LOCUS       Exported               14787 bp DNA     circular SYN 18-JUL-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14787)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 14787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14787
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /note="color: #993366"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
                     /note="color: #a6acb3"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /note="color: #993366"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        2993..3338
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff"
     CDS             3600..3833
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /note="color: #993366"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     rep_origin      complement(4164..4788)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
                     /note="color: #ffff00"
     promoter        5158..5341
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
                     /note="color: #ffffff"
     CDS             5394..6404
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /note="color: #993366"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRP"
     terminator      6457..6709
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
     promoter        6929..6975
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff"
     CDS             7010..7504
                     /label=avrPto
                     /note="color: #993366; direction: RIGHT"
     misc_feature    complement(8651..8675)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(10274..11419)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /note="color: #993366"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11721..12512)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /note="color: #993366"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12704..13471
                     /label=IS1
                     /note="prokaryotic transposable element"
                     /note="color: #a6acb3"
     rep_origin      complement(14172..14782)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
                     /note="color: #ffff00"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttcctgcagg tccccagatt agccttttca
     2521 atttcagaaa gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca
     2581 agacgatcta cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag
     2641 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca
     2701 agatcagaag tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag
     2761 taatagagat tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa
     2821 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
     2881 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
     2941 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
     3001 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
     3061 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
     3121 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     3181 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     3241 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     3301 cctctatata aggaagttca tttcatttgg agagaacacg ggggactcta gtttaaacaa
     3361 tggcagatcc aatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
     3421 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     3481 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
     3541 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct aggtcgagcg
     3601 cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga
     3661 tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc
     3721 cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact
     3781 tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt ggggatccaa
     3841 ttcagcaagc cactgcagga gtctcacaag acacttcgga aaatcctaac aaaacaatag
     3901 ttcctgctgc attaccacag ctcaccccta ccttggtgtc actgctggag gtgattgaac
     3961 ccgaggtgtt gtatgcagga tatgatagct ctgttccaga ttcagcatgg agaattatga
     4021 ccacactcaa catgttaggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcga
     4081 taccaggctt cagaaactta cacctggatg accaaatgac cctgctacag tactcatgga
     4141 tgtttctcat ggcatttgcc ctgggttgga gatcatacag acaatcaagt ggaaacctgc
     4201 tctgctttgc tcctgatctg attattaatg agcagagaat gtctctaccc tgcatgtatg
     4261 accaatgtaa acacatgctg tttgtctcct ctgaattaca aagattgcag gtatcctatg
     4321 aagagtatct ctgtatgaaa accttactgc ttctctcctc agttcctaag gaaggtctga
     4381 agagccaaga gttatttgat gagattcgaa tgacttatat caaagagcta ggaaaagcca
     4441 tcgtcaaaag ggaagggaac tccagtcaga actggcaacg gttttaccaa ctgacaaagc
     4501 ttctggactc catgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt
     4561 tggataagac catgagtatt gaattcccag agatgttagc tgaaatcatc actaatcaga
     4621 taccaaaata ttcaaatgga aatatcaaaa agcttctgtt tcatcaaaaa tgactcgacc
     4681 taactgagta agctagcttg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac
     4741 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa
     4801 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta
     4861 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc
     4921 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata
     4981 tgaggagtaa aacactagat ccccaaacaa gcttgaaact gaaggcggga aacgacaatc
     5041 tgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa
     5101 gccgttttac gtttggaact gacagaaccg caacgattga aggagccact cagccgcggg
     5161 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
     5221 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
     5281 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc
     5341 accggatccg ctagaggatc tcgaccctgc aagatcccgg ggggcaatga gatatgaaaa
     5401 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     5461 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     5521 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     5581 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     5641 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     5701 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     5761 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     5821 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5881 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5941 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     6001 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     6061 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     6121 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     6181 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     6241 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     6301 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     6361 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgggatct tggaggtgat
     6421 gtaacatgat cacaagctga tcccccgaat ttccccgatc gttcaaacat ttggcaataa
     6481 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     6541 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     6601 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     6661 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgatc
     6721 ccccctcgac agcttgcatg ccggtcgact ttagaggatc cgggtgacag ccctccgacg
     6781 ggtgacagcc ctccgacggg tgacagccct ccgaattcta gaggatccgg gtgacagccc
     6841 tccgacgggt gacagccctc cgacgggtga cagccctccg aattcgagct cggtacccgg
     6901 ggatctgtcg acctcgatcg agatcttcgc aagacccttc ctctatataa ggaagttcat
     6961 ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgagacca tgggaaatat
     7021 atgtgtcggc ggatccagga tggcccatca ggtgaactcc ccagaccgag ttagtaacaa
     7081 ctcgggtgac gaagataacg taacgtccag tcaactgctg agcgtcagac atcaacttgc
     7141 ggagtctgct ggtgtaccaa gagatcagca tgaatttgtt agtaaccaag cacctcaaag
     7201 cctgagaaat cgctacaaca atctttactc acatacgcaa agaacactgg atatggcgga
     7261 catgcagcat aggtacatga cgggagcgtc aggaatcaat ccgggaatgc tgccacatga
     7321 gaatgtggac gatatgcgta gcgctataac tgattggagt gacatgcgcg aagctctgca
     7381 gtacgcaatg ggtatccatg ccgacatccc accgtctcca gagcgatttg ttgcgactat
     7441 gaacccgaac ggatcaattc gaatgtcaac actttctcct agcccgtacc gtaactggca
     7501 atgaactagt cgatccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
     7561 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
     7621 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
     7681 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
     7741 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
     7801 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
     7861 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
     7921 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
     7981 gctttttgtg tcgaaggggt accgagtcga gaattcactg gccgtcgttt tacaacgtcg
     8041 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     8101 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     8161 gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8281 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     8341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8401 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     8461 tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     8521 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     8581 gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     8641 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     8701 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     8761 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     8821 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     8881 gtaacgatga cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga
     8941 attatcagcc ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact
     9001 atgccgacat aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt
     9061 ctttagaagt gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag
     9121 gtcggggacc cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat
     9181 ctggggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
     9241 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
     9301 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
     9361 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
     9421 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
     9481 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
     9541 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
     9601 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
     9661 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
     9721 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
     9781 gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg
     9841 aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc
     9901 gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt
     9961 tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct
    10021 ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca
    10081 agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt
    10141 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
    10201 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
    10261 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
    10321 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
    10381 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
    10441 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
    10501 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
    10561 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
    10621 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
    10681 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
    10741 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
    10801 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
    10861 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
    10921 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
    10981 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
    11041 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
    11101 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
    11161 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
    11221 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
    11281 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
    11341 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
    11401 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
    11461 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
    11521 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
    11581 tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct
    11641 gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt
    11701 tgcgccacat ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    11761 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    11821 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    11881 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    11941 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    12001 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    12061 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    12121 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    12181 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    12241 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    12301 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    12361 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    12421 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    12481 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    12541 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    12601 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    12661 ataacatagt atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt
    12721 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtg tctatcagct
    12781 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
    12841 agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt
    12901 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
    12961 caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
    13021 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
    13081 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
    13141 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
    13201 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
    13261 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
    13321 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
    13381 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
    13441 aaaacactat caataagttg gagtcattac ccaattatga tagaatttac aagctataag
    13501 gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat
    13561 agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat
    13621 atcttctata taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg
    13681 cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga
    13741 gtaattctgt aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct
    13801 caaatggttc gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat
    13861 gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac
    13921 ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc
    13981 gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct
    14041 gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat
    14101 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc
    14161 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg
    14221 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt
    14281 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc
    14341 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc
    14401 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat
    14461 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc
    14521 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct
    14581 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc
    14641 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt
    14701 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
    14761 gcccagcggc gagggcaacc agcccgg
//
