| 質粒名稱: | pMSCVpuroATT |
|---|---|
| 出品公司: | addgene |
| 目錄編號: | 47539 |
| 存儲實驗室: | David Weinstock |
|
載體骨架基本信息 |
|
| 載體名稱: | pMSCVpuro |
| 載體出品公司: | Clontech |
| 完整質粒大小: | 6300bp |
| 載體類型: | 哺乳細胞表達、Retroviral |
| 篩選標記: | Puromycin |
| 載體抗性: | 氯霉素和氨芐西林 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 克隆方法: | Gateway Cloning |
| 5’測序引物 | pLXSN-5 |
| 3’測序引物 | MSCV-rev |
|
文獻引用方法 |
|
| 材料和方法部分: | pMSCVpuroATT was a gift from David Weinstock (Addgene plasmid # 47539 ; //n2t.net/addgene:47539 ; RRID:Addgene_47539) |
| 參考文獻部分: | Next-generation cDNA screening for oncogene and resistance phenotypes. Shindoh N, Yoda A, Yoda Y, Sullivan TJ, Weigert O, Lane AA, Kopp N, Bird L, Rodig SJ, Fox EA, Weinstock DM. PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub 2012 Nov 7. 10.1371/journal.pone.0049201 PubMed 23145123 |
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