| 質粒名稱: | xCas12i |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 195333 |
| 存儲實驗室: | Yingsi Zhou |
|
載體骨架基本信息 |
|
| 載體名稱: | px330 |
| 載體出品公司: | Feng Zhang (Addgene plasmid # 42230) |
| 完整質粒大小: | 9321bp |
| 載體類型: | 哺乳細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | human codon-optimized xCas12i |
| 物種來源: | Synthetic |
| 插入大小: | 951 bp |
| 啟動子: | CBh, CMV, hU6 |
| 標簽/融合蛋白: | 3xFlag (N terminal on insert) |
| 克隆方法: | Gibson Cloning |
|
文獻引用方法 |
|
| 材料和方法部分: | xCas12i was a gift from Yingsi Zhou (Addgene plasmid # 195333 ; //n2t.net/addgene:195333 ; RRID:Addgene_195333) |
| 參考文獻部分: | An engineered xCas12i with high activity, high specificity, and broad PAM range. Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, Li J, Zhong N, Zhang M, Zhou Y, Yang H. Protein Cell. 2023 Jun 28;14(7):538-543. doi: 10.1093/procel/pwac052. 10.1093/procel/pwac052 PubMed 37378659 |
買家導航
xCas12i載體質粒基本信息
xCas12i質粒圖譜載體圖譜和xCas12i載體序列質粒序列多克隆位點信息
xCas12i質粒載體簡介
xCas12i質粒序列載體序列
LOCUS xCas12i
9321 bp ds-DNA circular SYN 30-JAN-2023
DEFINITION vector for encoding a human codon-optimized xCas12i driven by CBh
promoter, guide RNAs compatible with xCas12i driven by hU6, and
mCherry driven by CMV promoter.
ACCESSION .
VERSION .
KEYWORDS xCas12i
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9321)
AUTHORS Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang
W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y,
Li J, Zhong N, Zhang M, Zhou Y, Yang H
TITLE An engineered xCas12i with high activity, high specificity and broad
PAM range
JOURNAL
REFERENCE 2 (bases 1 to 9321)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 30, 2023 from SnapGene Server 1.1.58
//gt12365.cn
FEATURES Location/Qualifiers
source 1..9321
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 299..374
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 471..756
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 758..1035
/label=chicken beta-actin promoter
intron 1036..1263
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1275..1284
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1284..1349
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1356..1376
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4641..4688
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(4716..4733)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4722..4929
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
enhancer 4998..5301
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5302..5505
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 5456..5476
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 5534..6244
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(5679..5697)
/label=mCherry-R
/note="mCherry, reverse primer"
rep_origin 6799..7254
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6886..6905)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7096..7117
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7271..7290)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7390..7412
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7450..7468)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7536..7640
/gene="bla"
/label=AmpR promoter
CDS 7641..8501
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7859..7878)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8672..9260
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9161..9180
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg agaaatgtgt ccccagttga cacagagacc gagaggtctc gtttttttgt
301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
361 caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc
421 cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa
481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
541 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
601 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
661 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
721 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
781 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
841 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
901 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
961 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
1021 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
1081 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
1141 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
1201 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
1261 caggttggac cggtgccacc atggactata aggaccacga cggagactac aaggatcatg
1321 atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta
1381 tccacggagt cccagcagcc atgagttctg atgtggtgcg gccttataac acaaagctgc
1441 tcccagataa cagaaagcac aatatgttcc tgcagacctt caagcggctg aacagcatct
1501 ctctgaacca cttcgacctg ctgatctgcc tgtacgctgc aatcaccaac aagaaggccg
1561 aggaatacaa gtctgaaaag gaagcccacg tgaccgccga tagcctgtgt gccatcaatt
1621 ggttcagacc catgagcaag agatacagca aatacgccac caccaccttc aacatgttag
1681 aactgtttaa ggagtacagc ggccacgagc ctgatgccta ttccaagaac tacctgatga
1741 gcaatatcga cagcgacaga ttcgtgtggg tggattgtag gaagttcgct aaggactttg
1801 cctatcagat ggaactgggt ttccacgagt tcaccgtgtt ggccgaaacc ctgctggcta
1861 attctatcct ggtgctgaac gagagcacca aggccaattg ggcttgggga accgtgtctg
1921 ccctgtacgg cggcggagat aaggaggaca gcacactgaa gagcaagatt ctgctggcct
1981 tcgtggacgc cctgaacaac cacgagctga aaacaaagag agaaatcttg aatcaagtgt
2041 gtgaatctct gaaataccag agctaccagg acatgtacgt ggattttaga agcgtggttg
2101 acgaaaacgg caacaagaag tctcctaacg gctctatgcc tatcgtgacc aagttcgaga
2161 cagacgacct gatcagcgac aaccaaagaa aggccatgat cagcaacttc actaagaacg
2221 ccgctgccaa ggcagctaag aaacctatcc cttacttgga ccgcctgaag gagcacatgg
2281 tgtccctgtg cgacgagtac aatgtgtatg cctgggccgc ggccatcaca aacagcaacg
2341 ccgacgtgac cgcccggaat accagaaacc tgacattcat cggcgaacag aacagcagac
2401 gaaaggaact gagcgtgctg cagacaacaa ccaacgagaa ggctaaggac atcctgaaca
2461 agatcaacga caacctgatt caggaggtgc ggtacacccc tgcccctaag cacctgggca
2521 gagatctggc caacctgttt gatacactga aggaaaagga catcaacaac atcgagaacg
2581 aagaagagaa acagaacgtg atcaatgact gtatcgagca gtacgtggac gattgcagaa
2641 gcctcaaccg gaaccccatc gcagccctcc tgaagcacat ctctaggtac tacgaggatt
2701 tcagcgccaa gaatttcctg gacggcgcca agctgaacgt gctgactgag gtggtgaacc
2761 ggcagaaggc ccaccccacc atctggagcg agaaggctta cacctggatc agcaagttcg
2821 acaagaaccg gagacaggcc aacagcagcc tggtcggatg ggttgtgccc cccgaggagg
2881 tgcacaagga gaaaatcgcc ggacagcaga gcatgatgtg ggtgaccctc accctgctgg
2941 acgacggcaa gtgggtcaaa catcacatcc ccttcagcga cagcagatac tacagcgaag
3001 tgtacgccta caaccctaat ctgccttatc tggacggagg catcccaaga cagagcaagt
3061 tcggcaacaa accaacaacc aacctgacag ccgagtccca ggccctcctg gctaattcta
3121 agtacaagaa agccaacaag agcttcctgc gggctaaaga gaatgccaca cacaacgtgc
3181 gggtgtcccc taacacctct ctgtgcatta gactgctgaa ggacagcgcc ggaaaccaga
3241 tgttcgacaa aatcggcaac gtgctcttcg gcatgcagat caaccacaag atcaccgtgg
3301 gaaaacctaa ctacaagatc gaggtgggcg acagattcct gggcttcgat cagaaccaga
3361 gcgagaacca cacctacgcc gtgctgcaga gagtgtccga gagcagtcac gacacccacc
3421 actttaacgg ctgggacgtg aaggtgctgg aaaagggcaa agtgaccagc gatgtgatcg
3481 tgcgggacga ggtctacgac caactgtctt acgagggcgt cccctacgat agcagcaagt
3541 tcgccgagtg gcgggacaag cgcagaagat ttgtgcttga gaacctgagc atccagctgg
3601 aagagggcaa gaccttcctg acagagttcg acaagctgaa taaggacagc ctgtaccgct
3661 ggaacatgaa ctacctgaaa ctgctgagaa aggccatccg ggccggaggc aaagagttcg
3721 ccaagatcgc taagacagag atcttcgagc tggcggtgga aagattcggc cctattaacc
3781 tgggcagcct gtcccagatc agccttaaga tgattgcctc ctttaagggc gtggtccagt
3841 cctacttctc cgtgagcggc tgcgtggatg atgcctccaa aaaggcccat gattctatgc
3901 tgttcacatt tatgtgcgcc gccgaagaaa agcggaccaa caagagagaa gaaaagacca
3961 acagagccgc cagctttatc ctgcaaaaag cctacctgca tggctgcaag atgatcgtgt
4021 gcgaggacga ccttcctgtg gccgacggca agacaggcaa agcccagaat gccgaccgga
4081 tggactggtg cgccagagcc ctggccaaga aggtgaacga cggctgtgtt gccatgagca
4141 tctgctacag agctatccct gcctacatga gcagccacca ggaccccttt gtgcacatgc
4201 aggataagaa aaccagcgtg ctgcggccta gattcatgga agttaataag gatagcatca
4261 gagactacca cgtggcgggc ctgagaagaa tgctgaacag caagagtgac gctggcacca
4321 gtgtttatta ccggcaagct gccctgcatt tctgcgaagc cctgggcgtg agccctgaac
4381 tggtgaaaaa caagaaaacc cacgccgccg aactgggcaa gcacatgggc agcgctatgc
4441 tgatgccctg gagaggcggt agagtgtaca tcgccagcaa aaagctgacc tccgatgcca
4501 aatcagtgaa gtactgcggc gaggatatgt ggcagtacca cgccgatgag atcgccgctg
4561 ttaacatcgc catgtatgag gtgtgctgcc agaccggcgc tttcggaaag aaacagaaaa
4621 aatcggacga gctgcctgga aaaaggccgg cggccacgaa gaaggccggc caggcaaaga
4681 agaagaagta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
4741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
4801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
4861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
4921 tgctggggag cggccgctag ttattaatag taatcaatta cggggtcatt agttcatagc
4981 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
5041 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
5101 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
5161 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
5221 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
5281 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
5341 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
5401 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
5461 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
5521 cagatccgct agcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
5581 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
5641 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
5701 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
5761 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
5821 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
5881 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
5941 cgacggcccc gtaatgcaga agaaaaccat gggctgggag gcctcctccg agcggatgta
6001 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
6061 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
6121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
6181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
6241 gtaaggatcc gggatgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat
6301 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
6361 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
6421 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
6481 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata
6541 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
6601 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
6661 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct ggtcgacctg
6721 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
6781 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
6841 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
6901 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
6961 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
7021 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
7081 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
7141 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
7201 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
7261 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
7321 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
7381 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
7441 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
7501 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
7561 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
7621 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
7681 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
7741 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
7801 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
7861 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
7921 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
7981 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
8041 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
8101 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
8161 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
8221 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
8281 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
8341 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
8401 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
8461 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
8521 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
8581 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
8641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
8701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
8761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
8821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
8881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
8941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
9001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
9061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
9121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
9181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
9241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
9301 gctggccttt tgctcacatg t
//