LOCUS       pYSEAP               5297 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pYSEAP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Pastrana DV, Buck CB, Pang YY, Thompson CD, Castle PE, FitzGerald 
            PC, Kruger Kjaer S, Lowy DR, Schiller JT
  TITLE     Reactivity of human sera in a sensitive, high-throughput 
            pseudovirus-based papillomavirus neutralization assay for HPV16 and 
            HPV18.
  JOURNAL   Virology. 2004 Apr 10;321(2):205-16.
  PUBMED    15051381
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        462..1640
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          693..1631
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1588..1608
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1727..3229
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    3287..3408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3298..3317)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3352..3371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3656..3673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3907..3926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4445..4840)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4859..4906)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4954..5284)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4968..5103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5022..5041)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5269..5289
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cgcgttgaca ttgattattg actagttagt tattactagc cgcgtgcaaa gcaccggcgg
       61 cggtagatgc ggggtaagta ctgaatttta attcgaccta tcccggtaaa gcgaaagcga
      121 cacgcttttt tttcacacat agcgggaccg aacacgttat aagtatcgaa ctcctaaaga
      181 agcagtgtag ttttctgcag atgcaaaaaa gatctcatga aggaggaact tgtgcagttt
      241 acttaatctg ctttaacaca gctaaaagca gagaaacagt ccggaatctg atggcaaaca
      301 cgctaaatgt aagagaagag tgtttgatgc tgcagccagc taaaattcga ggactcagcg
      361 cagctctatt ctggtttagc ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
      421 gcagctaatg gaccttgtag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
      481 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
      541 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      601 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      661 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      721 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      781 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      841 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      901 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      961 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     1021 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     1081 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     1141 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     1201 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     1261 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     1321 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     1381 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     1441 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1501 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1561 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1621 ttccatttca ggtgtcgtga ggaattctct agagatccct cgacctcgag atccattgtg
     1681 ctggatctgc gatctaagta agcttcgaat cgcgaattcg cccaccatgc tgctgctgct
     1741 gctgctgctg ggcctgaggc tacagctctc cctgggcatc atcccagttg aggaggagaa
     1801 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     1861 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     1921 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     1981 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     2041 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     2101 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     2161 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     2221 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     2281 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     2341 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     2401 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     2461 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     2521 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     2581 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     2641 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     2701 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     2761 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     2821 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     2881 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     2941 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     3001 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     3061 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     3121 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     3181 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     3241 gggttactct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     3301 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3361 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3421 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     3481 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     3541 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     3601 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     3661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4261 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4441 ggtcgccctc ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct
     4501 gtccatcact gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc
     4561 ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag
     4621 tcaggttgcc agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc
     4681 ccccagtaaa atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg
     4741 cgctggcgac gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt
     4801 caatgagggt ggattcttct tgagacaaag gcttggccat ggtttagttc ctcaccttgt
     4861 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga
     4921 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
     4981 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat
     5041 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     5101 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     5161 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     5221 catgctttgc atacttctgc ctgcctgggg agcctgggga ctttccacac cctaactgac
     5281 acacattcca cagaatt
//