LOCUS       pREP4-Luc               10257 bp ds-DNA     linear   SYN 02-MAY-2019
DEFINITION  This plasmid contains EBNA sequence..
ACCESSION   .
VERSION     .
KEYWORDS    pREP4-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Liu R, Liu H, Chen X, Kirby M, Brown PO, Zhao K
  TITLE     Regulation of CSF1 promoter by the SWI/SNF-like BAF complex.
  JOURNAL   Cell. 2001 Aug 10;106(3):309-18.
  PUBMED    11509180
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 2, 2019 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1269..3058
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     regulatory      3588..3597
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3594..5246
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3660..3678)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5287..5408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5298..5317)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5352..5371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    5932..5980
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6005..6024
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6022..7059)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(7102..7247)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(7678..8266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7758..7777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8437..9297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9060..9079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9298..9402)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9470..9488
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aggggcagga gcaggaggtg gaggccgggg tcgaggaggc agtggaggcc ggggtcgagg
       61 aggtagtgga ggccggggtc gaggaggtag tggaggccgc cggggtagag gacgtgaaag
      121 agccaggggg ggaagtcgtg aaagagccag ggggagaggt cgtggacgtg gagaaaagag
      181 gcccaggagt cccagtagtc agtcatcatc atccgggtct ccaccgcgca ggccccctcc
      241 aggtagaagg ccatttttcc accctgtagg ggaagccgat tattttgaat accaccaaga
      301 aggtggccca gatggtgagc ctgacgtgcc cccgggagcg atagagcagg gccccgcaga
      361 tgacccagga gaaggcccaa gcactggacc ccggggtcag ggtgatggag gcaggcgcaa
      421 aaaaggaggg tggtttggaa agcatcgtgg tcaaggaggt tccaacccga aatttgagaa
      481 cattgcagaa ggtttaagag ctctcctggc taggagtcac gtagaaagga ctaccgacga
      541 aggaacttgg gtcgccggtg tgttcgtata tggaggtagt aagacctccc tttacaacct
      601 aaggcgagga actgcccttg ctattccaca atgtcgtctt acaccattga gtcgtctccc
      661 ctttggaatg gcccctggac ccggcccaca acctggcccg ctaagggagt ccattgtctg
      721 ttatttcatg gtctttttac aaactcatat atttgctgag gttttgaagg atgcgattaa
      781 ggaccttgtt atgacaaagc ccgctcctac ctgcaatatc agggtgactg tgtgcagctt
      841 tgacgatgga gtagatttgc ctccctggtt tccacctatg gtggaagggg ctgccgcgga
      901 gggtgatgac ggagatgacg gagatgaagg aggtgatgga gatgagggtg aggaagggca
      961 ggagtgatgt aacttgttag gagacgccct caatcgtatt aaaagccgtg tattcccccg
     1021 cactaaagaa taaatcccca gtagacatca tgcgtgctgt tggtgtattt ctggccatct
     1081 gtcttgtcac cattttcgtc ctcccaacat ggggcaattg ggcataccca tgttgtcacg
     1141 tcactcagct ccgcgctcaa caccttctcg cgttggaaaa cattagcgac atttacctgg
     1201 tgagcaatca gacatgcgac ggctttagcc tggcctcctt aaattcacct aagaatggga
     1261 gcaaccagca ggaaaaggac aagcagcgaa aattcacgcc cccttgggag gtggcggcat
     1321 atgcaaagga tagcactccc actctactac tgggtatcat atgctgactg tatatgcatg
     1381 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt
     1441 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt
     1501 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     1561 aggatagcct atgctaccca gatatagatt aggatagcat atgctaccca gatatagatt
     1621 aggatagcat atgctatcca gatatttggg tagtatatgc tacccagata taaattagga
     1681 tagcatatac taccctaatc tctattagga tagcatatgc tacccggata cagattagga
     1741 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     1801 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga
     1861 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga
     1921 tagcatatgc tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc
     1981 gtgctctcag cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc
     2041 aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac
     2101 ctacttatgc aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc
     2161 gcagtggtta gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa
     2221 ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag
     2281 tggccacttg tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg
     2341 tgttagagac aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac
     2401 aaatagagtg taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata
     2461 accccagtat catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga
     2521 tggacatcca gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc
     2581 agaatgtttc attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg
     2641 tgtcatagca caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc
     2701 tgaaaccttg ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc
     2761 tattagctaa acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg
     2821 ctccttgatc ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc
     2881 tgaccccatg taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag
     2941 gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt
     3001 gaattagggt tagtctggat agtatatact actacccggg aagcatatgc tacccgttta
     3061 gggttaacaa gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc
     3121 ggtagctaca caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc
     3181 cctgggaggt acatgtcccc cagcattggt gtaagagctt cagccaagag ttacacataa
     3241 aggcaatgtt gtgttgcagt ccacagactg caaagtctgc tccaggatga aagccactca
     3301 gtgttggcaa atgtgcacat ccatttataa ggatgtcaac tacagtcaga gaaccccttt
     3361 gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag ttgtaccaac
     3421 caactgaagg gattacatgc actgccccgc gaagaagggg cagagatgtc gtagtcaggt
     3481 ttagttcgtc cggggcgggg atcgatcctc tagagtcgag atctggtacc agctgctagc
     3541 ggccgcgaag gcctcgagaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
     3601 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
     3661 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     3721 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
     3781 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     3841 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     3901 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     3961 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
     4021 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     4081 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
     4141 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
     4201 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
     4261 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     4321 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     4381 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
     4441 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
     4501 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     4561 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     4621 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     4681 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     4741 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     4801 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     4861 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     4921 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     4981 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     5041 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     5101 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     5161 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     5221 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
     5281 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5341 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5401 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5461 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     5521 ctagcttggc acgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc
     5581 cacagacgcc cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa
     5641 aaccatgggt ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata
     5701 ataaccccca cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc
     5761 cagtggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     5821 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     5881 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     5941 acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc
     6001 ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccgatccg gacgagtgct
     6061 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     6121 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     6181 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     6241 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
     6301 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     6361 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     6421 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     6481 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     6541 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     6601 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     6661 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
     6721 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     6781 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
     6841 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     6901 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     6961 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     7021 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc
     7081 tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg gtcgctcggt gttcgaggcc
     7141 acacgcgtca ccttaatatg cgaagtggac ctcggaccgc gccgccccga ctgcatctgc
     7201 gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat agaactaaag
     7261 acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg ggccacgggg
     7321 atgaagcagg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
     7381 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
     7441 ccatctccag cagccgcacg cggcgcatct cggggccgac gcgctgggct acgtcttgct
     7501 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     7561 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     7621 acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7681 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7741 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7801 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7861 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7921 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7981 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8041 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8101 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8161 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8221 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8281 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8341 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     8761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     9061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9421 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9481 cacgaggccc tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagctga
     9541 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
     9601 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
     9661 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
     9721 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
     9781 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
     9841 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
     9901 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
     9961 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
    10021 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
    10081 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
    10141 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
    10201 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcagg
//