LOCUS       pcDNA5FRT/Flag-OGT1               8193 bp ds-DNA     circular SYN 07-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5FRT/Flag-OGT1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, 
            Conaway JW, Conaway RC
  TITLE     Subunit composition and substrate specificity of a MOF-containing 
            histone acetyltransferase distinct from the male-specific lethal 
            (MSL) complex.
  JOURNAL   J Biol Chem. 2010 Feb 12. 285(7):4268-72.
  PUBMED    20018852
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 7, 2020 from SnapGene Server 1.1.58
            //www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(54..73)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        245..624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     779..799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     873..892
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        873..891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             917..940
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4155..4172)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4161..4385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4518..4537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4669..4716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4724..5746
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5876..5997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5913..5932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5967..5986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6046..6062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6046..6062)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6059..6081)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6070..6086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6094..6124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6139..6160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6277..6294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6448..7036)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6528..6547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7207..8067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7830..7849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8068..8172)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
       61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
      121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      841 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      901 gctggctagc atgccagact acaaggacga tgatgacaag ctcgacatgg cgtcttccgt
      961 gggcaacgtg gccgacagca cagggttagc tgagttggca catcgagaat atcaggcagg
     1021 agattttgag gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac
     1081 tggtgtgctt ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc
     1141 tcactttagc actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt
     1201 ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc
     1261 attgcgtctc aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc
     1321 agcgggtgac atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga
     1381 tttgtactgt gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga
     1441 agccaaggca tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag
     1501 taatcttggc tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga
     1561 aaaggctgtc acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt
     1621 gaaagaggca cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag
     1681 tccaaatcac gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat
     1741 agatctggca atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc
     1801 ttactgcaac ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg
     1861 ttataataca gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa
     1921 tatcaaacga gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga
     1981 agtcttccca gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg
     2041 aaaactgcag gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc
     2101 tgatgcctac tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt
     2161 gcagtgttat acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct
     2221 ggcttccatt cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc
     2281 tctgaaactt aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat
     2341 tgtctgtgat tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga
     2401 ccagttagag aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct
     2461 ttctcatggc ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat
     2521 taatgttctt cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg
     2581 gctgcgtgta ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca
     2641 gtctattcca ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc
     2701 agacgatggc acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct
     2761 ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat
     2821 ccttgtaaat atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc
     2881 agctcctatt caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga
     2941 ttatattatc actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa
     3001 attggcttat atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct
     3061 gaagaaaaaa gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt
     3121 tctgaatggc atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa
     3181 gatgaagtgt cctgatggag gagacgatgc agatagcagt aacacagctc ttaatatgcc
     3241 tgttattcct atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat
     3301 tcaaataaca attaatggat tcagtattag caatggactg gcaactactc agatcaacaa
     3361 taaggctgca actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta
     3421 cgggttacca gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc
     3481 ttctactttg cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct
     3541 gttgcgtttt ccagcagtag gagaacctaa tattcaacag tatgcacaga acatgggcct
     3601 gccccagaac cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg
     3661 ccagctggct gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga
     3721 tgtcctctgg gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt
     3781 tgcagcatcc cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga
     3841 atatgaagac atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg
     3901 caaagtctgg aagcaaagaa tatctagccc tctgttcaac accgaacaat acacaatgga
     3961 actagagcgg ctctatctac agatgtggga gcattatgca gccggcaaca aacctgacca
     4021 catgattaag cctgttgaag tcactgagtc agcataagga tccactagtc cagtgtggtg
     4081 gaattctgca gatatccagc acagtggcgg ccgctcgatc gagtctagag ggcccgttta
     4141 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4201 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4261 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4321 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4381 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4501 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4561 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4621 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
     4681 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
     4741 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     4801 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     4861 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     4921 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     4981 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     5041 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     5101 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     5161 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     5221 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     5281 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     5341 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     5401 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     5461 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     5521 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     5581 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     5641 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     5701 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
     5761 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5821 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6001 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8161 aggggttccg cgcacatttc cccgaaaagt gcc
//