| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 載體名稱: | pBTL-2 |
| 質粒類型: | 廣宿主載體；克隆載體；原核表達載體 |
| 高拷貝/低拷貝: | -- |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | lac |
| 載體大小: | 2618 bp |
| 5' 測序引物及序列: | -- |
| 3' 測序引物及序列: | -- |
| 載體標簽: | -- |
| 載體抗性: | Kanamycin |
| 克隆菌株: | DH5α 等 |
| 宿主細胞（系）: | 多個菌屬 |
| 備注: | -- |
| 產品目錄號: | -- |
| 組成型/誘導型: | 誘導型 |
買家導航
pBTL-2載體質粒基本信息
pBTL-2質粒圖譜載體圖譜和pBTL-2載體序列質粒序列多克隆位點信息
pBTL-2質粒載體簡介
pBTL-2質粒序列載體序列
LOCUS DQ058740 2618 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBTL-2, complete sequence.
ACCESSION DQ058740
VERSION DQ058740.1 GI:69048456
KEYWORDS .
SOURCE Broad host range vector pBTL-2
ORGANISM Broad host range vector pBTL-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2618)
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Broad host range vectors for stable genomic library construction
JOURNAL Biotechnol. Bioeng. 94 (1), 151-158 (2006)
PUBMED 16496398
REFERENCE 2 (bases 1 to 2618)
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..2618
/organism="Broad host range vector pBTL-2"
/mol_type="other DNA"
/db_xref="taxon:334972"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99749.1"
/db_xref="GI:69048457"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..2446
/gene="kan"
/note="kanamycin resistance gene from TN903"
CDS 1631..2446
/gene="kan"
/codon_start=1
/transl_table=11
/product="kanamycin kinase"
/protein_id="AAY99750.1"
/db_xref="GI:69048458"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
regulatory 2463..2494
/regulatory_class="terminator"
/note="TonB terminator"
regulatory 2521..2603
/regulatory_class="promoter"
/note="lac promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga
1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct
2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt
2521 ttgcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
2581 gtgagcggat aacaatttca cactctagag aattcgtc
//