| 培養基名稱 | 改良Giolitti-Cantoni肉湯，Giolitti-Cantoni肉湯（ISO標準 ） |
|---|---|
| 英文名稱: |
Modified Giolitti and Cantoni Broth (ISO) |
| 培養基類型: | 選擇性培養基 |
| 產品目錄號: | M731-01 |
| 產品規格: |
250g 其它規格請聯系我們 |
| 保存條件: | 密封，陰涼干燥處保存。制備好的培養基2 - 8°C避光保存。 |
| 產品性狀: |
麥秸色均一粉末。 麥秸色透明溶液。 |
| 注意事項: | 避免攝入、呼入、皮膚接觸。配制時在通風櫥中進行，戴口罩 、 手套、護目鏡。 |
| 相關產品: | -- |
產品描述：
Giolitti-Cantoni肉湯基礎(Modified Giolitti-Cantoni Broth) 是選擇性液體培養基，是ISO 6888-3:2003規定的MPN法檢測和增菌食品和飼料中的凝固酶陽性葡萄球菌 用培養基。本配方是ISO標準配方。
Giolitti-Cantoni肉湯工作原理
牛肉提取物、酵母提取物和胰蛋白胨提供氮源和生長因子。丙酸鈉、 甘氨酸和高濃度甘露醇刺激目的菌的生長和修復，氯化鋰是選擇性抑制劑，抑制革蘭氏陰性桿菌，亞碲 酸鉀與甘氨酸抑制除葡萄球菌外其它革蘭氏陽性菌，尤其是芽孢桿菌生長。厭氧培養可增強選擇性，在 培養基表面加2cm深石蠟油能夠抑制微球菌的生長。亞碲酸鹽同時也是鑒別指示劑，金黃色葡萄球菌能夠 還原亞碲酸鹽，生成黑色的碲。如果培養基顏色變黑，則認為是金葡菌陽性。將菌液接種到金葡菌分離 培養基如Baird-Parker瓊脂上。
Giolitti-Cantoni肉湯的用途：
改良Giolitti-Cantoni肉湯培養基（Modified Giolitti and Cantoni Broth ）被ISO 6888-3:2003規定的MPN法檢測和增菌食品和飼料中的凝固酶陽性葡萄球菌用培 養基。
本培養基是回收受傷葡萄球菌或從低含量樣本中回收葡萄球菌的最佳培養基。
Giolitti-Cantoni肉湯培養基配方與配制方法
| 培養基成分： | 含量：g/L |
|---|---|
| 胰蛋白胨 | 10.0 |
| 酵母提取物 | 5.0 |
| 牛肉提取物 | 5.0 |
| 氯化鋰 | 5.0 |
| 甘露醇 | 20.0 |
| 氯化鈉 | 5.0 |
| 甘氨酸 | 1.2 |
| 丙酸鈉 | 3.0 |
| pH | 6.9± 0.2 |
配制方法：
1. 稱取54.2g本品，加入1L去離子水，1ml吐溫-80，溫和加熱溶解。
2. 分裝19ml至20 x 200mm試管中。或者分裝9ml至16 x 160mm試管中。
3. 121°C滅菌15min。
4. 快速冷卻，加入0.1ml無菌1%亞碲酸鉀溶液到9ml培養基中（工作濃度0.1g/L）。
實驗方法
The medium should be inoculated as soon as it has been cooled after autoclaving. If there is a delay in putting the medium to use it must be freed from dissolved air by immersion in free-flowing steam for 20 minutes.
Inoculate 1g of sample material and 1ml aliquots of a series of suitable decimal dilutions into tubes containing 19 ml of Giolitti-Cantoni Broth. Two tubes are used for the sample material and for each of the dilutions. This increases the likelihood of detecting Staphylococcus aureus when it is present in very small numbers.
The medium is overlaid with 2cm of molten sterile paraffin wax (melting temperature 42 -44°C) and incubated for 48 hours at 35°C, examining daily. The result is considered negative for Staphylococcus aureus if no blackening of the medium is observed. If blackening does occur at the bottom of the tubes or general blackening of the medium, the broth is streaked on to a staphylococcal isolation medium, such as Baird-Parker Medium8 and incubated at 35°C for 24-48 hours. The result is considered positive if black colonies, with a narrow white margin, surrounded by a zone of clearing, are seen.
結果與分析
標準菌株 生長情況
金葡菌 ATCC25923 生長極好，培養基變黑