成人做爰A片免费看网站网豆传媒,成人做爰黄级A片免费看土方,单身妈妈韩剧免费大全,女性hpv最初的征兆,被同桌摸到流水的黄文

pET-19b

價格：800元

聯系方式：I47-825O-882O

相關技術服務：質粒構建    基因合成    質粒大提

買家導航

pET-19b載體 pET19b質粒基本信息

出品公司: EMD Biosciences(Novagen)
別名: pET19b, pet 19b
質粒類型: 大腸桿菌蛋白表達
表達水平: 高
克隆方法: 多克隆位點，限制性內切酶
載體大小: 5717bp
5' 測序引物: T7
5' 測序引物序列:   5'-TAATACGACTCACTATAGGG-3'
載體標簽: N-His, N-EK 蛋白酶切位點
載體抗性: Ampicillin
備注:
Hosts: E.coli. Related vectors: pBR322.
產品目錄號: 69677-3
穩定性: 瞬時表達 Transient
組成型: 組成型 Constitutive
病毒/非病毒: 非病毒
表達宿主菌: BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)
pET表達手冊： pET表達手冊英文版
pET表達手冊中文版
pET載體匯總:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET-19b質粒圖譜 pET19b載體圖譜和pET-19b載體序列 pET19b質粒序列多克隆位點信息

pET-19b載體圖譜



pET-19b多克隆位點

pET-19b載體特征

Feature Name Start End  
T7_terminator 316 188
T7_Terminal_primer 256 274
EK 347 336
T7_transl_en_RBS 425 409
lacO 470 443
T7_promoter 488 470
pBRrevBam_primer 591 572
lacI 866 1957
tet (576 - 1190) 2016 2630
ROP 3269 3460
pGEX_3_primer 3476 3454
Ampicillin 5509 4649
AmpR_promoter 5579 5551
ORF Start End  
ORF frame 1 625 1125
ORF frame 2 998 1957
ORF frame 1 2435 1740
ORF frame 3 2013 2630
ORF frame 2 5509 4649
Enzyme Name Cut
ClaI 24
HindIII 29
BamHI 319
XhoI 324
NdeI 331
NcoI 398
XbaI 437
BglII 503
BclI 1239
ApaI 1436
HpaI 1731
EagI 2293
NruI 2328
MscI 2800
PstI 4967
AatII 5644
EcoRI 5715

pET-19b質粒 pET19b載體簡介

pET-19b載體具有N端His標簽和EK蛋白酶切位點。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。
pET Vectors 選擇指南

pET-19b質粒序列 pET19b載體序列

LOCUS       pET-19b    5717 bp     DNA circular    SYN
DEFINITION  pET-19b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     ORIGDB|GenBank|
COMMENT     VNTAUTHORNAME|gt12365.cn|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="pET-19b"
                     /mol_type="other DNA"
     terminator      complement(188..316)
                     /label="T7_terminator"
     misc_feature    256..274
                     /label="T7_Terminal_primer"
     misc_feature    complement(336..347)
                     /label="EK"
     misc_feature    complement(409..425)
                     /label="T7_transl_en_RBS"
     misc_feature    complement(443..470)
                     /label="lacO"
     promoter        complement(470..488)
                     /label="T7_promoter"
     misc_feature    complement(572..591)
                     /label="pBRrevBam_primer"
     CDS             625..1125
                     /label="ORF frame 1"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     misc_feature    866..1957
                     /label="lacI"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
     CDS             998..1957
                     /label="ORF frame 2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(1740..2435)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
                     TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
                     GPRWFAPAGENPV*"
     CDS             2013..2630
                     /label="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            2016..2630
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    3269..3460
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(3454..3476)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(4649..5509)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(4649..5509)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5551..5579)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
  181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
  241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
  301 cgggctttgt tagcagccgg atcctcgagc atatgcttgt cgtcgtcgtc gatatggccg
  361 ctgctgtgat gatgatgatg atgatgatga tgatggccca tggtatatct ccttcttaaa
  421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
  481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
  541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
  601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
  661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
  721 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
  781 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
  841 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
  901 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
  961 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
 1021 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
 1081 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
 1141 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
 1201 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
 1261 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
 1321 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
 1381 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
 1441 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
 1501 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
 1561 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
 1621 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
 1681 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
 1741 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
 1801 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
 1861 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
 1921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
 1981 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
 2041 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
 2101 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
 2161 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
 2221 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
 2281 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
 2341 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
 2401 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
 2461 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
 2521 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
 2581 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
 2641 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
 2701 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
 2761 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
 2821 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
 2881 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
 2941 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
 3001 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
 3061 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
 3121 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
 3181 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
 3241 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
 3301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
 3361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
 3421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
 3481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
 3541 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
 3601 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
 3661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
 3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
 3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
 3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
 3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
 3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
 4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
 4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
 4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
 4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
 4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
 4321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
 4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
 4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
 4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
 4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
 4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
 4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
 4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
 4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
 4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
 4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
 4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
 5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
 5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
 5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
 5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
 5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
 5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
 5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
 5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
 5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
 5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
 5641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
 5701 cctttcgtct tcaagaa
//

pET-19b載體圖譜 pET19b質粒圖譜pdf版和pET-19b載體序列 pET19b質粒序列等相關資料下載

pET-19b質粒 pET19b載體應用舉例

分享到：

全部質粒載體分類

相關質粒載體

相關質粒載體文章

相關載體質粒問答

Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng