LOCUS pUCmini-iCAP-AAV.CAP-B10 8543 bp ds-DNA circular SYN 05-NOV-2021
DEFINITION non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap
expression controlled by a tTA-TRE amplifcation system.
ACCESSION .
VERSION .
KEYWORDS pUCmini-iCAP-AAV.CAP-B10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8543)
TITLE Brain-wide gene expression with decreased liver targeting after
intravenous delivery of engineered AAV capsid in mouse and marmoset
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8543)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 5, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..8543
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 130..152
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 144..161
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 145..161
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 171..190
/label=T7
/note="T7 promoter, forward primer"
promoter 171..189
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2779..2889
/codon_start=1
/product="3 tandem repeats of the minimal activation domain
of herpes simplex virus VP16 (Cheng et al., 2013)"
/label=VP48
/translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
promoter complement(2928..2946)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2929..2946)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2966..2983)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2972..3196
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 3258..3276
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3295..3313
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3332..3350
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3369..3387
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3406..3424
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3443..3461
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3480..3498
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3517..3535
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 6252..6263
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
rep_origin complement(6819..7407)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6899..6918)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7578..8438)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8201..8220
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8439..8543)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cacatttccc cgaaaagtgc cacctgacgt cccaatgata cgcgtcggtg cgggcctctt
61 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
121 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
181 cactataggg cgaattgggt acatcgacgg tatcggggga gctcgcaggg tctccatttt
241 gaagcgggag gtttgaacgc gcagccgcca tgccggggtt ttacgagatt gtgattaagg
301 tccccagcga ccttgacgag catctgcccg gcatttctga cagctttgtg aactgggtgg
361 ccgagaagga atgggagttg ccgccagatt ctgacatgga tctgaatctg attgagcagg
421 cacccctgac cgtggccgag aagctgcagc gcgactttct gacggaatgg cgccgtgtga
481 gtaaggcccc ggaggctctt ttctttgtgc aatttgagaa gggagagagc tacttccaca
541 tgcacgtgct cgtggaaacc accggggtga aatccatggt tttgggacgt ttcctgagtc
601 agattcgcga aaaactgatt cagagaattt accgcgggat cgagccgact ttgccaaact
661 ggttcgcggt cacaaagacc agaaatggcg ccggaggcgg gaacaaggtg gtggatgagt
721 gctacatccc caattacttg ctccccaaaa cccagcctga gctccagtgg gcgtggacta
781 atatggaaca gtatttaagc gcctgtttga atctcacgga gcgtaaacgg ttggtggcgc
841 agcatctgac gcacgtgtcg cagacgcagg agcagaacaa agagaatcag aatcccaatt
901 ctgatgcgcc ggtgatcaga tcaaaaactt cagccaggta catggagctg gtcgggtggc
961 tcgtggacaa ggggattacc tcggagaagc agtggatcca ggaggaccag gcctcataca
1021 tctccttcaa tgcggcctcc aactcgcggt cccaaatcaa ggctgccttg gacaatgcgg
1081 gaaagattat gagcctgact aaaaccgccc ccgactacct ggtgggccag cagcccgtgg
1141 aggacatttc cagcaatcgg atttataaaa ttttggaact aaacgggtac gatccccaat
1201 atgcggcttc cgtctttctg ggatgggcca cgaaaaagtt cggcaagagg aacaccatct
1261 ggctgtttgg gcctgcaact accgggaaga ccaacatcgc ggaggccata gcccacactg
1321 tgcccttcta cgggtgcgta aactggacca atgagaactt tcccttcaac gactgtgtgg
1381 acaagatggt gatctggtgg gaggagggga agatgaccgc caaggtcgtg gagtcggcca
1441 aagccattct cggaggaagc aaggtgcgcg tggaccagaa atgcaagtcc tcggcccaga
1501 tagacccgac tcccgtgatc gtcacctcca acaccaatat gtgcgccgtg attgacggga
1561 actcaacgac cttcgaacac cagcagccgt tgcaagaccg gatgttcaaa tttgaactca
1621 cccgccgtct ggatcatgac tttgggaagg tcaccaagca ggaagtcaaa gactttttcc
1681 ggtgggcaaa ggatcacgtg gttgaggtgg agcatgaatt ctacgtcaaa aagggtggag
1741 ccaagaaaag acccgccccc agtgacgcag atataagtga gcccaaacgg gtgcgcgagt
1801 cagttgcgca gccatcgacg tcagacgcgg aagcttcgat caactacgcg gacaggtacc
1861 aaaacaaatg ttctcgtcac gtgggcatga atctgatgct gtttccctgt agacaatgcg
1921 agagactgaa tcagaattca aatatctgct tcactcacgg tgtcaaagac tgtttagagt
1981 gctttcccgt gtcagaatct caacccgttt ctgtcgtcaa aaaggcgtat cagaaactgt
2041 gctacattca tcacatcatg ggaaaggtgc cagacgcttg cactgcttgc gacctggtca
2101 atgtggactt ggatgactgt gtttctgaac aataaatgac ttaaaccagg tatgagtcgg
2161 ctggataaat ctaaagtcat aaacggcgct ctggaattac tcaatgaagt cggtatcgaa
2221 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
2281 cacgtgaaga acaagcgggc cctgctcgat gccctggcca tcgagatgct ggacaggcat
2341 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
2401 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
2461 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
2521 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
2581 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
2641 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
2701 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
2761 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
2821 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
2881 gacatgctcc ccgggtaaat gcatgaattc gatctagagg gccctattct atagtgtcac
2941 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
3001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
3061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
3121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
3181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgaa tcaagctatc
3241 aagtgccacc tgacgtctcc ctatcagtga tagagaagtc gacacgtctc gagctcccta
3301 tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga agtcgacacg
3361 tctcgagctc cctatcagtg atagagaagg tacgtctaga acgtctccct atcagtgata
3421 gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc tagaacgtct
3481 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
3541 ccccctatat aagcagagag atctgttcaa atttgaactg actaagcggc tcccgccaga
3601 ttttggcaag attactaagc aggaagtcaa ggactttttt gcttgggcaa aggtcaatca
3661 ggtgccggtg actcacgagt ttaaagttcc cagggaattg gcgggaacta aaggggcgga
3721 gaaatctcta aaacgcccac tgggtgacgt caccaatact agctataaaa gtctggagaa
3781 gcgggccagg ctctcatttg ttcccgagac gcctcgcagt tcagacgtga ctgttgatcc
3841 cgctcctctg cgaccgctag cttcgatcaa ctacgcagac aggtaccaaa acaagtgttc
3901 tcgtcacgtg ggcattaatc tgattctgtt tccctgcaga caatgcgaga gaatgaatca
3961 gaactcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc
4021 agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca
4081 tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg tggatttgga
4141 tgactgcatc tttgaacaat aaatgactta agccaggtat ggctgccgat ggttatcttc
4201 cagattggct cgaggacaac cttagtgaag gaattcgcga gtggtgggct ttgaaacctg
4261 gagcccctca acccaaggca aatcaacaac atcaagacaa cgctagaggt cttgtgcttc
4321 cgggttacaa ataccttgga cccggcaacg gactcgacaa gggggagccg gtcaacgcag
4381 cagacgcggc ggccctcgag cacgacaaag cctacgacca gcagctcaag gccggagaca
4441 acccgtacct caagtacaac cacgccgacg ccgagttcca ggagcggctc aaagaagata
4501 cgtcttttgg gggcaacctc gggcgagcag tcttccaggc caaaaagagg cttcttgaac
4561 ctcttggtct ggttgaggaa gcggctaaga cggctcctgg aaagaagagg cctgtagagc
4621 agtctcctca ggaaccggac tcctccgcgg gtattggcaa atcgggtgca cagcccgcta
4681 aaaagagact caatttcggt cagactggcg acacagagtc agtcccagac cctcaaccaa
4741 tcggagaacc tcccgcagcc ccctcaggtg tgggatctct tacaatggct tcaggtggtg
4801 gcgcaccagt ggcagacaat aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt
4861 ggcattgcga ttcccaatgg ctgggggaca gagtcatcac caccagcacc cgaacctggg
4921 ccctgcccac ctacaacaat cacctctaca agcaaatctc caacagcaca tctggaggat
4981 cttcaaatga caacgcctac ttcggctaca gcaccccctg ggggtatttt gacttcaaca
5041 gattccactg ccacttctca ccacgtgact ggcagcgact catcaacaac aactggggat
5101 tccggcctaa gcgactcaac ttcaagctct ttaacattca ggtcaaagag gttacggaca
5161 acaatggagt caagaccatc gccaataacc ttaccagcac ggtccaggtc ttcacggact
5221 cagactatca gctcccgtac gtgctcgggt cggctcacga gggctgcctc ccgccgttcc
5281 cagcggacgt tttcatgatt cctcagtacg ggtatctgac gcttaatgat ggaagccagg
5341 ccgtgggtcg ttcgtccttt tactgcctgg aatatttccc gtcgcaaatg ctaagaacgg
5401 gtaacaactt ccagttcagc tacgagtttg agaacgtacc tttccatagc agctacgctc
5461 acagccaaag cctggaccga ctaatgaatc cactcatcga ccaatacttg tactatctct
5521 ctagaactat tgacggcgcc gccaccaaaa accaaacgct aaaattcagt gtggccggac
5581 ccagcaacat ggctgtccag ggaagaaact acatacctgg acccagctac cgacaacaac
5641 gtgtctcaac cactgtgact caaaacaaca acagcgaatt tgcttggcct ggagcttctt
5701 cttgggctct caatggacgt aatagcttga tgaatcctgg acctgctatg gcctctcaca
5761 aagaaggaga ggaccgtttc tttcctttgt ctggatcttt aatttttggc aaacaaggta
5821 ctggcagaga caacgtggat gcggacaaag tcatgataac caacgaagaa gaaattaaaa
5881 ctactaaccc ggtagcaacg gagtcctatg gacaagtggc cacaaaccac cagagtgatg
5941 ggactttggc ggtgcctttt aaggcacagg cgcagaccgg ttgggttcaa aaccaaggaa
6001 tacttccggg tatggtttgg caggacagag atgtgtacct gcaaggaccc atttgggcca
6061 aaattcctca cacggacggc aactttcacc cttctccgct gatgggaggg tttggaatga
6121 agcacccgcc tcctcagatc ctcatcaaaa acacacctgt acctgcggat cctccaacgg
6181 ccttcaacaa ggacaagctg aactctttca tcacccagta ttctactggt caagtcagcg
6241 tggagatcga gtgggagctg cagaaggaaa acagcaagcg ctggaacccg gagatccagt
6301 acacttccaa ctattacaag tctaataatg ttgaatttgc tgttaatact gaaggtgtat
6361 atagtgaacc ccgccccatt ggcaccagat acctgactcg taatctgtaa gtcgacttgc
6421 ttgttaatca ataaaccgtt taattcgttt cagttgaact ttggtctctg cgaagggcaa
6481 ttcgtttaaa cctgcaggac tagaggtcct gtattagagg tcacgtgagt gttttgcgac
6541 attttgcgac accatgtggt cacgctgggt atttaagccc gagtgagcac gcagggtctc
6601 cattttgaag cgggaggttt gaacgcgcag ccgccaagcc gaattctgca gatatcacat
6661 gtcctaggaa ctatcgatcc atcacactgg cggccgctcg actagagcgg ccgccaccgc
6721 ggtggagctc cagcttttgc ggaccgaatc ggaaagaaca tgtgagcaaa aggccagcaa
6781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
6841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
6901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
6961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
7021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
7081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
7141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
7201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
7261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
7321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
7381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
7681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
8521 aataaacaaa taggggttcc gcg
//
