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pET-11d(+)

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聯系方式：I47-825O-882O

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pET-11d(+)載體 pet11d質粒基本信息

出品公司: EMD Biosciences(Novagen)
別名: pET11d, pet 11d
質粒類型: 大腸桿菌蛋白表達
表達水平: 高
克隆方法: 多克隆位點，限制性內切酶
載體大小: 5674bp
5' 測序引物: T7
5' 測序引物序列:   5'-TAATACGACTCACTATAGGG-3'
載體標簽: N-T7
載體抗性: Ampicillin
備注:
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
產品目錄號: 69439-3
穩定性: 瞬時表達 Transient
組成型: 組成型 Constitutive
病毒/非病毒: 非病毒
表達宿主菌: BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)
pET表達手冊： pET表達手冊英文版
pET表達手冊中文版
pET載體匯總:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET-11d(+)質粒圖譜 pet11d載體圖譜和pET-11d(+)載體序列 pet11d質粒序列多克隆位點信息

pet11d載體圖譜
pet11d多克隆位點

Feature Name Start End  
T7_terminator 316 188
T7_Terminal_primer 256 274
T7_leader 358 326
T7_transl_en_RBS 382 366
lacO 427 400
T7_promoter 445 427
pBRrevBam_primer 548 529
lacI 823 1914
tet (576 - 1190) 1973 2587
ROP 3226 3417
pGEX_3_primer 3433 3411
Ampicillin 5466 4606
AmpR_promoter 5536 5508

ORF Start End  
ORF frame 3 582 1082
ORF frame 1 955 1914
ORF frame 1 2392 1697
ORF frame 2 1970 2587
ORF frame 2 5466 4606

Enzyme Name Cut
ClaI 24
HindIII 29
BamHI 319
NheI 350
NcoI 355
XbaI 394
BglII 460
BclI 1196
ApaI 1393
HpaI 1688
EagI 2250
NruI 2285
MscI 2757
PstI 4924
AatII 5601
EcoRI 5672

pET-11d(+)質粒 pet11d載體簡介

pET-11a-d載體具有N端T7標簽和BamH I多克隆克隆位點。這些載體是很多pET家族載體的前身。pET-21a-d(+)系列載體分別對應于pET-11a-d載體，但是包含有幾個額外的特征序列。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。

  pET Vectors 選擇指南
 

pET-11d(+)質粒序列 pet11d載體序列

LOCUS       pET11d    5674 bp     DNA  circular   SYN
DEFINITION  pET11d
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|gt12365.cn|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="pET11d"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(326..358)
                     /label="T7_leader"
     misc_feature    complement(366..382)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(400..427)
                     /label="lacO"
     promoter        complement(427..445)
                     /label="T7_promoter"
     misc_feature    complement(529..548)
                     /label="pBRrevBam_primer"
     CDS             582..1082
                     /label="ORF frame 3"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    823..1914
                     /label="lacI"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             955..1914
                     /label="ORF frame 1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1697..2392)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             1970..2587
                     /label="ORF frame 2"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            1973..2587
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    3226..3417
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(3411..3433)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(4606..5466)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(4606..5466)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5508..5536)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
  181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
  241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
  301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatgg
  361 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
  421 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
  481 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
  541 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
  601 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
  661 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
  721 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
  781 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
  841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
  901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
  961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
 1021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
 1081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
 1141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
 1201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
 1261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
 1321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
 1381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
 1441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
 1501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
 1561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
 1621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
 1681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
 1741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
 1801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
 1861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
 1921 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
 1981 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
 2041 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
 2101 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
 2161 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
 2221 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
 2281 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
 2341 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
 2401 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt
 2461 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
 2521 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
 2581 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
 2641 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc
 2701 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca
 2761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
 2821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
 2881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
 2941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
 3001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
 3061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
 3121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
 3181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
 3241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
 3301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
 3361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
 3421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
 3481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
 3541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
 3601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
 3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
 3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
 3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
 3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
 3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
 3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
 4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
 4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
 4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
 4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
 4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
 4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
 4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
 4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
 4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
 4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
 4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
 4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
 4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
 4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
 4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
 4921 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
 4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
 5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
 5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
 5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
 5221 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
 5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
 5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
 5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
 5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
 5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
 5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
 5641 taggcgtatc acgaggccct ttcgtcttca agaa
//

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