LOCUS       pSE380	4476 bp 	DNA	SYN
DEFINITION  pSE380
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="pSE380"
                     /mol_type="other DNA"
     promoter        202..275
                     /label="trc_promoter"
     misc_feature    244..266
                     /label="M13_pUC_rev_primer"
     misc_feature    complement(659..676)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(659..676)
                     /label="pBAD_rev_primer"
     terminator      709..866
                     /label="rrnB_terminator"
     terminator      832..875
                     /label="rrnB_T1_terminator"
     terminator      1007..1034
                     /label="rrnB_T2_terminator"
     promoter        1076..1104
                     /label="AmpR_promoter"
     gene            1146..2006
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1146..2006
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2161..2780
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3177..3199
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3346..4437
                     /label="lacI"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             3478..4437
                     /label="ORF frame 1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
ORIGIN
    1 aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag
   61 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
  121 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
  181 caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggaatt
  241 gtgagcggat aacaatttca cacaggaaac agaccatggc tggtgaccac gtcgtggaat
  301 gccttcgaat tcagcacctg cacatgggac gtcgacctga ggtaattata acccgggccc
  361 tatatatgga tccaattgca atgatcatca tgacagatct gcgcgcgatc gatatcagcg
  421 ctttaaattt gcgcatgcta gctatagttc tagaggtacc ggttgttaac gttagccggc
  481 tacgtatact ccggaatatt aataggccta ggatgcatat ggcggccgcc tgcagctggc
  541 gccatcgata cgcgtacgtc gcgaccgcgg acatgtacag agctcgagaa gtactagtgg
  601 ccacgtgggc cgtgcacctt aagcttggct gttttggcgg atgagagaag attttcagcc
  661 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
  721 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
  781 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
  841 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
  901 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
  961 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
 1021 acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
 1081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
 1141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
 1201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
 1261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
 1321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
 1381 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
 1441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
 1501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
 1561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
 1621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
 1681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
 1741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
 1801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
 1861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
 1921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
 1981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
 2041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
 2101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
 2161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
 2221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
 2281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
 2341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
 2401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
 2461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
 2521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
 2581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
 2641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
 2701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
 2761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
 2821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
 2881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
 2941 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
 3001 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
 3061 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
 3121 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
 3181 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
 3241 ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gcgcaaaacc
 3301 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
 3361 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
 3421 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
 3481 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
 3541 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
 3601 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
 3661 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
 3721 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
 3781 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
 3841 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
 3901 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
 3961 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
 4021 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
 4081 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
 4141 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
 4201 atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
 4261 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
 4321 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
 4381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
 4441 caacgcaatt aatgtgagtt agcgcgaatt gatctt
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