| 質粒名稱: | pTarget_CAST |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 127926 |
| 存儲實驗室: | Feng Zhang |
|
載體骨架基本信息 |
|
| 載體名稱: | pACYC |
| 載體出品公司: | -- |
| 空載體大小: | -- |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | -- |
| 篩選標記: | -- |
| 載體抗性: | 氯霉素,25 μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 低拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | PSP1 target (GGTT PAM) |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子: | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位點: | -- |
| 3’克隆位點: | -- |
| 5’測序引物 | -- |
| 3’測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pTarget_CAST was a gift from Feng Zhang (Addgene plasmid # 127926 ; //n2t.net/addgene:127926 ; RRID:Addgene_127926) |
| 參考文獻部分: | RNA-guided DNA insertion with CRISPR-associated transposases. Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F. Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. Epub 2019 Jun 6. 10.1126/science.aax9181 PubMed 31171706 |
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pTarget_CAST載體 pTargetCAST質粒基本信息
pTarget_CAST質粒圖譜 pTargetCAST載體圖譜和pTarget_CAST載體序列 pTargetCAST質粒序列多克隆位點信息
pTarget_CAST質粒 pTargetCAST載體簡介
pTarget_CAST質粒序列 pTargetCAST載體序列
LOCUS pTarget_CAST 2899 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Target plasmid containing PSP1.
ACCESSION .
VERSION .
KEYWORDS pTarget_CAST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2899)
AUTHORS Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin
EV, Zhang F
TITLE RNA-guided DNA insertion with CRISPR-associated transposases.
JOURNAL Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181.
Epub 2019 Jun 6.
PUBMED 31171706
REFERENCE 2 (bases 1 to 2899)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..2899
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(288..305)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(422..967)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(502..521)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(1583..1602)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(2033..2692)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 2607..2626
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(2693..2795)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
ORIGIN
1 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
61 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
121 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
181 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
241 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
301 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
361 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
421 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
601 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
661 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
721 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
781 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
841 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
901 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
961 atctcaagaa gatcatctta ttaatatacg tagtatctac gatacgtagt atctacgata
1021 cgtaggaggt tgagaagtca tttaataagg ccactgttaa acgtagatat atctacgcgt
1081 agatatatct acgatacgta gcggccgacg cgctgggcta cgtcttaatg ggcagcgccc
1141 acatacgcag cgatttccaa cagcttgagg cggaagcttt aatgcggtag tttatcacag
1201 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
1261 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
1321 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
1381 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
1441 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
1501 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
1561 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggcc
1621 tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc
1681 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctaaccgtt
1741 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
1801 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
1861 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
1921 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
1981 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
2041 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
2101 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
2161 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
2221 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
2281 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
2341 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
2401 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
2461 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
2521 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
2581 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
2641 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
2701 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
2761 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
2821 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
2881 ccgtcacagg tatttattc
//
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