| 質粒名稱: | pLV-mTurquoise2-Caspase-1-mNeonGreen |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 110770 |
| 存儲實驗室: | David Russell |
|
載體骨架基本信息 |
|
| 載體名稱: | pBluescript II KS+ |
| 載體出品公司: | Stratagene, La Jolla, CA |
| 空載體大小: | 3100bp |
| 完整質粒大小: | 7746bp |
| 載體類型: | AAV |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5alpha ; DH10B |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | AAV6 cap, AAV6 rep genes |
| 物種來源: | adeno-associated virus |
| 插入大小: | 4405bp |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | BamHI (not destroyed) |
| 3’克隆位點: | BamHI (not destroyed) |
| 5’測序引物 | 未知 |
| 3’測序引物 | 未知 |
|
文獻引用方法 |
|
| 材料和方法部分: | pRepCap6 was a gift from David Russell (Addgene plasmid # 110770 ; //n2t.net/addgene:110770 ; RRID:Addgene_110770) |
| 參考文獻部分: | Infectious clones and vectors derived from adeno-associated virus (AAV) serotypes other than AAV type 2. Rutledge EA, Halbert CL, Russell DW. J Virol. 1998 Jan;72(1):309-19. PubMed 9420229 |
買家導航
pRepCap6載體質粒基本信息
pRepCap6質粒圖譜載體圖譜和pRepCap6載體序列質粒序列多克隆位點信息
pRepCap6質粒載體簡介
pRepCap6質粒序列載體序列
LOCUS pRepCap6 7543 bp DNA circular SYN 14-APR-2025
DEFINITION pRepCap6 contains both rep and cap genes from the AAV6 genome, but
lacks the viral terminal repeats (Rutledge et al., 1998). It lacks
adenovirus helper functions required for stock production..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7543)
AUTHORS Rutledge EA, Halbert CL, Russell DW
TITLE Infectious clones and vectors derived from adeno-associated virus
(AAV) serotypes other than AAV type 2.
JOURNAL J Virol. 1998 Jan;72(1):309-19.
PUBMED 9420229
REFERENCE 2 (bases 1 to 7543)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Virol.";
date: "1998-01"; volume: "72(1)"; pages: "309-19"
COMMENT Sequence Label: pRepCap6
FEATURES Location/Qualifiers
source 1..7543
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 68..86
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(124..146)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 246..265
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(332..787)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(469..490)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 681..700
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 913..935
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 927..944
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 928..944
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 951..970
/label=T7
/note="T7 promoter, forward primer"
promoter 951..969
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(1258..1269)
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
promoter complement(5378..5396)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5417..5433)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5417..5433)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5430..5452)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5441..5457
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5465..5471,5472..5489,5490..5495))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5510..5531
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5648..5665)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5819..6407)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5899..5918)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6578..7369,7370..7438))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7201..7220
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7439..7543)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
61 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
301 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
361 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
421 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
481 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
541 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
601 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
661 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
721 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
781 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
841 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
901 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
961 cactataggg cgaattgggc ccaatgcatt ggcgccgcgg ccgcgaattc gagctcggta
1021 cccggggatc tggtgaccag ataagataat aacgacatga gaccaaagtt caactgacac
1081 gaattaaccg gtttattgat taacagacaa ttacagggga cgggtgaggt aacgggtgcc
1141 aatggggcga ggctcagtat aaagtccatt gttgtccaca gtgaaatcaa cgttggcaga
1201 ttttgcatag ttagatgtat actgcacttc gggattccag cgtttgctgt tttctttctg
1261 cagctcccat tcaatctcca cgctcacttg tcctgtggaa tactgggtga tgaatgaagc
1321 aaactttgta gccgaaaact ctgccggagg attcgcagga acaggcgtgt ttttgatgag
1381 gatctgagga ggcgggtgct taagtccaaa gccgcccatg agaggagacg ggtgaaagtg
1441 tccatccgtg tgaggaattt tggcccaaat aggaccctgc aggtatacgt ctctgtcttg
1501 ccacaccatt ccaggtaagg ctcccataac atgcacatct ccggtcgcag ggtctgtgct
1561 gctgctctgg agattgactg ccacagtccc aaatctttcg gtggccacgg ggttagtggc
1621 tttgatttcc tcttcgtctg tgatcatgac attgtccaat gcagtgtttg aagctccggc
1681 gctctccttt ccaaaaatca tgacaccgct catgggaaag aacttgtctt tgtcgtcttt
1741 gtgtgaggcc atagcagtgc cagggttgat tatagattca cgcccattaa ggttatattt
1801 tgaagcacca gtccaggtaa agttgctgtt gttgttgtct gtttttgttt tagaaacgcg
1861 ctgctgccgg taacagggtc caggtagcca gtttttgggc tgaacagaca tgccagctgg
1921 agacccccgg ctaaacagca agtccttgtt ttgggcactt ccggactgat tctgagttct
1981 gttcaggtaa tacaggtact ggtcgatgag aggattcatc agccggtcca ggctctggct
2041 gtgcgcgtag ctgctgtgga aaggcacgtc ctcgaaggtg tagctgaagg taaagttatt
2101 gcccgttctc agcatctgcg atgggaaata ttccaggcag taaaaggatg accgtcccac
2161 tgcctggctg ccattgttga gcgttaggta gccgtactgc ggaatcatga acacgtccgc
2221 cgggaacgga gggaggcagc cctggtgcgc agagccgagg acgtacggca actggtactc
2281 cgagtccgag aagacttgaa ccgtgctggt aaggttatta gcgatggtcg tgacgccatc
2341 attcgtcgtg acctccttga cttggatgtt gaagagcttg aagttgagtc tcttgggccg
2401 gaatccccaa ttgttgttga tgagtcgctg ccagtcacgt ggtgagaaat ggcagtggaa
2461 tctgttgaaa tcaaaatacc cccagggggt gctgtagccg aagtagtggt tgtcgttgct
2521 ggcccccgtt gaagcactgg agatttgctt gtagaggtgg ttgttatagg tgggcaaggc
2581 ccatgttcgg gtgctggtgg tgatgactct gtcgcccagc catgtggaat cgcaatgcca
2641 atttcctgag gcattaccca ctccgtcggc gccttcgtta ttgtctgcca ttggtgcgcc
2701 accgcctgaa gccattgtag taggtcccac agcagcgggg gttgctggag gttctccgag
2761 aggttgtggg tcggggactg actctgagtc gccagtctga ccaaaattga gtctcttttt
2821 agcgggctgc tggcctgtct tgccaatgcc cgaggaggag tctggctctt gtggcgactg
2881 ctctaccgga cgtttctttc caggagccgt cttagcacct tcctcaacca gaccaaaagg
2941 ttcgagaacc ctcttcttgg cctggaagac tgctcgcccg aggttgcccc caaaagacgt
3001 atcttcttgc agacgctcct gaaactcggc gtcggcgtgg ttataccgca ggtacggatt
3061 gtcacccgct ttgagctgct ggtcgtaggc cttgtcgtgc tcgagggccg ctgcatccgc
3121 cgcgttgacg ggctccccct tgtcgagtcc gttgaagggt ccgaggtact tgtagccagg
3181 aagcaccaga ccccggccgt cgtcctgctt ttgctggttg gctttgggtt tcggggctcc
3241 aggtttcaag tcccaccact cgcgaatgcc ctcagagagg ttgtcctcga gccaatctgg
3301 aagataacca tcggcagcca tacctggttt aagtcattta ttgctcagaa acacagtcat
3361 ccagatccac gttgaccaga tcgcaggccg agcaagcaat ctcgggagcc cgccccagca
3421 gatgatgaat ggcacagagt ttccgatacg tcctctttct gacgaccggt tgagattctg
3481 acacgccggg gaaacattct gaacagtctc tggtcccgtg cgtgaagcaa atgttgaaat
3541 tctgattcat tctctcgcat gttttgcagg gaaacagcat ctgaagcatg cccgcgtgac
3601 gagaacattt gttttggtac ctgtcggcaa agtccaccgg agctccttcc gcgtctgacg
3661 tcgatggatc cgcgactgag gggcaggccc gcttgggctc gcttttatcc gcgtcatcgg
3721 gggcgggtct cttgttggct ccaccctttc tgacgtagaa ctcatgcgcc acctcggtca
3781 cgtgatcctg cgcccagcgg aagaactctt tgacttcctg ctttgtcacc ttgccaaagt
3841 catgctccag acggcgggtg agttcaaatt tgaacatccg gtcctgcaac ggctgctggt
3901 gctcgaaggt ggtgctgttc ccgtcaatca cggcgcacat gttggtgttg gaggtgacga
3961 tcacgggggt gggatcgatc tgggcggacg acttgcactt ttggtccacg cgcaccttgc
4021 tgccgccgag aatggccttg gcggactcca cgaccttggc cgtcatcttg ccctcctccc
4081 accagatcac catcttgtcg acgcaatcgt tgaagggaaa gttctcattg gtccagttga
4141 cgcagccgta gaagggcacg gcgtgggcga tggcttccgc gatgttggtc ttgcccgtgg
4201 tggccggccc aaacagccag atggtgttgc gttttccgaa ccttttctgg gcccagccga
4261 gaaagacgga gccggcgtag gcagggtcgt agccgttcag ctccaggatg cggtaaatgc
4321 ggttggtttt aatgtcggcg ggcggagcgg ggcctaccag gtagtcgggc gcggatttgg
4381 tcagcgccat gatcttgccg gcattgtcca gagcggcctt gatctgggac cgcgagttgg
4441 aggcggcgtt gaaggagatg tacgaggcct ggtcctcctg gatccactgc ttctcggagg
4501 tgatgccccg gtccaccagc cacccgacca gctccatgta gcgtgcggag gtttttgacc
4561 ggatgacagg cgcgtcagaa ttggggttca gattctcctt gttctgctcc tgggtctggc
4621 tgacgtgggt caggtgctgc gccacgagcc gtttgcgctc ggccaggttt aaacacgcgc
4681 ttatatactc ctccatgtta gtccacgccc actgcagctc gggctgagtc ttgggcagga
4741 ggtagttggg gatgtagcac tcgtccacca ccttgttccc ccctccggcg ccattacgcg
4801 tcttggtcac cgcgaaccag ttgggcaggg tcggctcgat cccgcggtag atggtctgca
4861 ccagcttgtc cctaatctga ctcaggaagc ggcccagcac catggatttg acccccgtgg
4921 tctccaccag aatatggagg tggaagtagg actcgccctt ctcgaactga acaaagaaga
4981 gggcctccgg ggccttactc acgcggcgcc actggaccag gaagtcgcgc tgcagcttct
5041 cggccacggt caggggtgcc tgctcaatca gattcagatc catgtcagaa tctggcggca
5101 actcccattc cttctcggcc acccagttca caaagctgtc agaaatgccg ggcagatgct
5161 cgtcaaggtc gctggggacc ttaatcacaa tctcgtaaaa ccccggcatg gcggctgcgc
5221 gttcaaacct cccgcttcaa aatggagacc ctgcgtgctc actcgggctt aaatacccag
5281 cgtgaccaca tggtgtcgca aaatgtcgca aaacactcac gtgacctcta atacaagatc
5341 ctctagagtc gacctgcagg catgcaagct tttgttccct ttagtgaggg ttaatttcga
5401 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
5461 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
5521 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
5581 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
5641 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
5701 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
5761 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
5821 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
5881 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
5941 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6001 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6061 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6121 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6181 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6241 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
6301 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
6361 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6421 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
6481 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
6541 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
6601 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
6661 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
6721 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
6781 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
6841 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
6901 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
6961 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
7021 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
7081 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
7141 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
7201 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
7261 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
7321 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
7381 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
7441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
7501 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcg
//