| 出品公司: | -- |
|---|---|
| 載體名稱: | pZsYellow |
| 質粒類型: | 大腸桿菌表達載體 |
| 高拷貝/低拷貝: | -- |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | lac |
| 載體大小: | 3331 bp |
| 5' 測序引物及序列: | -- |
| 3' 測序引物及序列: | -- |
| 載體標簽: | ZsYellow (Cterm) |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | -- |
| 克隆菌株: | -- |
| 宿主細胞（系）: | -- |
| 備注: | -- |
| 產品目錄號: | -- |
| 穩定性: | -- |
| 組成型/誘導型: | -- |
| 病毒/非病毒: | -- |
買家導航
pZsYellow載體質粒基本信息
pZsYellow質粒圖譜載體圖譜和pZsYellow載體序列質粒序列多克隆位點信息
pZsYellow質粒載體簡介
pZsYellow質粒序列載體序列
LOCUS pZsYellow 3331 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for expressing ZsYellow1 in bacteria.
ACCESSION .
VERSION .
KEYWORDS pZsYellow
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3331)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Thursday, June 25, 2020 from SnapGene Viewer 3.1.4
//www.snapgene.com
COMMENT Translation from the lacZ start codon produces a fusion protein in
E. coli.
FEATURES Location/Qualifiers
source 1..3331
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 143..173
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 217..219
/codon_start=1
/product="lacZ start codon"
/note="ATG"
/translation="M"
CDS 289..984
/codon_start=1
/product="Zoanthus yellow fluorescent protein"
/label=ZsYellow
/note="ZsYellow"
/translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
AIAFPSALA"
promoter 1426..1530
/gene="bla"
/note="AmpR promoter"
CDS 1531..2391
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2562..3150
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggctcattca
301 aagcacggtc taaaagaaga aatgacaatg aaataccaca tggaagggtg cgtcaacgga
361 cataaatttg tgatcacggg cgaaggcatt ggatatccgt tcaaagggaa acagactatt
421 aatctgtgtg tgatcgaagg gggaccattg ccattttccg aagacatatt gtcagctggc
481 tttaagtacg gagacaggat tttcactgaa tatcctcaag acatagtaga ctatttcaag
541 aactcgtgtc ctgctggata tacatggggc aggtcttttc tctttgagga tggagcagtc
601 tgcatatgca atgtagatat aacagtgagt gtcaaagaaa actgcattta tcataagagc
661 atatttaatg gagtgaattt tcctgctgat ggacctgtga tgaaaaagat gacaactaac
721 tgggaagcat cctgcgagaa gatcatgcca gtacctaagc aggggatact gaaaggggat
781 gtctccatgt acctccttct gaaggatggt gggcgttacc ggtgccagtt cgacacagtt
841 tacaaagcaa agtctgtgcc aagtaagatg ccggagtggc acttcatcca gcataagctc
901 ctccgtgaag accgcagcga tgctaagaat cagaagtggc agctgacaga gcatgctatt
961 gcattccctt ctgccttggc ctgaagcggc cgcgactcta gaattccaac tgagcgccgg
1021 tcgctaccat taccaacttg tctggtgtca aaaataatag gcctactagt cggccgtacg
1081 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
1141 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
1201 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
1261 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
1321 gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
1381 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
1441 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
1501 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
1561 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1621 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1681 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1741 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
1801 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1861 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1921 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1981 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
2041 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
2101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
2161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
2221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
2281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
2341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
2401 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2701 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
2941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
3001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
3061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
3121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
3241 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3301 cgcagcgagt cagtgagcga ggaagcggaa g
//