pDEST12.2

價格：2500元

聯系方式：I47-825O-882O

買家導航

pDEST12.2載體質粒基本信息

出品公司:	Invitrigen
載體名稱:	pDEST12.2
質粒類型:	Gateway載體；哺乳動物細胞表達載體
高拷貝/低拷貝:	高拷貝
啟動子:	CMV
克隆方法:	Gateway
載體大小:	7278 bp
5' 測序引物及序列:	SP6 Forward ATTTAGGTGACACTATAG
3' 測序引物及序列:	T7 Reverse GCTAGTTATTGCTCAGCGG
載體標簽:	無標簽
載體抗性:	氨芐青霉素,氯霉素（僅空載體）
真核篩選標記:	新霉素（Neomycin）
克隆菌株:	ccdB陽性篩選 DB3.1 感受態細胞，陰性篩選用 OmniMAX2-T1 感受態細胞
宿主細胞（系）:	常規細胞系，293、CV-1、CHO等
備注:	--
產品目錄號:	--
穩定性:	穩表達或瞬表達
組成型/誘導型:	組成型
病毒/非病毒:	非病毒

pDEST12.2質粒圖譜載體圖譜和pDEST12.2載體序列質粒序列多克隆位點信息

pDEST12.2載體圖譜

pDEST12.2質粒載體簡介

Gateway哺乳動物表達載體選擇指南：

pDEST12.2   不帶標簽

pDEST26     N-His標簽 G418篩選

pDEST27   N-GST標簽 G418篩選

pDEST40   C-His標簽、C-V5標簽   G418篩選

pcDNA-DEST40   C-His標簽、C-V5標簽   G418篩選

pcDNA-DEST47 C-綠色熒光蛋白 G418篩選

pcDNA-DEST53 N-綠色熒光蛋白 G418篩選

pcDNA3.2/V5-DEST C-V5標簽 G418篩選

pcDNA6.2/V5-DEST C-V5標簽 Blasticidin S篩選

pcDNA6.2/N-YFP-DEST N-黃色熒光蛋白 Blasticidin S篩選

pcDNA6.2/N-EmGFP-DEST N-綠色熒光蛋白 Blasticidin S篩選

pcDNA6.2/C-YFP-DEST C-黃色熒光蛋白 Blasticidin S篩選

pcDNA6.2/C-EmGFP-DEST C-綠色熒光蛋白 Blasticidin S篩選

pcDNA6.2/BioEase-DEST N-生物素 Blasticidin S篩選

pDEST12.2質粒序列載體序列

LOCUS       pDEST12.2	7278 bp 	DNA	SYN
DEFINITION  pDEST12.2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="pDEST12.2"
                     /mol_type="other DNA"
     misc_feature    19..306
                     /label="CAG_enhancer"
     promoter        24..516
                     /label="CMV_immearly_promoter"
     misc_feature    473..493
                     /label="CMV_fwd_primer"
     promoter        474..543
                     /label="CMV_promoter"
     promoter        486..605
                     /label="CMV2_promoter"
     misc_feature    517..536
                     /label="pCEP_fwd_primer"
     misc_feature    519..543
                     /label="LNCX_primer"
     misc_feature    687..704
                     /label="Sp6_primer"
     misc_feature    730..854
                     /label="attR1"
     misc_feature    complement(754..854)
                     /label="attR2"
     promoter        879..902
                     /label="lac_promoter"
     gene            963..1622
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             963..1622
                     /label="ORF frame 3"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    1964..2269
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(2310..2417)
                     /label="attR1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    2310..2410
                     /label="attR2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     promoter        complement(2466..2484)
                     /label="T7_promoter"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     promoter        complement(2495..2511)
                     /label="M13_forward20_primer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(2504..2526)
                     /label="M13_pUC_fwd_primer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    2618..2633
                     /label="SV40_int"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     terminator      2919..3050
                     /label="SV40_PA_terminator"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    3007..3026
                     /label="EBV_rev_primer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     rep_origin      3193..3499
                     /label="f1_origin"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(3773..3793)
                     /label="pBABE_3_primer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    complement(3779..3994)
                     /label="SV40_enhancer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     promoter        3791..4059
                     /label="SV40_promoter"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     rep_origin      3958..4035
                     /label="SV40_origin"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    4020..4039
                     /label="SV40pro_F_primer"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     CDS             4158..4952
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            4161..4949
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(4467..5003)
                     /label="ORF frame 2"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      5016..5064
                     /label="synth_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    5224..5246
                     /label="pGEX_3_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     promoter        5405..5433
                     /label="AmpR_promoter"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     gene            5475..6335
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             5475..6335
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6490..7109
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 TCGCGAATGC ATGTCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC
   61 GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT
  121 TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA
  181 GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG
  241 CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA
  301 GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG
  361 TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG
  421 CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG
  481 GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG
  541 ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC
  601 AGCCTCCGGA CTCTAGCCTA GGCCGCGGGA CGGATAACAA TTTCACACAG GAAACAGCTA
  661 TGACCATTAG GCCTTTGCAA AAAGCTATTT AGGTGACACT ATAGAAGGTA CGCCTGCAGG
  721 TACCGGATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
  781 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
  841 ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
  901 CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA
  961 AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG
 1021 AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG
 1081 ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA
 1141 TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG
 1201 GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG
 1261 AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT
 1321 ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG
 1381 AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG
 1441 TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG
 1501 GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC
 1561 ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT
 1621 AAACGCGTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT
 1681 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC
 1741 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA
 1801 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG
 1861 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT
 1921 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA
 1981 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA
 2041 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT
 2101 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC
 2161 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG
 2221 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC
 2281 CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT
 2341 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
 2401 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATCGC GTGCATGCGA CGTCATAGCT
 2461 CTCTCCCTAT AGTGAGTCGT ATTATAAGCT AGGCACTGGC CGTCGTTTTA CAACGTCGTG
 2521 ACTGGGAAAA CTGCTAGCTT GGGATCTTTG TGAAGGAACC TTACTTCTGT GGTGTGACAT
 2581 AATTGGACAA ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG
 2641 TATAATGTGT TAAACTAGCT GCATATGCTT GCTGCTTGAG AGTTTTGCTT ACTGAGTATG
 2701 ATTTATGAAA ATATTATACA CAGGAGCTAG TGATTCTAAT TGTTTGTGTA TTTTAGATTC
 2761 ACAGTCCCAA GGCTCATTTC AGGCCCCTCA GTCCTCACAG TCTGTTCATG ATCATAATCA
 2821 GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA
 2881 ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG
 2941 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
 3001 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC GATCCTGCAT
 3061 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGCTGGCG TAATAGCGAA
 3121 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG
 3181 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
 3241 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
 3301 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
 3361 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
 3421 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
 3481 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
 3541 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAT ATTTAACGCG
 3601 AATTTTAACA AAATATTAAC GTTTACAATT TCGCCTGATG CGGTATTTTC TCCTTACGCA
 3661 TCTGTGCGGT ATTTCACACC GCATACGCGG ATCTGCGCAG CACCATGGCC TGAAATAACC
 3721 TCTGAAAGAG GAACTTGGTT AGGTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG
 3781 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
 3841 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
 3901 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
 3961 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT
 4021 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC
 4081 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTCTT CTGACACAAC AGTCTCGAAC
 4141 TTAAGGCTAG AGCCACCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT
 4201 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG
 4261 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG
 4321 GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG
 4381 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG
 4441 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA
 4501 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC
 4561 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC
 4621 AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA
 4681 AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA
 4741 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG
 4801 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG
 4861 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG
 4921 CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA
 4981 CCAAGCGACG CCCAACCTGC CATCACGATG GCCGCAATAA AATATCTTTA TTTTCATTAC
 5041 ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGCGATAAG GATCCGCGTA TGGTGCACTC
 5101 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG
 5161 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
 5221 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA
 5281 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA
 5341 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA
 5401 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT
 5461 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG
 5521 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
 5581 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG
 5641 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG
 5701 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT
 5761 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA
 5821 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
 5881 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC
 5941 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC
 6001 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC
 6061 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG
 6121 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
 6181 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA
 6241 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG
 6301 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA
 6361 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT
 6421 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC
 6481 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
 6541 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
 6601 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG
 6661 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC
 6721 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG
 6781 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
 6841 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT
 6901 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG
 6961 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
 7021 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC
 7081 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
 7141 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG
 7201 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA
 7261 GCGAGGAAGC GGAAGAGC
//

pDEST12.2載體圖譜質粒圖譜pdf版和pDEST12.2載體序列質粒序列等相關資料下載

pDEST12.2質粒載體應用舉例

上一篇：pLenti6.2/V5-DEST
下一篇：pENTR221

pcDNA5/FRT/V5-His-TOPO	pRSET-EGFP
pUCTag	pProLabel-C
pCL-Ampho	pNEO-ars
pOM2	pET-12c(+)
pNEO	pBT-4
pPIC9	pcDNA6.2-DsRed2-MCS1 miR
pALEX a,b,c	pRB373
pML21	pcDNA3.1-GP130v1
pMT-Bip-V5-HisA	pcDNA3.1(+)/CAT
pAGO	Pgex-HRV3C
pTet-Splice	pSC101-EcoRImeth+
pDC515	pEF6/myc-His B
pHY15	pBAD18
pKLAC1	pIC20H
pTP-4	ISceI-GR-RFP
pSH65	pAN7-1
pFA1	pET-41a(+)
pCREj-2	pL-brevisGH3-MBP
c-Flag pcDNA3	pPIC9k-His
pGL4.72[hRlucCP]	pSET152
pHS85	pUG6
pMal-c2G	pBad43
pdKeima-Red-S1	pLNCX2
pcDNA5/FRT	pCRE-DD-AmCyan1
pENTR11-Dual	pLexm
pOrf681_TA	pSUMO
pLVX-DD-ZsGreen1 Control	pIJ702-del35-55
EWD299	pET-21a(+)
pEF1/V5-HisA	pATPase_TA
pAcGFP1-N2	pET-32 EK/LIC
pDsRed-Monomer	pET-32a(+)
pCDH-CMV-MCS-EF1a-RFP+BSD	pBD-NF-kB
pWF1	pSP72
pHB-1	pLLP-OmpA
pSC101	pHV23
pMDLg/pRRE	pACT-MyoD
pCYM1	pSUPER.neo+gfp
pBluescript II KS(-)	pDEST26
pVPack-GP	pGL4.45[luc2P/ISRE/Hygro]
pMTD1.3xA	pFLAG-CMV2
pCH2	pTi
PX543	pcDNA4/HisMax B
pCas9D10A-GFP	pBBR1MCS-2
pFLAG-CMV-3	pET-28a-BFP-C
pGL4.38[luc2P/p53 RE/Hygro]	pCRE-MetLuc2-Reporter
pGL4.41[luc2P/HSE/Hygro]	pAD-SV40T
pJSC73	pIκB-EGFP
pREP4	pCGN978
pHSG422	pPIC ZαGB
pUC4-KISS	pMal-c4X

pCMV-MMLVgag-3xNES-ABE8e	pCAS9-mCherry empty
pUCC001	pLenti-EML4-ALK variant 3a
EC1000	pRC-CMV-Rev1b
pcDNA3.4_7C11-1 heavy chain	TAL3393
pNeae2	pM1s3AsR_TS-C7
pGL3 2 kb prom. CD274	pLJC5-3XHA-EGFP-PEX26
pAraGFPCDF	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
pXAT2	pGH335_MS2-AID*Δ-Hygro
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	pLVX-M-puro
pPPEM	LeGO-G
800 pSG5L HA PTEN wt	BPK3082
pEGFP-puro	pNEU-hMbPylRS-4xU6M15
pM-Cas9	pMSCV-IRES-GFP II (pMIG II)
pQUAST>stop>mCD8-GFP	pMSCV-hygro-STING
pAAV2/8	Cbh_v5 AAV-CBE N-terminal
pAAVS1-PC-GCaMP6f	pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV
pJSC131 - Bacterial expression plasmid f	pFC334
pMXs-hOCT4 (Plath)	pSELECT-HA-mFOXO1
CKS2 gRNA (BRDN0001149143)	pT3-Neo-EF1a-GFP
pLenti-DsRed_IRES_MAPT:EGFP	pTRKH2
pCAG-GBP1-10gly-lexADBD	AbVec1.1-IGKC
pBHt2	pCS2TAL3-RR
c-myc-PT3EF1a	pCW-Cas9
Lenti-luciferase-P2A-Neo	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
pAAV-EFS-CjCas9-eGFP-HIF1a	pEGFP-LC3
phage UbiC NLS HA stdMCP stdHalo	NM9-SpCas9-NLS3
pJKR-L-tetR	pT4-CMV-GFP
pLenti X2 Puro DEST (w16-1)	pSR47
LB1366 (Bcl-2 promoter with MHS1234)	pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
pSBtet-GP	pLentiV2-dCas9-VP64
pCS2TAL3-DD	401 pSG5L
ilux pGEX(-)	pC003 - LshC2C2 locus into pACYC184 for
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	pTEF:ATP
PB_tre_Cas9	pAc-Neo-OVA
pCDH-puro-Bcl-XL	FUmGW
pICH86966::AtU6p::sgRNA_PDS	pNN9
pAAV-EF1a-FLEX-GTB	pTRE Tight ArchT-GFP
pMonAID_nCas9-PmCDA_Hyg_ALS	p201G Cas9
pDIRECT_23D	VVT1
pMal-T-Avi-His/BirA	pAc-sgRNA-Cas9
3XMEF2-luc	pGGA008
p58sheLL	pMJ329: pCMV-MmeFz2
pAC1404-pCR8-mRuby2_NLSPUFa	1306 pSG5L HA FKHR wt
pCXLE-hOCT3/4	pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-
BECLIN-HA WT	EC2_3_dCas9_Mxi1_sgRNA
pYES-mtGFP	EC2_10_dCas9_VPR_HC_sgRNA
Tet-O-FUW-Neurod1	GST-HA-GFP11-N1
pRAD1	pHAGE-PTCH1
pAAVS1-PDi-CRISPRn	pET28a-At-TRL
pCMV-hA3G-BE3	pNK9

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pDEST12.2

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