LOCUS       pSpCas9(BB)-2A-GFP (PX458) Sequencing Result	9288 bp 	DNA	SYN
DEFINITION  pSpCas9(BB)-2A-GFP (PX458) Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="pSpCas9(BB)-2A-GFP (PX458) Sequencing Result"
                     /mol_type="other DNA"
     promoter        10..242
                     /label="hU6_promoter"
     misc_feature    172..191
                     /label="LKO1_5_primer"
     misc_feature    444..713
                     /label="CAG_enhancer"
     CDS             complement(1229..3886)
                     /label="ORF frame 3"
     CDS             1250..6310
                     /label="ORF frame 2"
     misc_feature    1253..1273
                     /label="FLAG"
     misc_feature    1274..1294
                     /label="FLAG"
     misc_feature    1295..1318
                     /label="FLAG"
     misc_feature    1325..1345
                     /label="SV40 NLS"
     misc_feature    1325..1348
                     /label="SV40 NLS"
     misc_feature    1331..1345
                     /label="NLS"
     gene            5588..6301
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(5630..5651)
                     /label="EGFP_N_primer"
     misc_feature    5768..5797
                     /label="Y66 (EGFP)"
     misc_feature    6238..6259
                     /label="EGFP_C_primer"
     misc_feature    complement(6329..6346)
                     /label="BGH_rev_primer"
     terminator      6332..6535
                     /label="bGH_PA_terminator"
     rep_origin      6783..7089
                     /label="f1_origin"
     misc_feature    7357..7379
                     /label="pGEX_3_primer"
     promoter        7538..7566
                     /label="AmpR_promoter"
     gene            7608..8468
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             7608..8468
                     /label="ORF frame 3"
     rep_origin      8623..9242
                     /label="pBR322_origin"
ORIGIN
    1 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG
   61 ATAATTGGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA
  121 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT
  181 ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
  241 CGAAACACCG GGTCTTCGAG AAGACCTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG
  301 GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT
  361 AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC
  421 AAATGGCTCT AGAGGTACCC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
  481 CCAACGACCC CCGCCCATTG ACGTCAATAG TAACGCCAAT AGGGACTTTC CATTGACGTC
  541 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC
  601 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TGTGCCCAGT
  661 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA
  721 CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC
  781 CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG
  841 GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA
  901 GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC
  961 GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG GGCGGGAGTC GCTGCGCGCT
 1021 GCCTTCGCCC CGTGCCCCGC TCCGCCGCCG CCTCGCGCCG CCCGCCCCGG CTCTGACTGA
 1081 CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC TCCTCCGGGC TGTAATTAGC
 1141 TGAGCAAGAG GTAAGGGTTT AAGGGATGGT TGGTTGGTGG GGTATTAATG TTTAATTACC
 1201 TGGAGCACCT GCCTGAAATC ACTTTTTTTC AGGTTGGACC GGTGCCACCA TGGACTATAA
 1261 GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT
 1321 GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA
 1381 CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA
 1441 CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA
 1501 GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA
 1561 GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT
 1621 CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT
 1681 CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA
 1741 GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG
 1801 CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG
 1861 GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT
 1921 CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG
 1981 CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT
 2041 GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG
 2101 CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA
 2161 GCTGAGCAAG GACACCTACG ACGACGACCT GGACAACCTG CTGGCCCAGA TCGGCGACCA
 2221 GTACGCCGAC CTGTTTCTGG CCGCCAAGAA CCTGTCCGAC GCCATCCTGC TGAGCGACAT
 2281 CCTGAGAGTG AACACCGAGA TCACCAAGGC CCCCCTGAGC GCCTCTATGA TCAAGAGATA
 2341 CGACGAGCAC CACCAGGACC TGACCCTGCT GAAAGCTCTC GTGCGGCAGC AGCTGCCTGA
 2401 GAAGTACAAA GAGATTTTCT TCGACCAGAG CAAGAACGGC TACGCCGGCT ACATTGACGG
 2461 CGGAGCCAGC CAGGAAGAGT TCTACAAGTT CATCAAGCCC ATCCTGGAAA AGATGGACGG
 2521 CACCGAGGAA CTGCTCGTGA AGCTGAACAG AGAGGACCTG CTGCGGAAGC AGCGGACCTT
 2581 CGACAACGGC AGCATCCCCC ACCAGATCCA CCTGGGAGAG CTGCACGCCA TTCTGCGGCG
 2641 GCAGGAAGAT TTTTACCCAT TCCTGAAGGA CAACCGGGAA AAGATCGAGA AGATCCTGAC
 2701 CTTCCGCATC CCCTACTACG TGGGCCCTCT GGCCAGGGGA AACAGCAGAT TCGCCTGGAT
 2761 GACCAGAAAG AGCGAGGAAA CCATCACCCC CTGGAACTTC GAGGAAGTGG TGGACAAGGG
 2821 CGCTTCCGCC CAGAGCTTCA TCGAGCGGAT GACCAACTTC GATAAGAACC TGCCCAACGA
 2881 GAAGGTGCTG CCCAAGCACA GCCTGCTGTA CGAGTACTTC ACCGTGTATA ACGAGCTGAC
 2941 CAAAGTGAAA TACGTGACCG AGGGAATGAG AAAGCCCGCC TTCCTGAGCG GCGAGCAGAA
 3001 AAAGGCCATC GTGGACCTGC TGTTCAAGAC CAACCGGAAA GTGACCGTGA AGCAGCTGAA
 3061 AGAGGACTAC TTCAAGAAAA TCGAGTGCTT CGACTCCGTG GAAATCTCCG GCGTGGAAGA
 3121 TCGGTTCAAC GCCTCCCTGG GCACATACCA CGATCTGCTG AAAATTATCA AGGACAAGGA
 3181 CTTCCTGGAC AATGAGGAAA ACGAGGACAT TCTGGAAGAT ATCGTGCTGA CCCTGACACT
 3241 GTTTGAGGAC AGAGAGATGA TCGAGGAACG GCTGAAAACC TATGCCCACC TGTTCGACGA
 3301 CAAAGTGATG AAGCAGCTGA AGCGGCGGAG ATACACCGGC TGGGGCAGGC TGAGCCGGAA
 3361 GCTGATCAAC GGCATCCGGG ACAAGCAGTC CGGCAAGACA ATCCTGGATT TCCTGAAGTC
 3421 CGACGGCTTC GCCAACAGAA ACTTCATGCA GCTGATCCAC GACGACAGCC TGACCTTTAA
 3481 AGAGGACATC CAGAAAGCCC AGGTGTCCGG CCAGGGCGAT AGCCTGCACG AGCACATTGC
 3541 CAATCTGGCC GGCAGCCCCG CCATTAAGAA GGGCATCCTG CAGACAGTGA AGGTGGTGGA
 3601 CGAGCTCGTG AAAGTGATGG GCCGGCACAA GCCCGAGAAC ATCGTGATCG AAATGGCCAG
 3661 AGAGAACCAG ACCACCCAGA AGGGACAGAA GAACAGCCGC GAGAGAATGA AGCGGATCGA
 3721 AGAGGGCATC AAAGAGCTGG GCAGCCAGAT CCTGAAAGAA CACCCCGTGG AAAACACCCA
 3781 GCTGCAGAAC GAGAAGCTGT ACCTGTACTA CCTGCAGAAT GGGCGGGATA TGTACGTGGA
 3841 CCAGGAACTG GACATCAACC GGCTGTCCGA CTACGATGTG GACCATATCG TGCCTCAGAG
 3901 CTTTCTGAAG GACGACTCCA TCGACAACAA GGTGCTGACC AGAAGCGACA AGAACCGGGG
 3961 CAAGAGCGAC AACGTGCCCT CCGAAGAGGT CGTGAAGAAG ATGAAGAACT ACTGGCGGCA
 4021 GCTGCTGAAC GCCAAGCTGA TTACCCAGAG AAAGTTCGAC AATCTGACCA AGGCCGAGAG
 4081 AGGCGGCCTG AGCGAACTGG ATAAGGCCGG CTTCATCAAG AGACAGCTGG TGGAAACCCG
 4141 GCAGATCACA AAGCACGTGG CACAGATCCT GGACTCCCGG ATGAACACTA AGTACGACGA
 4201 GAATGACAAG CTGATCCGGG AAGTGAAAGT GATCACCCTG AAGTCCAAGC TGGTGTCCGA
 4261 TTTCCGGAAG GATTTCCAGT TTTACAAAGT GCGCGAGATC AACAACTACC ACCACGCCCA
 4321 CGACGCCTAC CTGAACGCCG TCGTGGGAAC CGCCCTGATC AAAAAGTACC CTAAGCTGGA
 4381 AAGCGAGTTC GTGTACGGCG ACTACAAGGT GTACGACGTG CGGAAGATGA TCGCCAAGAG
 4441 CGAGCAGGAA ATCGGCAAGG CTACCGCCAA GTACTTCTTC TACAGCAACA TCATGAACTT
 4501 TTTCAAGACC GAGATTACCC TGGCCAACGG CGAGATCCGG AAGCGGCCTC TGATCGAGAC
 4561 AAACGGCGAA ACCGGGGAGA TCGTGTGGGA TAAGGGCCGG GATTTTGCCA CCGTGCGGAA
 4621 AGTGCTGAGC ATGCCCCAAG TGAATATCGT GAAAAAGACC GAGGTGCAGA CAGGCGGCTT
 4681 CAGCAAAGAG TCTATCCTGC CCAAGAGGAA CAGCGATAAG CTGATCGCCA GAAAGAAGGA
 4741 CTGGGACCCT AAGAAGTACG GCGGCTTCGA CAGCCCCACC GTGGCCTATT CTGTGCTGGT
 4801 GGTGGCCAAA GTGGAAAAGG GCAAGTCCAA GAAACTGAAG AGTGTGAAAG AGCTGCTGGG
 4861 GATCACCATC ATGGAAAGAA GCAGCTTCGA GAAGAATCCC ATCGACTTTC TGGAAGCCAA
 4921 GGGCTACAAA GAAGTGAAAA AGGACCTGAT CATCAAGCTG CCTAAGTACT CCCTGTTCGA
 4981 GCTGGAAAAC GGCCGGAAGA GAATGCTGGC CTCTGCCGGC GAACTGCAGA AGGGAAACGA
 5041 ACTGGCCCTG CCCTCCAAAT ATGTGAACTT CCTGTACCTG GCCAGCCACT ATGAGAAGCT
 5101 GAAGGGCTCC CCCGAGGATA ATGAGCAGAA ACAGCTGTTT GTGGAACAGC ACAAGCACTA
 5161 CCTGGACGAG ATCATCGAGC AGATCAGCGA GTTCTCCAAG AGAGTGATCC TGGCCGACGC
 5221 TAATCTGGAC AAAGTGCTGT CCGCCTACAA CAAGCACCGG GATAAGCCCA TCAGAGAGCA
 5281 GGCCGAGAAT ATCATCCACC TGTTTACCCT GACCAATCTG GGAGCCCCTG CCGCCTTCAA
 5341 GTACTTTGAC ACCACCATCG ACCGGAAGAG GTACACCAGC ACCAAAGAGG TGCTGGACGC
 5401 CACCCTGATC CACCAGAGCA TCACCGGCCT GTACGAGACA CGGATCGACC TGTCTCAGCT
 5461 GGGAGGCGAC AAAAGGCCGG CGGCCACGAA AAAGGCCGGC CAGGCAAAAA AGAAAAAGGA
 5521 ATTCGGCAGT GGAGAGGGCA GAGGAAGTCT GCTAACATGC GGTGACGTCG AGGAGAATCC
 5581 TGGCCCAGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
 5641 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
 5701 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
 5761 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
 5821 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
 5881 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
 5941 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
 6001 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
 6061 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
 6121 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
 6181 CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
 6241 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
 6301 GGAATTCTAA CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT
 6361 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT
 6421 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG
 6481 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG AGAATAGCAG GCATGCTGGG
 6541 GAGCGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG
 6601 CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA
 6661 GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA TGCGGTATTT TCTCCTTACG
 6721 CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC ATAGTACGCG CCCTGTAGCG
 6781 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG
 6841 CCTTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC
 6901 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
 6961 TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA
 7021 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA
 7081 CTGGAACAAC ACTCAACTCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA
 7141 TTTCGGTCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA
 7201 AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT
 7261 AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
 7321 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTccgg gagctgcatg tgtcagaggt
 7381 tttcaccgtc atcaccgaaa cgcgcgagAC GAAAGGGCCT CGTGATACGC CTATTTTTAT
 7441 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG
 7501 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
 7561 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
 7621 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
 7681 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
 7741 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
 7801 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG
 7861 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
 7921 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
 7981 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
 8041 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
 8101 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
 8161 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
 8221 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
 8281 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGAAGCCGC GGTATCATTG
 8341 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
 8401 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
 8461 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
 8521 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
 8581 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
 8641 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
 8701 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
 8761 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
 8821 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
 8881 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
 8941 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
 9001 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
 9061 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
 9121 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
 9181 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
 9241 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGT
//