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pENTR-L1-pLac-lacZa-R5載體質粒基本信息
pENTR-L1-pLac-lacZa-R5質粒圖譜載體圖譜和pENTR-L1-pLac-lacZa-R5載體序列質粒序列多克隆位點信息
pENTR-L1-pLac-lacZa-R5質粒載體簡介
pENTR-L1-pLac-lacZa-R5質粒序列載體序列
LOCUS Exported 2968 bp ds-DNA circular SYN 13-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pENTR-L1-pLac-lacZa-R5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2968)
AUTHORS Biofeng Lab.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..2968
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 694..724
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 732..748
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 756..772
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
gene 767..983
/note="lacZ-alpha"
misc_feature 785..841
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(842..858)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1031..1154
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR5"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1210..1228)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1233..1249)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 1362..2171
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2318..2906
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agcaggctag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
721 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
781 cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
841 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
901 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
961 gcccttccca acagttgcgc agctaacact ataaaaaggc agtgatgcag acggggcttt
1021 tttatgcagg caactttgta tacaaaagtt gaacgagaaa cgtaaaatga tataaatatc
1081 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
1141 atccagtcac tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc
1201 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
1261 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
1321 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
1381 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
1441 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
1501 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
1561 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
1621 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
1681 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
1741 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
1801 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
1861 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
1921 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
1981 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
2041 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
2101 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
2161 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
2221 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
2941 ccttttgctg gccttttgct cacatgtt
//