| 出品公司: | Invitrogen |
|---|---|
| 別名: | pTrcHisC, pTrcHis C |
| 質粒類型: | 大腸桿菌蛋白表達載體 |
| 表達水平: | 高拷貝 |
| 啟動子: | Trc |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 4412 bp |
| 5' 測序引物及序列: | pTrcHis-F: gaggtatatattaatgtatcg |
| 3' 測序引物及序列: | pTrcHis-R:GATTTAATCTGTATCAGG |
| 載體標簽: | N-His, N-EK |
| 載體抗性: | Ampicillin |
| 備注: |
Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420.
|
| 產品目錄號: | V360-20 |
| 穩定性: | 瞬時表達 Transient |
| 組成型: | 組成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
買家導航
pTrcHis C載體 pTrcHisC質粒基本信息
pTrcHis C質粒圖譜 pTrcHisC載體圖譜和pTrcHis C載體序列 pTrcHisC質粒序列多克隆位點信息
pTrcHis C質粒 pTrcHisC載體簡介
pTrcHis載體是來由pBR322質粒衍生而來的表達載體,設計用來在大腸桿菌中進行重組蛋白的高效表達和純化。
載體上的Trc啟動子和rrnB抗終止區使得高水平蛋白表達成為可能.Trc啟動子由Trp啟動子的-35區域和Lac啟動子的-10區域共同組成。pTrcHis載體同時包含有一個LacIq基因，該基因編碼了Lac啟動子的抑制蛋白。LacIq基因的存在使得宿主菌是LacIq+還是LacIq-都可以高效抑制插入基因的轉錄。
進行蛋白表達時，大腸桿菌需要生長到對數生長期，然后加入1mM IPTG，去除阻遏蛋白，誘導表達。載體上的迷你順反子結構能夠增強插入基因的轉錄水平，從而高水平表達目的蛋白。DNA插入片段位于載體表達片段下游，在其上游有一個N-端融合表達多肽序列，該序列包含有ATG起始密碼子，6xHis標簽，T7噬菌體10號基因的翻譯增強子，X press抗原表位，腸激酶酶切識別位點。
pTrcHis C質粒序列 pTrcHisC載體序列
LOCUS pTrcHisC 4412 bp DNA circular SYN
DEFINITION pTRCHISC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|gt12365.cn|
FEATURES Location/Qualifiers
source 1..4412
/organism="pTRCHISC"
/mol_type="other DNA"
misc_feature 235..257
/label="M13_pUC_rev_primer"
misc_feature 264..333
/label="rrnB_ATS"
misc_feature 370..390
/label="pTrcHis_fwd_primer"
misc_feature 383..409
/label="minicis"
misc_feature 445..463
/label="Xpress_fwd_primer"
misc_feature 446..478
/label="T7_leader"
misc_feature 482..505
/label="Xpress_EK"
misc_feature complement(595..612)
/label="pBAD_rev_primer"
misc_feature complement(595..612)
/label="pTrcHis_rev_primer"
terminator 645..802
/label="rrnB_terminator"
terminator 768..811
/label="rrnB_T1_terminator"
terminator 943..970
/label="rrnB_T2_terminator"
promoter 1012..1040
/label="AmpR_promoter"
gene 1082..1942
/label="Ampicillin"
/gene="Ampicillin"
CDS 1082..1942
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2097..2716
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3113..3135
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3282..4373
/label="lacI"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 3414..4373
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
ORIGIN
1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
421 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
481 ggatctgtac gacgatgacg ataaggatcg atggatccga cctcgagatc tgcagatggt
541 accatatggg aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
601 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
661 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
721 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
841 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
901 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
961 atggcctttt tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat
1021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
1081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
1141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
1201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
1261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
1321 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
1381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
1441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
1501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
1561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
1621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
1681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
1741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
1801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
1861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
1921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
1981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
2101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2221 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
2281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
2641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
2701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
2761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
2821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
2881 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
2941 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
3001 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
3061 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
3121 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
3181 cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggcgc aaaacctttc
3241 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
3301 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
3361 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
3421 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
3481 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
3541 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
3601 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
3661 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
3721 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
3781 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
3841 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
3901 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
3961 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
4021 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
4081 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
4141 cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
4201 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
4261 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
4321 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
4381 gcaattaatg tgagttagcg cgaattgatc tg
//
DEFINITION pTRCHISC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|gt12365.cn|
FEATURES Location/Qualifiers
source 1..4412
/organism="pTRCHISC"
/mol_type="other DNA"
misc_feature 235..257
/label="M13_pUC_rev_primer"
misc_feature 264..333
/label="rrnB_ATS"
misc_feature 370..390
/label="pTrcHis_fwd_primer"
misc_feature 383..409
/label="minicis"
misc_feature 445..463
/label="Xpress_fwd_primer"
misc_feature 446..478
/label="T7_leader"
misc_feature 482..505
/label="Xpress_EK"
misc_feature complement(595..612)
/label="pBAD_rev_primer"
misc_feature complement(595..612)
/label="pTrcHis_rev_primer"
terminator 645..802
/label="rrnB_terminator"
terminator 768..811
/label="rrnB_T1_terminator"
terminator 943..970
/label="rrnB_T2_terminator"
promoter 1012..1040
/label="AmpR_promoter"
gene 1082..1942
/label="Ampicillin"
/gene="Ampicillin"
CDS 1082..1942
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2097..2716
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3113..3135
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3282..4373
/label="lacI"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 3414..4373
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
ORIGIN
1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
421 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
481 ggatctgtac gacgatgacg ataaggatcg atggatccga cctcgagatc tgcagatggt
541 accatatggg aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
601 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
661 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
721 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
841 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
901 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
961 atggcctttt tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat
1021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
1081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
1141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
1201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
1261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
1321 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
1381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
1441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
1501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
1561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
1621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
1681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
1741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
1801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
1861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
1921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
1981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
2101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2221 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
2281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
2641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
2701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
2761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
2821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
2881 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
2941 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
3001 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
3061 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
3121 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
3181 cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggcgc aaaacctttc
3241 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
3301 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
3361 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
3421 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
3481 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
3541 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
3601 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
3661 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
3721 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
3781 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
3841 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
3901 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
3961 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
4021 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
4081 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
4141 cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
4201 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
4261 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
4321 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
4381 gcaattaatg tgagttagcg cgaattgatc tg
//
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