| 出品公司: | GenScript |
|---|---|
| 載體名稱: | pUC57-Kan |
| 質粒類型: | 大腸桿菌載體；克隆質粒 |
| 高拷貝/低拷貝: | -- |
| 復制子: | pUC |
| 啟動子: | Lac/lac |
| 載體大小: | 2579bp |
| 5' 測序引物及序列: | M13R:CAGGAAACAGCTATGACC |
| 3' 測序引物及序列: | M13F:TGTAAAACGACGGCCAGT |
| 載體標簽: | LacZ |
| 載體抗性: | 卡那霉素 |
| 篩選標記: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主細胞（系）: | -- |
| 備注: | -- |
| 產品目錄號: | -- |
| 穩定性: | -- |
| 組成型/誘導型: | -- |
| 病毒/非病毒: | 非病毒 |
買家導航
pUC57-Kan載體 pUC57Kan質粒基本信息
pUC57-Kan質粒圖譜 pUC57Kan載體圖譜和pUC57-Kan載體序列 pUC57Kan質粒序列多克隆位點信息
pUC57-Kan質粒 pUC57Kan載體簡介
pUC57-Kan質粒序列 pUC57Kan載體序列
LOCUS pUC57-Kan 2579 bp ds-DNA circular SYN 25-NOV-2013
DEFINITION Cloning vector with a kanamycin resistance marker, suitable for
generating ExoIII deletions.
ACCESSION .
VERSION .
KEYWORDS pUC57-Kan
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2579)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Saturday, August 1, 2020 from SnapGene Viewer 3.1.4
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..2579
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
CDS complement(146..493)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLHAGLCSRRARDPISRCIREVPSSNSLAVVLQRRDWEN
PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIW
CTLSTICSDAA"
primer_bind 379..395
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 396..476
/note="MCS"
/note="multiple cloning site"
primer_bind complement(489..505)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 513..529
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(537..567)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(891..1479)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1657..2466)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg
481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaagccca
1621 atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
1681 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
1741 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
1801 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
1861 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
1921 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
1981 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
2041 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
2101 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
2161 ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
2221 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
2281 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
2341 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
2401 atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg
2461 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
2521 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgca
//
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