(5613)               11106 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMSCV-FlagMLL-pl-ENL(5613)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Bach C, Mueller D, Buhl S, Garcia-Cuellar MP, Slany RK
  TITLE     Alterations of the CxxC domain preclude oncogenic activation of 
            mixed-lineage leukemia 2.
  JOURNAL   Oncogene. 2009 Feb 12. 28(6):815-23.
  PUBMED    19060922
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            //gt12365.cn
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1415..1438
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6074..6090)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6075..6091)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        6108..6607
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6143..6166)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6540..6559
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6621..7424
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6684..6703)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7294..7313
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7499..8013
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8175..8191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8199..8229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8244..8265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8382..8399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8553..9141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8633..8652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9312..10172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9935..9954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10173..10277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10345..10363
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10401..10423)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10523..10542
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10736..10758
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10886..10905
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             11105..515
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattc catggactac aaggacgacg atgacaaggg
     1441 caattccgcg aacatggcgc acagctgtcg gtggcgcttc cccgcccgac ccgggaccac
     1501 cgggggcggc ggcggcgggg ggcgccgggg cctagggggc gccccgcggc aacgcgtccc
     1561 ggccctgctg cttccccccg ggcccccggt cggcggtggc ggccccgggg cgcccccctc
     1621 ccccccggct gtggcggccg cggcggcggc ggcgggaagc agcggggctg gggttccagg
     1681 gggagcggcc gccgcctcag cagcctcctc gtcgtccgcc tcgtcttcgt cttcgtcatc
     1741 gtcctcagcc tcttcagggc cggccctgct ccgggtgggc ccgggcttcg acgcggcgct
     1801 gcaggtctcg gccgccatcg gcaccaacct gcgccggttc cgggccgtgt ttggggagag
     1861 cggcggggga ggcggcagcg gagaggatga gcaattctta ggttttggct cagatgaaga
     1921 agtcagagtg cgaagtccca caaggtctcc ttcagttaaa actagtcctc gaaaacctcg
     1981 tgggagacct agaagtggct ctgaccgaaa ttcagctatc ctctcagatc catctgtgtt
     2041 ttcccctcta aataaatcag agaccaaatc tggagataag atcaagaaga aagattctaa
     2101 aagtatagaa aagaagagag gaagacctcc caccttccct ggagtaaaaa tcaaaataac
     2161 acatggaaag gacatttcag agttaccaaa gggaaacaaa gaagatagcc tgaaaaaaat
     2221 taaaaggaca ccttctgcta cgtttcagca agccacaaag attaaaaaat taagagcagg
     2281 taaactctct cctctcaagt ctaagtttaa gacagggaag cttcaaatag gaaggaaggg
     2341 ggtacaaatt gtacgacgga gaggaaggcc tccatcaaca gaaaggataa agaccccttc
     2401 gggtctcctc attaattctg aactggaaaa gccccagaaa gtccggaaag acaaggaagg
     2461 aacacctcca cttacaaaag aagataagac agttgtcaga caaagccctc gaaggattaa
     2521 gccagttagg attattcctt cttcaaaaag gacagatgca accattgcta agcaactctt
     2581 acagagggca aaaaaggggg ctcaaaagaa aattgaaaaa gaagcagctc agctgcaggg
     2641 aagaaaggtg aagacacagg tcaaaaatat tcgacagttc atcatgcctg ttgtcagtgc
     2701 tatctcctcg cggatcatta agacccctcg gcggtttata gaggatgagg attatgaccc
     2761 tccaattaaa attgcccgat tagagtctac accgaatagt agattcagtg ccccgtcctg
     2821 tggatcttct gaaaaatcaa gtgcagcttc tcagcactcc tctcaaatgt cttcagactc
     2881 ctctcgatct agtagcccca gtgttgatac ctccacagac tctcaggctt ctgaggagat
     2941 tcaggtactt cctgaggagc ggagcgatac ccctgaagtt catcctccac tgcccatttc
     3001 ccagtcccca gaaaatgaga gtaatgatag gagaagcaga aggtattcag tgtcggagag
     3061 aagttttgga tctagaacga cgaaaaaatt atcaactcta caaagtgccc cccagcagca
     3121 gacctcctcg tctccacctc cacctctgct gactccaccg ccaccactgc agccagcctc
     3181 cagtatctct gaccacacac cttggcttat gcctccaaca atccccttag catcaccatt
     3241 tttgcctgct tccactgctc ctatgcaagg gaagcgaaaa tctattttgc gagaaccgac
     3301 atttaggtgg acttctttaa agcattctag gtcagagcca caatactttt cctcagcaaa
     3361 gtatgccaaa gaaggtctta ttcgcaaacc aatatttgat aatttccgac cccctccact
     3421 aactcccgag gacgttggct ttgcatctgg tttttctgca tctggtaccg ctgcttcagc
     3481 ccgattgttt tcgccactcc attctggaac aaggtttgat atgcacaaaa ggagccctct
     3541 tctgagagct ccaagattta ctccaagtga ggctcactct agaatatttg agtctgtaac
     3601 cttgcctagt aatcgaactt ctgctggaac atcttcttca ggagtatcca atagaaaaag
     3661 gaaaagaaaa gtgtttagtc ctattcgatc tgaaccaaga tctccttctc actccatgag
     3721 gacaagaagt ggaaggctta gtagttctga gctctcacct ctcacccccc cgtcttctgt
     3781 ctcttcctcg ttaagcattt ctgttagtcc tcttgccact agtgccttaa acccaacttt
     3841 tacttttcct tctcattccc tgactcagtc tggggaatct gcagagaaaa atcagagacc
     3901 aaggaagcag actagtgctc cggcagagcc attttcatca agtagtccta ctcctctctt
     3961 cccttggttt accccaggct ctcagactga aagagggaga aataaagaca aggcccccga
     4021 ggagctgtcc aaagatcgag atgctgacaa gagcgtggag aaggacaaga gtagagagag
     4081 agaccgggag agagaaaagg agaataagcg ggagtcaagg aaagagaaaa ggaaaaaggg
     4141 atcagaaatt cagagtagtt ctgctttgta tcctgtgggt agggtttcca aagagaaggt
     4201 tgttggtgaa gatgttgcca cttcatcttc tgccaaaaaa gcaacagggc ggaagaagtc
     4261 ttcatcacat gattctggga ctgatattac ttctgtgact cttggggata caacagctgt
     4321 caaaaccaaa atacttataa agaaagggag aggaaatctg gaaaaaacca acttggacct
     4381 cggcccaact gccccatccc tggagaagga gaaaaccctc tgcctttcca ctccttcatc
     4441 tagcactgtt aaacattcca cttcctccat aggctccatg ttggctcagg cagacaagct
     4501 tccaatgact gacaagaggg ttgccagcct cctaaaaaag gccaaagctc agctctgcaa
     4561 gattgagaag agtaagagtc ttaaacaaac cgaccagccc aaagcacagg gtcaagaaag
     4621 tgactcatca gagacctctg tgcgaggacc ccggattaaa catgtctgca gaagagcagc
     4681 tgttgccctt ggccgaaaac gagctgtgtt tcctgatgac atgcccaccc tgagtgcctt
     4741 accatgggaa gaacgagaaa agattttgtc ttccatgggg aatgatgaca agtcatcaat
     4801 tgctggctca gaagatgctg aacctcttgc tccacccatc aaaccaatta aacctgtcac
     4861 tagaaacaag gcaccccagg aacctccagt aaagaaagga cgtcgatcga ggcggtgtgg
     4921 gcagtgtccc ggctgccagg tgcctgagga ctgtggtgtt tgtactaatt gcttagataa
     4981 gcccaagttt ggtggtcgca atataaagaa gcagtgctgc aagatgagaa aatgtcagaa
     5041 tctacaatgg atgccttcca aagcctacct gcagaagcaa gctaaagctg tgaaaaagaa
     5101 agagaaaaag tctaagacca gtgaaaagaa agacagcaaa gagagcagtg ttgtgaagaa
     5161 cgtggtggac tctagtcaga aacctacccc atcagcaaga gaggatcctg ccccaaagaa
     5221 aagcagtagt gagcctcctc cacgaaagcc cgtcgaggaa aagagtgaag aagggaatgt
     5281 ctcggcccct gggcctgaat ccaaacaggc caccactcca gcttccagga agtcaagcaa
     5341 gcaggtctcc cagccagcac tggtcatccc gcctcagcca cctactacag gaccgccaag
     5401 aaaagaagtt cccaaaacca ctcctagtga gcccaagaaa aagcagcctc caccaccaga
     5461 atcaggtcca gagcagagca aacagaaaaa agtggctccc cgcccaagta tccctgtaaa
     5521 acaaaaacca aaagaaaagg aaaaaccacc tccggtcaat aagcaggaga atgcaggcac
     5581 tttgaacatc ctcagcactc tctccaatgg caatagttct aagcaaaaaa ttccagcaga
     5641 tgggtcgcga agtatacaca cggggaggga ctccaggttg agcttcagcg acagcgagag
     5701 tgacaacagc gccgactcct ccctgcccag ccgtgagccc ccaccccccc agaagccacc
     5761 cccgcccaac agcaaggtgt caggccggag gagccccgag tcctgcagca agcctgagaa
     5821 gatcctcaag aagggcacct acgacaaggc ctacacggat gagctggtgg agctacaccg
     5881 gaggctgatg gcgctgcggg agcgcaacgt gctgcagcag attgtgaatc tgatcgagga
     5941 gactggccac ttcaatgtca ccaacaccac cttcgacttc gacctcttct ccctggacga
     6001 gaccaccgtg cgcaaactgc agagctgcct ggaggccgtg gccacatgac cctgaattcg
     6061 atatcaagct tatcgatacc gtcgacctcg agagatctaa ttctaccggg taggggaggc
     6121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     6181 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     6241 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     6301 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     6361 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     6421 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     6481 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     6541 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     6601 cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt
     6661 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6721 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6781 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6841 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6901 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6961 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7021 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7081 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7141 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7201 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7261 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7321 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7381 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat ccgtcgacct
     7441 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     7501 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     7561 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
     7621 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     7681 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
     7741 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     7801 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     7861 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
     7921 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     7981 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
     8041 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
     8101 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
     8161 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8221 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8281 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8341 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9601 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9661 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9721 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9781 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9841 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9901 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9961 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10021 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10081 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10141 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    10321 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
    10381 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10441 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10501 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    10561 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
    10621 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10681 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10741 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
    10801 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
    10861 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
    10921 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
    10981 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
    11041 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
    11101 ggggaa
//